Influence of zhuqin formula extract on the cellular and humoral immune response in cyclophamide-immunocompromised mice
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- 英文论文题名：Influence of zhuqin formula extract on the cellular and humoral immune response in cyclophamide-immunocompromised mice
- 论文作者：Z.M.Xun ; L.Juan ; Q.Hong ; Z.Z.Rong ; L.Y.Chen
- 英文论文作者：Z.M.Xun ; L.Juan ; Q.Hong ; Z.Z.Rong ; L.Y.Chen ; Chongqing Academy of Animal Sciences ; Department of Veterinary Medicine ; Southwest University
- 年：2016
- 作者机构：Chongqing Academy of Animal Sciences;Department of Veterinary Medicine,Southwest University;
- 英文论文关键词：cyclophosphamide ; immunosuppression ; spleen ; thymus ; cytokine ; mice.
- 会议召开时间：2016-05-25
- 会议录名称：第七届中国畜牧科技论坛论文集
- 英文会议录名称：Proceeding of the 7th China Animal Husbandry Sci-tech Forum
- 语种：英文
- 分类号：S853.7
- 学会代码：ZGXJ
- 会议名称：第七届中国畜牧科技论坛
- 会议地点：中国重庆
- 主办单位：中国畜牧兽医学会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：51k
- 原文格式：O
- 会议级别：全国
摘要
The aim of this study was to evaluate efficacy of extract from traditional Chinese medicine Zhuqin formula(ZQE) in cyclophosphamide-immunosuppressed mice.A total of 340 four weeks old Kunming mice were taken for the study.The study is divided into two parts.One part is tetermine a safe dose of ZQE by gastric perfusion in mice with an acute toxicity and a maximum tolerance test,and the second part of the presented study indicates the immunorestorative effects of ZQE dependent of the doses applied in mice treated with cyclophosphamide,including the carbon clearance index,the weight ratio of immune organ,serum hemolysin,T-,B-lymphocyte transformation rate and IL-2,IFN-γ,and IL-4 secretion.The acute toxicity test indicated that LD_(50) of ZQE by gastric perfusion in mice was>5 000 mg/kg.The maximum tolerated dose of ZQE(oral administration) was>120 g/kg.In thetreating immunosuppressed mice test,three doses of ZQE groups could increase the carbon clearance index,the weight ratios of spleen and thymus,serum hemolysin level,promote the secretion of IL-2,IL-4,and IFN-γ,and T-,B-lymphocyte transformation rate by 1.81-,1.73-,2.67-,2.36-,1.96-,and 2.69-fold of model group,respectively,and a 30mg/kg dose of ZQE presented a significantly better outcome,compared with the 40 and 20 mg/kg doses.
The aim of this study was to evaluate efficacy of extract from traditional Chinese medicine Zhuqin formula(ZQE) in cyclophosphamide-immunosuppressed mice.A total of 340 four weeks old Kunming mice were taken for the study.The study is divided into two parts.One part is tetermine a safe dose of ZQE by gastric perfusion in mice with an acute toxicity and a maximum tolerance test,and the second part of the presented study indicates the immunorestorative effects of ZQE dependent of the doses applied in mice treated with cyclophosphamide,including the carbon clearance index,the weight ratio of immune organ,serum hemolysin,T-,B-lymphocyte transformation rate and IL-2,IFN-γ,and IL-4 secretion.The acute toxicity test indicated that LD_(50) of ZQE by gastric perfusion in mice was>5 000 mg/kg.The maximum tolerated dose of ZQE(oral administration) was>120 g/kg.In thetreating immunosuppressed mice test,three doses of ZQE groups could increase the carbon clearance index,the weight ratios of spleen and thymus,serum hemolysin level,promote the secretion of IL-2,IL-4,and IFN-γ,and T-,B-lymphocyte transformation rate by 1.81-,1.73-,2.67-,2.36-,1.96-,and 2.69-fold of model group,respectively,and a 30mg/kg dose of ZQE presented a significantly better outcome,compared with the 40 and 20 mg/kg doses.
The aim of this study was to evaluate efficacy of extract from traditional Chinese medicine Zhuqin formula(ZQE) in cyclophosphamide-immunosuppressed mice.A total of 340 four weeks old Kunming mice were taken for the study.The study is divided into two parts.One part is tetermine a safe dose of ZQE by gastric perfusion in mice with an acute toxicity and a maximum tolerance test,and the second part of the presented study indicates the immunorestorative effects of ZQE dependent of the doses applied in mice treated with cyclophosphamide,including the carbon clearance index,the weight ratio of immune organ,serum hemolysin,T-,B-lymphocyte transformation rate and IL-2,IFN-γ,and IL-4 secretion.The acute toxicity test indicated that LD_(50) of ZQE by gastric perfusion in mice was>5 000 mg/kg.The maximum tolerated dose of ZQE(oral administration) was>120 g/kg.In thetreating immunosuppressed mice test,three doses of ZQE groups could increase the carbon clearance index,the weight ratios of spleen and thymus,serum hemolysin level,promote the secretion of IL-2,IL-4,and IFN-γ,and T-,B-lymphocyte transformation rate by 1.81-,1.73-,2.67-,2.36-,1.96-,and 2.69-fold of model group,respectively,and a 30mg/kg dose of ZQE presented a significantly better outcome,compared with the 40 and 20 mg/kg doses.
引文