雨生红球藻多糖脱蛋白工艺研究
详细信息 查看官网全文
- 英文论文题名：Research on Protein Removal in Haematococcus pluvialis Polysaccharide
- 论文作者：张苗 ; 刘晓娟 ; 刘欣
- 英文论文作者：ZHANG Miao ; LIU Xiao-juan ; LIU Xin ; College of Food Science ; South China Agricultural University
- 年：2016
- 作者机构：华南农业大学食品学院;
- 论文关键词：雨生红球藻 ; 多糖 ; 脱蛋白
- 英文论文关键词：Haematococcus pluvialis ; polysaccharide ; deproteinization
- 会议召开时间：2016-12-12
- 会议录名称：“健康食品与功能性食品配料”学术研讨会暨2016年广东省食品学会年会论文集
- 语种：中文
- 分类号：TS201.2
- 学会代码：GYSP
- 会议名称：“健康食品与功能性食品配料”学术研讨会暨2016年广东省食品学会年会
- 会议地点：中国广东广州
- 主办单位：广东省食品学会
- 学会名称：广东省食品学会
- 页数：5
- 文件大小：353k
- 原文格式：O
- 会议级别：全国
摘要
藻类多糖纯化过程中脱蛋白是较为重要的环节之一,为了提高多糖纯化效率,对雨生红球藻多糖脱蛋白的工艺进行了优化研究。在单因素试验基础上,采用正交实验确定了雨生红球藻多糖Sevag法脱除蛋白的最佳工艺条件。结果表明,采用Sevag法脱除蛋白的最佳工艺条件为:每次振摇20min,氟仿与正丁醇体积比5:1,多糖提取液与Sevag液的体积比4:1,脱蛋白次数5次,蛋白脱除率及多糖含量分别为88.14%和34.85%。综上所述,采用Sevag法脱除蛋白是雨生红球藻多糖脱蛋白的有效方法,为藻多糖的进一步开发提供参考。
Removing protein is one of the important elements in the process of algae polysaccharide purification.In order to improve the efficiency of polysaccharide purification,the present paper mainly investigated the technology of protein removal from Haematococcus pluvialis polysaccharide,so as to optimize the process of de-protein.Operating parameters for deprotemization by Sevag method of polysaccharide from Haematococcus pluvialis were optimized using an orthogonal array design.The optimal conditions for deproteinizing crude polysaccharide by Sevag's method were found to be five treatment cycles with a mixture of chloroform and n-butyl alcohol(5:1,V/V)at a solid-to-solvent ratio of 1:4(g/mL).Under these conditions,the deprotemization rate and the polysaccharide content were 88.14% and 34.85%,respectively.The results showed that Sevag method is a potential effective way to remove Haematococcus pluvialis polysaccharide protein,and providing references for the development and utilization of Haematococcus pluvialis polysaccharide.
Removing protein is one of the important elements in the process of algae polysaccharide purification.In order to improve the efficiency of polysaccharide purification,the present paper mainly investigated the technology of protein removal from Haematococcus pluvialis polysaccharide,so as to optimize the process of de-protein.Operating parameters for deprotemization by Sevag method of polysaccharide from Haematococcus pluvialis were optimized using an orthogonal array design.The optimal conditions for deproteinizing crude polysaccharide by Sevag's method were found to be five treatment cycles with a mixture of chloroform and n-butyl alcohol(5:1,V/V)at a solid-to-solvent ratio of 1:4(g/mL).Under these conditions,the deprotemization rate and the polysaccharide content were 88.14% and 34.85%,respectively.The results showed that Sevag method is a potential effective way to remove Haematococcus pluvialis polysaccharide protein,and providing references for the development and utilization of Haematococcus pluvialis polysaccharide.
引文
[1]张睿钦,管斌,孔青,等.雨生红球藻异养转化产虾青素的条件研究[J].浙江大学学报:农业与生命科学版,2011,37(6):624-630.
[2]Kamath B S,Vidhyavathi R,Sarada R,et al.Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants[J].Bioresource technology,2008,99(18):8667-8673.
[3]Pu J,Bankston J D,Sathivel S.Developing microencapsulated flaxseed oil containing shrimp(Litopenaeus setiferus)astaxanthin using a pilot scale spray dryer[J].Biosystems engineering,2011,108(2):121-132.
[4]Katsumata T,Ishibashi T,Kyle D.A sub-chronic toxicity evaluation of a natural astaxanthin-rich carotenoid extract of Paracoccus carotinifaciens in rats[J].Toxicology Reports,2014,1:582-588.
[5]Sowmya R,Sachindra N M.Evaluation of antioxidant activity of carotenoid extract from shrimp processing byproducts by in vitro assays and in membrane model system[J].Food Chemistry,2012,134(1):308-314.
[6]Monroy-Ruiz J,Sevilla M A,Carron R,et al.Astaxanthin-enriched-diet reduces blood pressure and improves cardiovascular parameters in spontaneously hypertensive rats[J].Pharmacological research,2011,63(1):44-50.
[7]Reyes F A,Mendiola J A,Ibanez E,et al.Astaxanthin extraction from Haematococcus pluvialis using CO_2-expanded ethanol[J].The Journal of Supercritical Fluids,2014,92:75-83.
[8]冯以明,李广生,吴建东,等.雨生红球藻多糖的提取分离及理化性质研究[J].海洋科学,2012,1:17-22.
[9]张苗,刘晓娟,刘欣.超声辅助提取雨生红球藻渣多糖工艺优化[J].食品工业科技,2016,(20):254-262.
[10]伍善广,赖泰君,孙建华,等.蚕蛹多糖脱蛋白方法研究[J].食品科学,2011,32(14):21-24.
[11]扈瑞平,张兴夫,杜玲,等.沙葱多糖Sevage法除蛋白工艺的研究[J].内蒙古大学学报:自然科学版,2009,40(6):658-662.
[12]刘玉佳,孔繁东,刘兆芳,等.桔梗多糖Sevag法除蛋白工艺的研究[J].中国调味品,2014,39(4):5-7.
[13]Dubois M,Gilles K A,Hamilton J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical chemistry,1956,28(3):350-356.
[14]李建武,余瑞元,袁明秀,等.生物化学实验原理和方法[M].北京:北京大学出版社,2000,174-177.
[2]Kamath B S,Vidhyavathi R,Sarada R,et al.Enhancement of carotenoids by mutation and stress induced carotenogenic genes in Haematococcus pluvialis mutants[J].Bioresource technology,2008,99(18):8667-8673.
[3]Pu J,Bankston J D,Sathivel S.Developing microencapsulated flaxseed oil containing shrimp(Litopenaeus setiferus)astaxanthin using a pilot scale spray dryer[J].Biosystems engineering,2011,108(2):121-132.
[4]Katsumata T,Ishibashi T,Kyle D.A sub-chronic toxicity evaluation of a natural astaxanthin-rich carotenoid extract of Paracoccus carotinifaciens in rats[J].Toxicology Reports,2014,1:582-588.
[5]Sowmya R,Sachindra N M.Evaluation of antioxidant activity of carotenoid extract from shrimp processing byproducts by in vitro assays and in membrane model system[J].Food Chemistry,2012,134(1):308-314.
[6]Monroy-Ruiz J,Sevilla M A,Carron R,et al.Astaxanthin-enriched-diet reduces blood pressure and improves cardiovascular parameters in spontaneously hypertensive rats[J].Pharmacological research,2011,63(1):44-50.
[7]Reyes F A,Mendiola J A,Ibanez E,et al.Astaxanthin extraction from Haematococcus pluvialis using CO_2-expanded ethanol[J].The Journal of Supercritical Fluids,2014,92:75-83.
[8]冯以明,李广生,吴建东,等.雨生红球藻多糖的提取分离及理化性质研究[J].海洋科学,2012,1:17-22.
[9]张苗,刘晓娟,刘欣.超声辅助提取雨生红球藻渣多糖工艺优化[J].食品工业科技,2016,(20):254-262.
[10]伍善广,赖泰君,孙建华,等.蚕蛹多糖脱蛋白方法研究[J].食品科学,2011,32(14):21-24.
[11]扈瑞平,张兴夫,杜玲,等.沙葱多糖Sevage法除蛋白工艺的研究[J].内蒙古大学学报:自然科学版,2009,40(6):658-662.
[12]刘玉佳,孔繁东,刘兆芳,等.桔梗多糖Sevag法除蛋白工艺的研究[J].中国调味品,2014,39(4):5-7.
[13]Dubois M,Gilles K A,Hamilton J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical chemistry,1956,28(3):350-356.
[14]李建武,余瑞元,袁明秀,等.生物化学实验原理和方法[M].北京:北京大学出版社,2000,174-177.