<正>In this study,we report the cloning of the SsCut gene(GenBank Accession No.XM_001590986.1)encoding cutinase from Sclerotinia sclerotiorum.We isolated a 609-bp cDNA encoding a polypeptide of 202 amino acids with a molecular weight of 20.4 kDa.Heterologous expression of SsCut in Escherichia coli(His-SsCut)caused the formation of lesions in tobacco that closely resembled hypersen-