MET Is Required for the Maximal Action of 20-Hydroxyecdysone during Bombyx Metamorphosis
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- 英文论文题名：MET Is Required for the Maximal Action of 20-Hydroxyecdysone during Bombyx Metamorphosis
- 论文作者：郭恩 ; Qianyu He ; Shumin Liu ; Ling Tian ; Zhentao Sheng ; Qin Peng ; Jingmin Guan ; Mingan Shi ; Kang Li ; Lawrence I.Gilbert ; Jian Wang ; 曹阳 ; 李胜
- 英文论文作者：Enen Guo ; Qianyu He ; Shumin Liu ; Ling Tian ; Zhentao Sheng ; Qin Peng ; Jingmin Guan ; Mingan Shi ; Kang Li ; Lawrence I.Gilbert ; Jian Wang ; Yang Cao ; Sheng Li ; Key Laboratory of Insect Developmental and Evolutionary Biology ; Institute of Plant Physiology and Ecology ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences ; Laboratory of Insect Molecular Biology and Biotechnology ; Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding ; College of Animal Sciences ; South China Agricultural University ; Department of Biology ; University of North Carolina ; Department of Entomology ; University of Maryland ; College Park
- 年：2014
- 作者机构：Key Laboratory of Insect Developmental and Evolutionary Biology,Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences;Laboratory of Insect Molecular Biology and Biotechnology,Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding,College of Animal Sciences,South China Agricultural University;Department of Biology,University of North Carolina;Department of Entomology,University of Maryland,College Park;
- 英文论文关键词：MET ; 20-Hydroxyecdysone ; Bombyx mori ; Metamorphosis
- 会议召开时间：2014-12-19
- 会议录名称：广东省遗传学会第九届代表大会暨学术研讨会论文及摘要汇编
- 英文会议录名称：Theses and Abstracts Compilation of 9th Conference on Genetics,Guangdong
- 语种：英文
- 分类号：Q963
- 学会代码：GDKL
- 会议名称：广东省遗传学会第九届代表大会暨学术研讨会
- 会议地点：中国广东广州
- 主办单位：广东省遗传学会
- 学会名称：广东省科学技术协会科技交流部
- 页数：2
- 文件大小：72k
- 原文格式：O
- 会议级别：地方
摘要
Little is known about how the putative juvenile hormone(JH) receptor,the bHLH-PAS transcription factor MET,is involved in 20-hydroxyecdysone(20E;the molting hormone) action.Here we report that two MET proteins found in the silkworm,Bombyx mori,participate in 20 E signal transduction.Met is 20 E responsive and its expression peaks during molting and pupation,when the 20 E titer is high.As found with results from RNAi knockdown of EcR-USP(the ecdysone receptor genes),RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered transcriptional cascade,preventing tissue remodeling(including autophagy,apoptosis and destruction of larval tissues and generation of adult structures) and causing lethality during the larval-pupal transition.MET physically interacts with EcR-USP.Moreover,MET,EcR-USP and the 20E-response element(EcRE) form a protein-DNA complex,implying that MET might modulate 20E-induced gene transcription by interacting with EcR-USP.In conclusion,the 20 E induction of MET is required for the maximal action of 20 E during Bombyx metamorphosis.
Little is known about how the putative juvenile hormone(JH) receptor,the bHLH-PAS transcription factor MET,is involved in 20-hydroxyecdysone(20E;the molting hormone) action.Here we report that two MET proteins found in the silkworm,Bombyx mori,participate in 20 E signal transduction.Met is 20 E responsive and its expression peaks during molting and pupation,when the 20 E titer is high.As found with results from RNAi knockdown of EcR-USP(the ecdysone receptor genes),RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered transcriptional cascade,preventing tissue remodeling(including autophagy,apoptosis and destruction of larval tissues and generation of adult structures) and causing lethality during the larval-pupal transition.MET physically interacts with EcR-USP.Moreover,MET,EcR-USP and the 20E-response element(EcRE) form a protein-DNA complex,implying that MET might modulate 20E-induced gene transcription by interacting with EcR-USP.In conclusion,the 20 E induction of MET is required for the maximal action of 20 E during Bombyx metamorphosis.
Little is known about how the putative juvenile hormone(JH) receptor,the bHLH-PAS transcription factor MET,is involved in 20-hydroxyecdysone(20E;the molting hormone) action.Here we report that two MET proteins found in the silkworm,Bombyx mori,participate in 20 E signal transduction.Met is 20 E responsive and its expression peaks during molting and pupation,when the 20 E titer is high.As found with results from RNAi knockdown of EcR-USP(the ecdysone receptor genes),RNAi knockdown of Met at the early wandering stage disrupts the 20E-triggered transcriptional cascade,preventing tissue remodeling(including autophagy,apoptosis and destruction of larval tissues and generation of adult structures) and causing lethality during the larval-pupal transition.MET physically interacts with EcR-USP.Moreover,MET,EcR-USP and the 20E-response element(EcRE) form a protein-DNA complex,implying that MET might modulate 20E-induced gene transcription by interacting with EcR-USP.In conclusion,the 20 E induction of MET is required for the maximal action of 20 E during Bombyx metamorphosis.
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