摘要
Background: Lyme disease is caused by Borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of Borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. The prevalence of Borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the Borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of Borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for Borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with Borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of Borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.
Background: Lyme disease is caused by Borrelia burgdorferi. Various animals and wildlife are natural reservoirs of the disease. It is reported that sika deer may as an important reservoir hosts for several species of Borrelia burgdorferi and transmit them to domestic humans and animals. However, little is known about the presence of Borrelia pathogens in sika deer in China. The prevalence of Borrelia spp. in sika deer from Jilin Province in China was assessed. Methods: Seventy-one blood samples of sika deer were collected at four sites from Jilin Province during June and July, 2015, and tested by nested PCR based on the 5s-23 s rRNA, Osp A, Fla and 16 S rRNA genes of the Borrelia burgdorferi. The PCR results were confirmed by DNA sequencing based on long fragment of 16 S rRNA gene. The identification of Borrelia burdorferi species were determined according to obtained gene sequences of selected positive samples from each sampling area. Results: 12 samples were positive for Borrelia spp.(16.9%, 95% CI=10.5-28.8). After sequences alignment, three representative positive sequences from three sites were identified as being infected with Borrelia spp. that have high similarities(96.7-99.6%) with B. garinii species of the strain T-MDJ(HM007279) from China. Conclusion: This is the first report of Borrelia garinii in sike deer from Jilin Province, in China. The data obtained indicates that sika deer might be a nature host and a potential source spreads this pathogen to domestic animals, even human in China.
引文