Patterns of allele specific expression in an elite rice hybrid under different environmental conditions revealed by comparative transcriptional analyses
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- 英文论文题名：Patterns of allele specific expression in an elite rice hybrid under different environmental conditions revealed by comparative transcriptional analyses
- 论文作者：Lin Shao ; Feng Xing ; Conghao Xu ; Qinghua Zhang ; Lingling Chen ; Qifa Zhang
- 英文论文作者：Lin Shao ; Feng Xing ; Conghao Xu ; Qinghua Zhang ; Lingling Chen ; Qifa Zhang ; National Key Laboratory of Crop Genetic Improvement ; Huazhong Agricultural University
- 年：2016
- 作者机构：National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University;
- 英文论文关键词：allele specific expression ; comparative transcriptional analyses ; heterosis
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S511;Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Allele specific expression(ASE),whereby an allele from one parent was preferentially expressed in the hybrid over the allele from the other parent,has been assumed to play a role in heterosis. In this study,we investigated the extent and patterns of ASE by comparing the transcriptomes of Zhenshan 97(ZS97) and Minghui 63(MH63),and their hybrid,which is the indica cross of Shanyou 63,the most widely grown hybrid in China over the past three decades. The hybrid,and the parents were grown under four different conditions(long day/high temperature(LDHT),long day/low temperature(LDLT),short day/high temperature(SDHT) and short day/low temperature(SDLT)). Shoot at seedling stage,flag leaf and panicle at heading stage were sampled by RNA-seq technology. ASE was identified by aligning the RNA-seq reads to the high quality reference genomes of ZS97 and MH63 recently released from our lab. In three tissues(shoot/flag leaf/panicle),1536/2161/2375,1344/3072/2028,1530/1714/2821 and 1297/3380/3517 genes showed differential expression between the hybrid and the parents at LDHT,LDLT,SDHT and SDLT conditions respectively. Moreover,1172/1325/1216,992/1060/1189,1115/1026/1056 and 987/1099/1152 genes exhibited ASE at LDHT,LDLT,SDHT and SDLT conditions respectively. Subsequent analysis of expression regulation of allelic genes shows dynamic patterns affected by developmental stages and growth conditions. Our results suggested that dynamic patterns of ASE may play a role in the manifestation of heterosis.
Allele specific expression(ASE),whereby an allele from one parent was preferentially expressed in the hybrid over the allele from the other parent,has been assumed to play a role in heterosis. In this study,we investigated the extent and patterns of ASE by comparing the transcriptomes of Zhenshan 97(ZS97) and Minghui 63(MH63),and their hybrid,which is the indica cross of Shanyou 63,the most widely grown hybrid in China over the past three decades. The hybrid,and the parents were grown under four different conditions(long day/high temperature(LDHT),long day/low temperature(LDLT),short day/high temperature(SDHT) and short day/low temperature(SDLT)). Shoot at seedling stage,flag leaf and panicle at heading stage were sampled by RNA-seq technology. ASE was identified by aligning the RNA-seq reads to the high quality reference genomes of ZS97 and MH63 recently released from our lab. In three tissues(shoot/flag leaf/panicle),1536/2161/2375,1344/3072/2028,1530/1714/2821 and 1297/3380/3517 genes showed differential expression between the hybrid and the parents at LDHT,LDLT,SDHT and SDLT conditions respectively. Moreover,1172/1325/1216,992/1060/1189,1115/1026/1056 and 987/1099/1152 genes exhibited ASE at LDHT,LDLT,SDHT and SDLT conditions respectively. Subsequent analysis of expression regulation of allelic genes shows dynamic patterns affected by developmental stages and growth conditions. Our results suggested that dynamic patterns of ASE may play a role in the manifestation of heterosis.
Allele specific expression(ASE),whereby an allele from one parent was preferentially expressed in the hybrid over the allele from the other parent,has been assumed to play a role in heterosis. In this study,we investigated the extent and patterns of ASE by comparing the transcriptomes of Zhenshan 97(ZS97) and Minghui 63(MH63),and their hybrid,which is the indica cross of Shanyou 63,the most widely grown hybrid in China over the past three decades. The hybrid,and the parents were grown under four different conditions(long day/high temperature(LDHT),long day/low temperature(LDLT),short day/high temperature(SDHT) and short day/low temperature(SDLT)). Shoot at seedling stage,flag leaf and panicle at heading stage were sampled by RNA-seq technology. ASE was identified by aligning the RNA-seq reads to the high quality reference genomes of ZS97 and MH63 recently released from our lab. In three tissues(shoot/flag leaf/panicle),1536/2161/2375,1344/3072/2028,1530/1714/2821 and 1297/3380/3517 genes showed differential expression between the hybrid and the parents at LDHT,LDLT,SDHT and SDLT conditions respectively. Moreover,1172/1325/1216,992/1060/1189,1115/1026/1056 and 987/1099/1152 genes exhibited ASE at LDHT,LDLT,SDHT and SDLT conditions respectively. Subsequent analysis of expression regulation of allelic genes shows dynamic patterns affected by developmental stages and growth conditions. Our results suggested that dynamic patterns of ASE may play a role in the manifestation of heterosis.
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