Potential role of LvDscam in specific immune response of Litopenaeus vannamei by oral delivery of VP28 using Bacillus subtilis against white spot syndrome virus
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- 英文论文题名：Potential role of LvDscam in specific immune response of Litopenaeus vannamei by oral delivery of VP28 using Bacillus subtilis against white spot syndrome virus
- 论文作者：Linglin Fu ; Taoping Li ; Yanbo Wang
- 英文论文作者：Linglin Fu ; Taoping Li ; Yanbo Wang ; Food Safety Key Laboratory of Zhejiang Province ; School of Food Science and Biotechnology ; Zhejiang Gongshang University
- 年：2016
- 作者机构：Food Safety Key Laboratory of Zhejiang Province,School of Food Science and Biotechnology,Zhejiang Gongshang University;
- 英文论文关键词：White spot syndrome virus ; Litopenaeus vannamei ; Down syndrome cell adhesion molecule(Dscam) ; rVP28-bs ; Specific immune response
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：英文
- 分类号：S945.4
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：72k
- 原文格式：O
- 会议级别：全国
摘要
Envelope protein VP28 has been suggested as a candidate vaccine component to evoke a better protection against white spot syndrome virus(WSSV),a major shrimp pathogen.In our previous papers,we reported that Bacillus subtilis spores harboring VP28(rVP28-bs) can specifically protect shrimp against WSSV by oral delivery.However,the mechanism that supports the production of unique molecules induced by rVP28-bs to trigger specific immune responses against WSSV is originally unknown.It has recently been suggested that Dscam(Down syndrome cell adhesion molecule),a member of the immunoglobulin superfamily(IgSF),plays an essential role in the alternative adaptive immune system of invertebrates.In this study,we compared the diversity of Litopenaeus vannamei Dscam(LvDscam) variable regions and isoform populations by different antigens immunization(rVP28-bs,B.subtilis and Lipopolysaccharide).A total of 13,15 and 11 expressed alternative sequences were identified for N-terminal Ig2,N-terminal Ig3 and the entire Ig7 domain,respectively,which implies that LvDscam can potentially encode at least 2145 isoforms at this experimental condition.More than half of the unique variants(16 out of 22) were found in the Ig2/Ig3 domains.Further analysis of the interaction between VP28 and unique Ig2/Ig3 variants was confirmed by yeast two-hybrid approach.Moreover,we found that the percentage of haemocytes phagocytosing WSSV was significantly higher(p< 0.001) in shrimp injected with control-siRNA(43.8 ± 2.2) than those injected with Dscam-siRNA(11.3 ± 5.4) in the rVP28-bs immunized groups.With Dscam-siRNA injection,the number of survivors significantly decreased(p< 0.001) in the rVP28-bs immunized group after WSSV challenge compared with those with control-siRNA injection.Our data suggested that LvDscam-mediated pathway may be involved in the specific immune response of shrimp against WSSV induced by rVP28-bs.
Envelope protein VP28 has been suggested as a candidate vaccine component to evoke a better protection against white spot syndrome virus(WSSV),a major shrimp pathogen.In our previous papers,we reported that Bacillus subtilis spores harboring VP28(rVP28-bs) can specifically protect shrimp against WSSV by oral delivery.However,the mechanism that supports the production of unique molecules induced by rVP28-bs to trigger specific immune responses against WSSV is originally unknown.It has recently been suggested that Dscam(Down syndrome cell adhesion molecule),a member of the immunoglobulin superfamily(IgSF),plays an essential role in the alternative adaptive immune system of invertebrates.In this study,we compared the diversity of Litopenaeus vannamei Dscam(LvDscam) variable regions and isoform populations by different antigens immunization(rVP28-bs,B.subtilis and Lipopolysaccharide).A total of 13,15 and 11 expressed alternative sequences were identified for N-terminal Ig2,N-terminal Ig3 and the entire Ig7 domain,respectively,which implies that LvDscam can potentially encode at least 2145 isoforms at this experimental condition.More than half of the unique variants(16 out of 22) were found in the Ig2/Ig3 domains.Further analysis of the interaction between VP28 and unique Ig2/Ig3 variants was confirmed by yeast two-hybrid approach.Moreover,we found that the percentage of haemocytes phagocytosing WSSV was significantly higher(p< 0.001) in shrimp injected with control-siRNA(43.8 ± 2.2) than those injected with Dscam-siRNA(11.3 ± 5.4) in the rVP28-bs immunized groups.With Dscam-siRNA injection,the number of survivors significantly decreased(p< 0.001) in the rVP28-bs immunized group after WSSV challenge compared with those with control-siRNA injection.Our data suggested that LvDscam-mediated pathway may be involved in the specific immune response of shrimp against WSSV induced by rVP28-bs.
Envelope protein VP28 has been suggested as a candidate vaccine component to evoke a better protection against white spot syndrome virus(WSSV),a major shrimp pathogen.In our previous papers,we reported that Bacillus subtilis spores harboring VP28(rVP28-bs) can specifically protect shrimp against WSSV by oral delivery.However,the mechanism that supports the production of unique molecules induced by rVP28-bs to trigger specific immune responses against WSSV is originally unknown.It has recently been suggested that Dscam(Down syndrome cell adhesion molecule),a member of the immunoglobulin superfamily(IgSF),plays an essential role in the alternative adaptive immune system of invertebrates.In this study,we compared the diversity of Litopenaeus vannamei Dscam(LvDscam) variable regions and isoform populations by different antigens immunization(rVP28-bs,B.subtilis and Lipopolysaccharide).A total of 13,15 and 11 expressed alternative sequences were identified for N-terminal Ig2,N-terminal Ig3 and the entire Ig7 domain,respectively,which implies that LvDscam can potentially encode at least 2145 isoforms at this experimental condition.More than half of the unique variants(16 out of 22) were found in the Ig2/Ig3 domains.Further analysis of the interaction between VP28 and unique Ig2/Ig3 variants was confirmed by yeast two-hybrid approach.Moreover,we found that the percentage of haemocytes phagocytosing WSSV was significantly higher(p< 0.001) in shrimp injected with control-siRNA(43.8 ± 2.2) than those injected with Dscam-siRNA(11.3 ± 5.4) in the rVP28-bs immunized groups.With Dscam-siRNA injection,the number of survivors significantly decreased(p< 0.001) in the rVP28-bs immunized group after WSSV challenge compared with those with control-siRNA injection.Our data suggested that LvDscam-mediated pathway may be involved in the specific immune response of shrimp against WSSV induced by rVP28-bs.
引文
Amend D.F.(1981)Potency testing of fish vaccines.In:Fish Biologies:Serodiagnostics and Vaccines(ed.by D.P.Anderson&W.Hennessen),pp.447-454.S.Karger AG,Basel.
Anastassiou D.,Liu H.&Varadan V.(2006)Variable window binding for mutually exclusive alternative splicing.Genome Biology 7,R2.
Arts J.A.J.,Taverne-Thiele A.J.,Savelkoul H.F.J.&Rombout J.H.W.M.(2007)Haemocyte reactions in WSSV immersion infected Penaeus monodon.Fish and Shellfish Immunology 23,164-170.
Anastassiou D.,Liu H.&Varadan V.(2006)Variable window binding for mutually exclusive alternative splicing.Genome Biology 7,R2.
Arts J.A.J.,Taverne-Thiele A.J.,Savelkoul H.F.J.&Rombout J.H.W.M.(2007)Haemocyte reactions in WSSV immersion infected Penaeus monodon.Fish and Shellfish Immunology 23,164-170.