液相色谱质谱法测定洗发水中去屑因子吡硫翁锌和甘宝素在头皮上的沉积量
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- 英文论文题名：Ultra-high-performance-liquid-chromatography-tandem mass spectrometry measurement of zinc pyrithione and climbazole deposition from hair care products onto human scalp
- 论文作者：陈国强 ; 朱介兵
- 英文论文作者：Guoqiang Chen ; Jiebing Zhu ; Unilever R&D Shanghai
- 年：2016
- 作者机构：联合利华上海研发中心;
- 论文关键词：吡硫翁锌 ; 甘宝素 ; 去屑洗发水 ; 沉积 ; 头皮 ; 液相色谱质谱法
- 英文论文关键词：Zinc pyrithione ; climbazole ; anti-dandruff shampoo ; deposition ; scalp ; UHPLC-MS/MS
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：O657.63;TQ658.33
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：7
- 文件大小：759k
- 原文格式：O
- 会议级别：全国
摘要
使用去屑洗发水后,检测头皮上沉积的去屑因子的量,可以帮助判断去屑效果。本文建立了测定洗发水中去屑成分吡硫翁锌(ZnPT)和甘宝素(CBZ)在头皮上的沉积量的液相色谱质谱方法。缓冲液清洗(buffer scrub)作为临床取样方法,在受试者使用测试用去屑洗发水后,采集头皮样品。ZnPT经过衍生化和CBZ同时被液相色谱质谱法定量。该方法可以测定头皮上纳克级的ZnPT和CBZ。采用这个方法,成功测定了在使用单去屑因子(ZnPT)和双去屑因子(ZnPT和CBZ)洗发水后不同时间点,头皮上ZnPT和CBZ的沉积量。
The measurement of anti-dandruff(AD) actives deposited onto the scalp from AD shampoos is helpful to evaluate the AD efficacy.A sensitive ultrahigh performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method has been developed and validated for simultaneous quantification of zinc pyrithione(ZnPT) and climbazole(CBZ) deposited onto human scalp from AD shampoos.Scrubbing with a buffer solution was used as the sampling method for the extraction of ZnPT and CBZ from scalp.Derivatization of ZnPT was carried out prior to UHPLC-MS/MS analysis.The limit of detection for ZnPT and CBZ was established to be 1 and 2 ng/mL,respectively.This sensitivity enables the quantification of ZnPT and CBZ at deposition levels in the low ng/cm2 range.The method was successfully applied for the analysis of scalp buffer scrub samples from an in vivo study.The levels of ZnPT and CBZ deposited on the scalp at different time points after application of the AD shampoo were measured.
The measurement of anti-dandruff(AD) actives deposited onto the scalp from AD shampoos is helpful to evaluate the AD efficacy.A sensitive ultrahigh performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS) method has been developed and validated for simultaneous quantification of zinc pyrithione(ZnPT) and climbazole(CBZ) deposited onto human scalp from AD shampoos.Scrubbing with a buffer solution was used as the sampling method for the extraction of ZnPT and CBZ from scalp.Derivatization of ZnPT was carried out prior to UHPLC-MS/MS analysis.The limit of detection for ZnPT and CBZ was established to be 1 and 2 ng/mL,respectively.This sensitivity enables the quantification of ZnPT and CBZ at deposition levels in the low ng/cm2 range.The method was successfully applied for the analysis of scalp buffer scrub samples from an in vivo study.The levels of ZnPT and CBZ deposited on the scalp at different time points after application of the AD shampoo were measured.
引文
1.Turner G A,Matheson J R,Li G Z,etc.Enhanced Sensory Properties of an Anti-dandruff Shampoo Containing Zinc Pyrithione and Climbazole,Int.J.Cosmet.Sci.35(2013)78.
2.Schwartz J R,Shah R,Krigbaum H,etc.New Insights on Dandruff/Seborrhoeic Dermatitis:the Role of the Scalp Follicular Infundibulum in Effective Treatment Strategies,Br.J.Dermatol.165(2011)18.
3.Schwartz J R,Bacon R A,Shah R,etc.Therapeutic Efficacy of Anti-dandruff Shampoos:a Randomized Clinical Trial Comparing Products Based on Potentiated Zinc Pyrithione and Zinc pyrithione/Climbazole,Int.J.Cosmet.Sci.35(2013)381.
4.Chao L,Simultaneous Determination of Four Anti-drandruff Agents Including Octopirox in Shampoo Products by Reversed Liquid Chromatography,Int.J.Cosmet.Sci.23(2001)183.
5.Gagliardi L,Turchetto L,Amato A,Determination of Climbazol in Shampoos by Reversed-phase Liquid Chromatography,Anal.Chim.Acta.235(1990)465.
6.Fenn R J,Alexander M T,Determination of Zinc Pyrithione in Hair Care Products by Normal Phase Liquid Chromatography,J.Liq.Chrom.11(1988)3403.
7.Shih Y,Zen J,Kumar A S,etc.Flow Injection Analysis of Zinc Pyrithione in Hair Care Products on a Cobalt Phthalocyanine Modified Screen-printed Carbon Electrode,Talanta 62(2004)912.
8.Cheng H,Gadde R R,Analysis of Zinc Pyrithione in Shampoos by Reversed-phase High-performance Liquid Chromatography,J Chromatogr.291(1984)434.
9.Pena-Méndez E M,Havel J,Malecek J,High-performance Capillary Electrophoresis Determination of Pyrithione in Antidandruff Preparations and Shampoos,J.Capillary Electrophor.4(1997)269.
10.Nakajima K,Yasuda T,Nakazawa H,High-performance Liquid Chromatographic Determination of Zinc Pyrithione in Antidandruff Preparations Based on Copper Chelate Formation,J.Chromatogr.A 502(1990)379.
11.Wick A,Fink G,Ternes T A,Comparison of Electrospray Ionization and Atmospheric Pressure Chemical Ionization for Multi-residue Analysis of Biocides,UV-filters and Benzothiazoles in Aqueous Matrices and Activated Sludge by Liquid Chromatography-tandem Mass Spectrometry,J.Chromatogr.A 1217(2010)2008.
12.Chen Z F,Ying G G,Lai H J,etc.Determination of Biocides in Different Environmental Matrices by Use of Ultra-high-performance Liquid Chromatography-tandem Mass Spectrometry,Anal.Bioanal.Chem.,404(2012)3175.
13.Bones J,Thomas K V,Paull B,Improved Method for the Determination of Zinc Pyrithione in Environmental Water Samples Incorporating On-line Extraction and Preconcentration Coupled with Liquid Chromatography Atmospheric Pressure Chemical Ionisation Mass Spectrometry,J.Chromatogr.A1132(2006)157.
14.Sakkas V A,Shibata K,Yamaguchi Y,etc.Aqueous Phototransformation of Zinc Pyrithione Degradation Kinetics and Byproduct Identification by Liquid Chromatography-atmospheric Pressure Chemical Ionisation Mass Spectrometry,J.Chromatogr.A1144(2007)176.
15.Chen G,Hoptroff M,Fei X,etc.Ultra-high-performance Liquid Chromatography-tandem Mass Spectrometry Measurement of Climbazole Deposition from Hair Care Products onto Artificial Skin and Human Scalp,J.Chromatogr.A1317(2013)155.
16.Harding C R,Moore A E,Rogers J S,etc.Dandruff:a Condition Characterized by Decreased Levels of Intercellular Lipids in Stratum Corneum and Impaired Barrier Function,Arch.Dermatol.Res.,294(2002)221.
17.Yamaguchi Y,Kumakura A,Sugasawa S,etc.Direct Analysis of Zinc Pyrithione Using LC-MS,Intern.J.Environ.Anal.Chem.86(2006)83.
18.Voulvoulis N,Scrimshaw M D,Lester J N,etc.Analytical Methods for the Detection of 9Antifouling Paint Booster Biocides in Estuarine Water Samples,Chemosphere 38(1999)3503.
19.Hay R J,Malassezia,Dandruff and Seborrhoeic Dermatitis:an Overview,Br.J.Dermatol.165(2011)2.
2.Schwartz J R,Shah R,Krigbaum H,etc.New Insights on Dandruff/Seborrhoeic Dermatitis:the Role of the Scalp Follicular Infundibulum in Effective Treatment Strategies,Br.J.Dermatol.165(2011)18.
3.Schwartz J R,Bacon R A,Shah R,etc.Therapeutic Efficacy of Anti-dandruff Shampoos:a Randomized Clinical Trial Comparing Products Based on Potentiated Zinc Pyrithione and Zinc pyrithione/Climbazole,Int.J.Cosmet.Sci.35(2013)381.
4.Chao L,Simultaneous Determination of Four Anti-drandruff Agents Including Octopirox in Shampoo Products by Reversed Liquid Chromatography,Int.J.Cosmet.Sci.23(2001)183.
5.Gagliardi L,Turchetto L,Amato A,Determination of Climbazol in Shampoos by Reversed-phase Liquid Chromatography,Anal.Chim.Acta.235(1990)465.
6.Fenn R J,Alexander M T,Determination of Zinc Pyrithione in Hair Care Products by Normal Phase Liquid Chromatography,J.Liq.Chrom.11(1988)3403.
7.Shih Y,Zen J,Kumar A S,etc.Flow Injection Analysis of Zinc Pyrithione in Hair Care Products on a Cobalt Phthalocyanine Modified Screen-printed Carbon Electrode,Talanta 62(2004)912.
8.Cheng H,Gadde R R,Analysis of Zinc Pyrithione in Shampoos by Reversed-phase High-performance Liquid Chromatography,J Chromatogr.291(1984)434.
9.Pena-Méndez E M,Havel J,Malecek J,High-performance Capillary Electrophoresis Determination of Pyrithione in Antidandruff Preparations and Shampoos,J.Capillary Electrophor.4(1997)269.
10.Nakajima K,Yasuda T,Nakazawa H,High-performance Liquid Chromatographic Determination of Zinc Pyrithione in Antidandruff Preparations Based on Copper Chelate Formation,J.Chromatogr.A 502(1990)379.
11.Wick A,Fink G,Ternes T A,Comparison of Electrospray Ionization and Atmospheric Pressure Chemical Ionization for Multi-residue Analysis of Biocides,UV-filters and Benzothiazoles in Aqueous Matrices and Activated Sludge by Liquid Chromatography-tandem Mass Spectrometry,J.Chromatogr.A 1217(2010)2008.
12.Chen Z F,Ying G G,Lai H J,etc.Determination of Biocides in Different Environmental Matrices by Use of Ultra-high-performance Liquid Chromatography-tandem Mass Spectrometry,Anal.Bioanal.Chem.,404(2012)3175.
13.Bones J,Thomas K V,Paull B,Improved Method for the Determination of Zinc Pyrithione in Environmental Water Samples Incorporating On-line Extraction and Preconcentration Coupled with Liquid Chromatography Atmospheric Pressure Chemical Ionisation Mass Spectrometry,J.Chromatogr.A1132(2006)157.
14.Sakkas V A,Shibata K,Yamaguchi Y,etc.Aqueous Phototransformation of Zinc Pyrithione Degradation Kinetics and Byproduct Identification by Liquid Chromatography-atmospheric Pressure Chemical Ionisation Mass Spectrometry,J.Chromatogr.A1144(2007)176.
15.Chen G,Hoptroff M,Fei X,etc.Ultra-high-performance Liquid Chromatography-tandem Mass Spectrometry Measurement of Climbazole Deposition from Hair Care Products onto Artificial Skin and Human Scalp,J.Chromatogr.A1317(2013)155.
16.Harding C R,Moore A E,Rogers J S,etc.Dandruff:a Condition Characterized by Decreased Levels of Intercellular Lipids in Stratum Corneum and Impaired Barrier Function,Arch.Dermatol.Res.,294(2002)221.
17.Yamaguchi Y,Kumakura A,Sugasawa S,etc.Direct Analysis of Zinc Pyrithione Using LC-MS,Intern.J.Environ.Anal.Chem.86(2006)83.
18.Voulvoulis N,Scrimshaw M D,Lester J N,etc.Analytical Methods for the Detection of 9Antifouling Paint Booster Biocides in Estuarine Water Samples,Chemosphere 38(1999)3503.
19.Hay R J,Malassezia,Dandruff and Seborrhoeic Dermatitis:an Overview,Br.J.Dermatol.165(2011)2.