Effects of CD38 gene knockout on inflammatory factors of mice spleen B-cells
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- 英文论文题名：Effects of CD38 gene knockout on inflammatory factors of mice spleen B-cells
- 论文作者：Li Rong ; Jiao Hui-yuan ; Chen Cheng ; Li Ling ; Dai Qian-qian ; Song Kuang-yu
- 英文论文作者：Li Rong ; Jiao Hui-yuan ; Chen Cheng ; Li Ling ; Dai Qian-qian ; Song Kuang-yu ; Department of Microbiology ; Faculty of Basic Medical Sciences Nanchang University ; Institute of Translational Medicine ; Nanchang University
- 年：2016
- 作者机构：Department of Microbiology,Faculty of Basic Medical Sciences Nanchang University;Institute of Translational Medicine,Nanchang University;
- 英文论文关键词：CD38 gene ; B cell ; inflamation factors ; Sirt1 ; TNF-α ; IL-1β
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：352k
- 原文格式：D
- 会议级别：全国
摘要
Objective: To analysis the number of spleen B-cells and the expression level of inflammatory factors(TNF-α and IL-1β) and Sirt1 in spleen B-cells of CD38-/- mice, to explore the effects of CD38 gene knockout on inflammatory factors of Bcells and its' potential mechanisms. Methods Sorting B-cells from spleens of wide type(WT) C57BL/6 and CD38-/- mice individually by negative sorting method with magnetic beads, counting the cell number, and identifing the purity of sorting B-cells by flow cytometry. cDNA was amplified by RT-PCR, and protein was extracted by RIPA. The mRNA expression levels of inflammatory factors and CD38 gene were detected by Real-time PCR, the protein expression levels of CD38 gene, Neo gene and Sirt1 gene were detected by Western-blot. Results We isolated B-cells from both WT and CD38-/- mice succesfully(putity>95%), idetified CD38-/- mice, the development of spleen was obstacled, and both the number of spleen cells and B-cells in spleen were significantly decreased in CD38-/- mice(P<0.01), accompanied with the decreasing expression level of TNF-α(P<0.01) and IL-1β(P<0.01), expression level of Sirt1 was significantly increased. Conclusion CD38 gene knockout may inhibits the expression of inflammatory factors(TNF-α and IL-1β) in spleen B-cells by activates Sirt1 pathway, but it's effects in antoimmune diseases need further study.
Objective: To analysis the number of spleen B-cells and the expression level of inflammatory factors(TNF-α and IL-1β) and Sirt1 in spleen B-cells of CD38~(~(-/-)) mice, to explore the effects of CD38 gene knockout on inflammatory factors of Bcells and its' potential mechanisms. Methods Sorting B-cells from spleens of wide type(WT) C57BL/6 and CD38~(-/-) mice individually by negative sorting method with magnetic beads, counting the cell number, and identifing the purity of sorting B-cells by flow cytometry. c DNA was amplified by RT-PCR, and protein was extracted by RIPA. The mRNA expression levels of inflammatory factors and CD38 gene were detected by Real-time PCR, the protein expression levels of CD38 gene, Neo gene and Sirt1 gene were detected by Western-blot. Results We isolated B-cells from both WT and CD38~(-/-) mice succesfully(putity>95%), idetified CD38~(-/-) mice, the development of spleen was obstacled, and both the number of spleen cells and B-cells in spleen were significantly decreased in CD38~(-/-) mice(P<0.01), accompanied with the decreasing expression level of TNF-α(P<0.01) and IL-1β(P<0.01), expression level of Sirt1 was significantly increased. Conclusion CD38 gene knockout may inhibits the expression of inflammatory factors(TNF-α and IL-1β) in spleen B-cells by activates Sirt1 pathway, but it's effects in antoimmune diseases need further study.
Objective: To analysis the number of spleen B-cells and the expression level of inflammatory factors(TNF-α and IL-1β) and Sirt1 in spleen B-cells of CD38~(~(-/-)) mice, to explore the effects of CD38 gene knockout on inflammatory factors of Bcells and its' potential mechanisms. Methods Sorting B-cells from spleens of wide type(WT) C57BL/6 and CD38~(-/-) mice individually by negative sorting method with magnetic beads, counting the cell number, and identifing the purity of sorting B-cells by flow cytometry. c DNA was amplified by RT-PCR, and protein was extracted by RIPA. The mRNA expression levels of inflammatory factors and CD38 gene were detected by Real-time PCR, the protein expression levels of CD38 gene, Neo gene and Sirt1 gene were detected by Western-blot. Results We isolated B-cells from both WT and CD38~(-/-) mice succesfully(putity>95%), idetified CD38~(-/-) mice, the development of spleen was obstacled, and both the number of spleen cells and B-cells in spleen were significantly decreased in CD38~(-/-) mice(P<0.01), accompanied with the decreasing expression level of TNF-α(P<0.01) and IL-1β(P<0.01), expression level of Sirt1 was significantly increased. Conclusion CD38 gene knockout may inhibits the expression of inflammatory factors(TNF-α and IL-1β) in spleen B-cells by activates Sirt1 pathway, but it's effects in antoimmune diseases need further study.
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