The diversity of Vibrio vulnificus cytolysin on the cytotoxicity of murine spleen and lung T cell subsets
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- 英文论文题名：The diversity of Vibrio vulnificus cytolysin on the cytotoxicity of murine spleen and lung T cell subsets
- 论文作者：Gao Hhui ; Zheng Yi ; Xie Danli ; Lou Yongliang
- 英文论文作者：Gao Hhui ; Zheng Yi ; Xie Danli ; Lou Yongliang ; Department of Microbiology and Immunology ; School of Laboratory Medicine ; Wenzhou Medical University
- 年：2016
- 作者机构：Department of Microbiology and Immunology,School of Laboratory Medicine,Wenzhou Medical University;
- 英文论文关键词：vibrio vulnificus hemolysin ; CD4+T cells ; CD8+T cells
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1327k
- 原文格式：D
- 会议级别：全国
摘要
Objective: Vibrio vulnificus is a Gram-negative marine bacterium, which can cause serious septicemia. A present study demonstrated vibrio vulnificus hemolysin plays a important role in this process. However, there is few report about the function of the vibrio vulnificus hemolysin in the adaptive immunity. This study aims to understand how vibrio vulnificus hemolysin(Vvh A) affects the cytotoxicity,viability, tissue variability of murine spleen and lung T cellsubsets.Methods: The murine spleen and lung single cell suspension were isolated from C57BL/6 mice, and then were treated with recombinant Vvh A(r Vvh A) for 6 hours in vitro to determine the viability and tissue variability of murine spleen and lung CD8~+T cells and CD8~+T cells. Results: After treated with recombinant Vvh A(r Vvh A) for 6 hours in vitro, the viability of murine spleen CD8~+T cells and CD8~+T cells was decreased and CD8~+T cells showed more sensitive to the cytotoxicity of r Vvh A, but the viability of murine lung CD8~+T cells and CD8~+T cells was no difference. Furthermore, the murine spleen na?ve CD8~+T cells and effector CD8~+Tcells were also decreased, while there was no change in the lung. Conclusion: The r Vvh A can kill the T cells of spleen and lung in vitro. Besides, the cytotoxicity of r Vvh A to murine spleen and lung T cell subsets showed a differences among tissues, which may have a relationship with na?ve CD8~+Tcells.
Objective: Vibrio vulnificus is a Gram-negative marine bacterium, which can cause serious septicemia. A present study demonstrated vibrio vulnificus hemolysin plays a important role in this process. However, there is few report about the function of the vibrio vulnificus hemolysin in the adaptive immunity. This study aims to understand how vibrio vulnificus hemolysin(Vvh A) affects the cytotoxicity,viability, tissue variability of murine spleen and lung T cellsubsets.Methods: The murine spleen and lung single cell suspension were isolated from C57BL/6 mice, and then were treated with recombinant Vvh A(rVvhA) for 6 hours in vitro to determine the viability and tissue variability of murine spleen and lung CD4~+T cells and CD8~+T cells. Results: After treated with recombinant Vvh A(rVvhA) for 6 hours in vitro, the viability of murine spleen CD4~+T cells and CD8~+T cells was decreased and CD4~+T cells showed more sensitive to the cytotoxicity of rVvhA, but the viability of murine lung CD4~+T cells and CD8~+T cells was no difference. Furthermore, the murine spleen na?ve CD4~+T cells and effector CD4~+Tcells were also decreased, while there was no change in the lung. Conclusion: The rVvhA can kill the T cells of spleen and lung in vitro. Besides, the cytotoxicity of rVvhA to murine spleen and lung T cell subsets showed a differences among tissues, which may have a relationship with na?ve CD4~+Tcells.
Objective: Vibrio vulnificus is a Gram-negative marine bacterium, which can cause serious septicemia. A present study demonstrated vibrio vulnificus hemolysin plays a important role in this process. However, there is few report about the function of the vibrio vulnificus hemolysin in the adaptive immunity. This study aims to understand how vibrio vulnificus hemolysin(Vvh A) affects the cytotoxicity,viability, tissue variability of murine spleen and lung T cellsubsets.Methods: The murine spleen and lung single cell suspension were isolated from C57BL/6 mice, and then were treated with recombinant Vvh A(rVvhA) for 6 hours in vitro to determine the viability and tissue variability of murine spleen and lung CD4~+T cells and CD8~+T cells. Results: After treated with recombinant Vvh A(rVvhA) for 6 hours in vitro, the viability of murine spleen CD4~+T cells and CD8~+T cells was decreased and CD4~+T cells showed more sensitive to the cytotoxicity of rVvhA, but the viability of murine lung CD4~+T cells and CD8~+T cells was no difference. Furthermore, the murine spleen na?ve CD4~+T cells and effector CD4~+Tcells were also decreased, while there was no change in the lung. Conclusion: The rVvhA can kill the T cells of spleen and lung in vitro. Besides, the cytotoxicity of rVvhA to murine spleen and lung T cell subsets showed a differences among tissues, which may have a relationship with na?ve CD4~+Tcells.
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