TaSYP71,a Qc-SNARE,Contributes to Wheat Resistance Against Puccinia striiformis f.sp.tritici
详细信息 查看官网全文
- 英文论文题名：TaSYP71,a Qc-SNARE,Contributes to Wheat Resistance Against Puccinia striiformis f.sp.tritici
- 论文作者：LIU Min-jie ; PENG Yan ; LI Hua-yi ; DENG Lin ; WANG Xiao-jie ; KANG Zhen-sheng
- 英文论文作者：LIU Min-jie ; PENG Yan ; LI Hua-yi ; DENG Lin ; WANG Xiao-jie ; KANG Zhen-sheng ; State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection ; Northwest A&F University
- 年：2016
- 作者机构：State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection,Northwest A&F University;
- 英文论文关键词：Puccinia striiformis f.sp.tritici ; Wheat ; SNARE ; Virus-induced gene silencing ; Resistance
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S435.121.42
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：51k
- 原文格式：O
- 会议级别：全国
摘要
N-ethylmaleimide-sensitive factor attachment protein receptors(SNAREs) are involved in plant resistance;however,the role of SYP71 in the regulation of plant-pathogen interactions is not well known.In this study,we characterized a plant-specific SNARE in wheat,TaSYP71,which contains a Qc-SNARE domain.Three homologues are localized on chromosome 1AL,1BL and 1DL.Using Agrobacterium-mediated transient expression,TaSYP71 was localized to the plasma membrane in Nicotiana benthamiana.Quantitative real-time PCR assays revealed that TaSYP71 homologues was induced by NaCl,H_2O_2 stress and infection by virulent and avirulent Puccinia striiformis f.sp.tritici(Pst) isolates.Heterologous expression of TaSYP71 in Schizosaccharomyces pombe elevated tolerance to H_2O_2.Meanwhile,H_2O_2 scavenging gene(TaCAT) was down-regulated in TaSYP71 silenced plants treated by H_2O_2 compared to that in control,which indicated that TaSYP71 enhanced tolerance to H_2O_2 stress possibly by influencing the expression of TaCAT to remove the excessive H_2O_2 accumulation.When TaSYP71 homologues were all silenced in wheat by the virus-induced gene silencing system,wheat plants were more susceptible to Pst,with larger infection area and more haustoria number,but the necrotic area of wheat mesophyll cells were larger,one possible explanation that minor contribution of resistance to Pst was insufficient to hinder pathogen extension when TaSYP71 were silenced,and the necrotic area was enlarged accompanied with the pathogen growth.Of course,later cell death could not be excluded.In addition,the expression of pathogenesis-related genes were down-regulated in TaSYP71 silenced wheat plants.These results together suggest that TaSYP71 play a positive role in wheat defence against Pst.
N-ethylmaleimide-sensitive factor attachment protein receptors(SNAREs) are involved in plant resistance;however,the role of SYP71 in the regulation of plant-pathogen interactions is not well known.In this study,we characterized a plant-specific SNARE in wheat,TaSYP71,which contains a Qc-SNARE domain.Three homologues are localized on chromosome 1AL,1BL and 1DL.Using Agrobacterium-mediated transient expression,TaSYP71 was localized to the plasma membrane in Nicotiana benthamiana.Quantitative real-time PCR assays revealed that TaSYP71 homologues was induced by NaCl,H_2O_2 stress and infection by virulent and avirulent Puccinia striiformis f.sp.tritici(Pst) isolates.Heterologous expression of TaSYP71 in Schizosaccharomyces pombe elevated tolerance to H_2O_2.Meanwhile,H_2O_2 scavenging gene(TaCAT) was down-regulated in TaSYP71 silenced plants treated by H_2O_2 compared to that in control,which indicated that TaSYP71 enhanced tolerance to H_2O_2 stress possibly by influencing the expression of TaCAT to remove the excessive H_2O_2 accumulation.When TaSYP71 homologues were all silenced in wheat by the virus-induced gene silencing system,wheat plants were more susceptible to Pst,with larger infection area and more haustoria number,but the necrotic area of wheat mesophyll cells were larger,one possible explanation that minor contribution of resistance to Pst was insufficient to hinder pathogen extension when TaSYP71 were silenced,and the necrotic area was enlarged accompanied with the pathogen growth.Of course,later cell death could not be excluded.In addition,the expression of pathogenesis-related genes were down-regulated in TaSYP71 silenced wheat plants.These results together suggest that TaSYP71 play a positive role in wheat defence against Pst.
N-ethylmaleimide-sensitive factor attachment protein receptors(SNAREs) are involved in plant resistance;however,the role of SYP71 in the regulation of plant-pathogen interactions is not well known.In this study,we characterized a plant-specific SNARE in wheat,TaSYP71,which contains a Qc-SNARE domain.Three homologues are localized on chromosome 1AL,1BL and 1DL.Using Agrobacterium-mediated transient expression,TaSYP71 was localized to the plasma membrane in Nicotiana benthamiana.Quantitative real-time PCR assays revealed that TaSYP71 homologues was induced by NaCl,H_2O_2 stress and infection by virulent and avirulent Puccinia striiformis f.sp.tritici(Pst) isolates.Heterologous expression of TaSYP71 in Schizosaccharomyces pombe elevated tolerance to H_2O_2.Meanwhile,H_2O_2 scavenging gene(TaCAT) was down-regulated in TaSYP71 silenced plants treated by H_2O_2 compared to that in control,which indicated that TaSYP71 enhanced tolerance to H_2O_2 stress possibly by influencing the expression of TaCAT to remove the excessive H_2O_2 accumulation.When TaSYP71 homologues were all silenced in wheat by the virus-induced gene silencing system,wheat plants were more susceptible to Pst,with larger infection area and more haustoria number,but the necrotic area of wheat mesophyll cells were larger,one possible explanation that minor contribution of resistance to Pst was insufficient to hinder pathogen extension when TaSYP71 were silenced,and the necrotic area was enlarged accompanied with the pathogen growth.Of course,later cell death could not be excluded.In addition,the expression of pathogenesis-related genes were down-regulated in TaSYP71 silenced wheat plants.These results together suggest that TaSYP71 play a positive role in wheat defence against Pst.
引文