The effects of IGF1 on the melanogenesis in alpaca melanocytes in vitro
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- 英文论文题名：The effects of IGF1 on the melanogenesis in alpaca melanocytes in vitro
- 论文作者：Shuaipeng Hu ; Yu Liu ; Shanshan Yang ; Kaiyuan Ji ; Xuexian Liu ; Junzhen Zhang ; Ruiwen Fan ; Changsheng Dong
- 英文论文作者：Shuaipeng Hu ; Yu Liu ; Shanshan Yang ; Kaiyuan Ji ; Xuexian Liu ; Junzhen Zhang ; Ruiwen Fan ; Changsheng Dong ; College of Animal Science and Veterinary Medicine ; Shanxi Agricultural University
- 年：2016
- 作者机构：College of Animal Science and Veterinary Medicine,Shanxi Agricultural University;
- 英文论文关键词：IGF1 ; melanogenesis ; melanocyte ; alpaca
- 会议召开时间：2016-08-20
- 会议录名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会论文集
- 语种：英文
- 分类号：S829.9
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会
- 会议地点：中国河南郑州
- 主办单位：中国畜牧兽医学会动物解剖及组织胚胎学分会
- 学会名称：中国畜牧兽医学会
- 页数：2
- 文件大小：113k
- 原文格式：O
- 会议级别：全国
摘要
In order to investigate the effects of the insulin-like growth factor 1(IGF-1) on alpaca melanocyte in vitro,different dosees of IGF1(0ng/ml,10ng/ml,20ng/ml,40ng/ml) were added in the medium of alpaca melanocyte.The RTCA machine was used to monitor the proliferation,quantitative real-time PCR and western blot to test the relative gene expression,ELISA to test cAMP production,and spectrum method to test the melanin production.The results showed that compared to the normal melanocyte,the proliferation of melanocytes was increased within 60 h following adding IGFl.It also showed that cAMP content produced by melanocytes was increased,Microphthalmia-associtated transcription factor(MITF),Tyrosinase(TYR) and Tyrosinase Related protein 2(TYRP2) expression was increased,and melanin production with most obvious change in 10ng/ml supplementary group,when compared with the control group.The results suggested that IGFl with the dose of 10 ng/ml had the important effects on the melanogenesis in alpaca melanocyte by the cAMP pathway.
In order to investigate the effects of the insulin-like growth factor 1(IGF-1) on alpaca melanocyte in vitro,different dosees of IGF1(0ng/ml,10ng/ml,20ng/ml,40ng/ml) were added in the medium of alpaca melanocyte.The RTCA machine was used to monitor the proliferation,quantitative real-time PCR and western blot to test the relative gene expression,ELISA to test cAMP production,and spectrum method to test the melanin production.The results showed that compared to the normal melanocyte,the proliferation of melanocytes was increased within 60 h following adding IGFl.It also showed that cAMP content produced by melanocytes was increased,Microphthalmia-associtated transcription factor(MITF),Tyrosinase(TYR) and Tyrosinase Related protein 2(TYRP2) expression was increased,and melanin production with most obvious change in 10ng/ml supplementary group,when compared with the control group.The results suggested that IGFl with the dose of 10 ng/ml had the important effects on the melanogenesis in alpaca melanocyte by the cAMP pathway.
In order to investigate the effects of the insulin-like growth factor 1(IGF-1) on alpaca melanocyte in vitro,different dosees of IGF1(0ng/ml,10ng/ml,20ng/ml,40ng/ml) were added in the medium of alpaca melanocyte.The RTCA machine was used to monitor the proliferation,quantitative real-time PCR and western blot to test the relative gene expression,ELISA to test cAMP production,and spectrum method to test the melanin production.The results showed that compared to the normal melanocyte,the proliferation of melanocytes was increased within 60 h following adding IGFl.It also showed that cAMP content produced by melanocytes was increased,Microphthalmia-associtated transcription factor(MITF),Tyrosinase(TYR) and Tyrosinase Related protein 2(TYRP2) expression was increased,and melanin production with most obvious change in 10ng/ml supplementary group,when compared with the control group.The results suggested that IGFl with the dose of 10 ng/ml had the important effects on the melanogenesis in alpaca melanocyte by the cAMP pathway.
引文