Non-SMC elements 1 and 3 are required for early embryo and seedling development in Arabidopsis
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- 英文论文题名：Non-SMC elements 1 and 3 are required for early embryo and seedling development in Arabidopsis
- 论文作者：Gang Li ; Wenxuan Zou ; Liufang Jian ; Jie Qian ; Yingtian Deng ; Jie Zhao
- 英文论文作者：Gang Li ; Wenxuan Zou ; Liufang Jian ; Jie Qian ; Yingtian Deng ; Jie Zhao ; State Key Laboratory of Hybrid Rice ; College of Life Sciences ; Wuhan University
- 年：2016
- 作者机构：State Key Laboratory of Hybrid Rice,College of Life Sciences,Wuhan University;
- 英文论文关键词：non-SMC elements ; embryo ; seedling ; Arabidopsis
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q944.4
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：842k
- 原文格式：D
- 会议级别：全国
摘要
The structural maintenance of the chromosome 5/6(SMC5/6) complex has a variety of functions in mitosis and meiosis in yeast and human,including facilitating homologous recombination,restarting stalled replication forks,and maintaining ribosomal DNA and heterochromatin,among other roles. In Arabidopsis,At SMC5 and At SMC6A/6B are responsive to DNA damage. However,the non-SMC element 1 and 3 functions in Arabidopsis growth and development remain as yet unknown. In our study,it was found that the loss function of the non-SMC element 1 and 3 led to the conversion of suspensor cell fate and caused defects in embryo pattern formation. Partially-complemented homologous mutants showed that the post-embryonic development of the mutants was inhibited,that chromosome fragments occurred during segregation of chromosomes,and that the transition from the G2 phase to the M phase was delayed,leading to endoreduplication. All of these results demonstrate that the non-SMC element 1 and 3 are essential for cell division and function in maintaining the stability of chromosome ploidy in mitosis. Further,a large number of dead cells and DNA DSBs were observed in the their mutants,and the expression of the non-SMC element 1 and 3 were up-regulated following treatment of the plants with DSBs inducer compounds,indicating that the non-SMC element 1 and 3 play roles in DNA damage repair. Therefore,we conclude that the nonSMC element 1 and 3 facilitate DSBs repair and contribute to maintaining the stability of chromosome ploidy in mitotic cells. The non-SMC element 1 and 3 could thus be considered as required factors for maintaining proper early embryo cell differentiation and post-embryonic development.
The structural maintenance of the chromosome 5/6(SMC5/6) complex has a variety of functions in mitosis and meiosis in yeast and human,including facilitating homologous recombination,restarting stalled replication forks,and maintaining ribosomal DNA and heterochromatin,among other roles. In Arabidopsis,At SMC5 and At SMC6A/6B are responsive to DNA damage. However,the non-SMC element 1 and 3 functions in Arabidopsis growth and development remain as yet unknown. In our study,it was found that the loss function of the non-SMC element 1 and 3 led to the conversion of suspensor cell fate and caused defects in embryo pattern formation. Partially-complemented homologous mutants showed that the post-embryonic development of the mutants was inhibited,that chromosome fragments occurred during segregation of chromosomes,and that the transition from the G2 phase to the M phase was delayed,leading to endoreduplication. All of these results demonstrate that the non-SMC element 1 and 3 are essential for cell division and function in maintaining the stability of chromosome ploidy in mitosis. Further,a large number of dead cells and DNA DSBs were observed in the their mutants,and the expression of the non-SMC element 1 and 3 were up-regulated following treatment of the plants with DSBs inducer compounds,indicating that the non-SMC element 1 and 3 play roles in DNA damage repair. Therefore,we conclude that the nonSMC element 1 and 3 facilitate DSBs repair and contribute to maintaining the stability of chromosome ploidy in mitotic cells. The non-SMC element 1 and 3 could thus be considered as required factors for maintaining proper early embryo cell differentiation and post-embryonic development.
The structural maintenance of the chromosome 5/6(SMC5/6) complex has a variety of functions in mitosis and meiosis in yeast and human,including facilitating homologous recombination,restarting stalled replication forks,and maintaining ribosomal DNA and heterochromatin,among other roles. In Arabidopsis,At SMC5 and At SMC6A/6B are responsive to DNA damage. However,the non-SMC element 1 and 3 functions in Arabidopsis growth and development remain as yet unknown. In our study,it was found that the loss function of the non-SMC element 1 and 3 led to the conversion of suspensor cell fate and caused defects in embryo pattern formation. Partially-complemented homologous mutants showed that the post-embryonic development of the mutants was inhibited,that chromosome fragments occurred during segregation of chromosomes,and that the transition from the G2 phase to the M phase was delayed,leading to endoreduplication. All of these results demonstrate that the non-SMC element 1 and 3 are essential for cell division and function in maintaining the stability of chromosome ploidy in mitosis. Further,a large number of dead cells and DNA DSBs were observed in the their mutants,and the expression of the non-SMC element 1 and 3 were up-regulated following treatment of the plants with DSBs inducer compounds,indicating that the non-SMC element 1 and 3 play roles in DNA damage repair. Therefore,we conclude that the nonSMC element 1 and 3 facilitate DSBs repair and contribute to maintaining the stability of chromosome ploidy in mitotic cells. The non-SMC element 1 and 3 could thus be considered as required factors for maintaining proper early embryo cell differentiation and post-embryonic development.
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