雌雄同体雌性先熟石斑鱼StAR及3βHSD1基因的克隆鉴定及其受促性腺激素抑制激素调控的研究
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- 英文论文题名：Molecular identification of StAR and 3βHSD1 and characterization in response to GnIH stimulation in protogynous hermaphroditic grouper(Epinephelus coioides)
- 论文作者：刘晓春 ; 姚谧 ; 王晴晴 ; 郭银
- 英文论文作者：Xiaochun Liu ; Mi Yao ; Qingqing Wang ; Yin Guo ; State Key Laboratory of Biocontrol ; Guangdong Province Key Laboratory for Improved Variety Reproduction of Aquatic Economic Animals and Institute of Aquatic Economic Animals ; School of Life Sciences ; Sun Yat-Sen University
- 年：2016
- 作者机构：广东省水生经济动物良种繁育重点实验室生命科学学院中山大学;
- 论文关键词：StAR ; 3βHSD1 ; GnIH ; GnIHR ; 性逆转 ; 斜带石斑鱼
- 英文论文关键词：StAR ; 3βHSD1 ; GnIH ; GnIHR ; Sex reversal ; Epinephelus coioides
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：585k
- 原文格式：D
- 会议级别：全国
摘要
本文克隆得到了斜带石斑鱼3βHSD1和StAR基因cDNA全长。通过氨基酸序列比对和系统进化树分析,确定克隆得到的序列为3βHSD1和StAR基因。对3βHSD1和StAR基因进行组织分布检测,发现这两个基因都主要在性腺中表达。推测3βHSD1和StAR能够在性腺水平,对类固醇合成过程起作用。对于3βHSD1和StAR基因在MT性逆转过程中mRNA水平的表达情况进行了检测。发现3βHSD1和StAR基因mRNA水平在埋植MT过程中性腺的表达与同期对照组比较均呈现显著性升高的趋势,且在兼性(第4周)和雄性(第6周)时,呈现极显著差异。鉴于其对类固醇激素合成的作用,推测3βHSD1和StAR可能参与了斜带石斑鱼MT诱导的性逆转。
Gonadal steroids are critical factors in reproduction and sex reverse process. In the present study, StAR and 3βHSD1 were cloned and characterized from orange-spotted grouper.Moreover, their expression regulated by GnIH was examined. In vitro study showed that treatment of cultured ovary fragments with g GnIH peptides significantly stimulated the expression of StAR and 3βHSD1. In addition, the mRNA levels of StAR and 3βHSD1 were significantly increased after intraperitoneal injection(i.p.) with g GnIH peptides. Moreover, during MT-induced sex change from female to male, the levels of StAR mRNA significantly increased by 5.2, 24.8 and353.5 folds, and that of 3βHSD1 mRNA by 3.5, 32.5 and 55.4 folds at the 2nd, 4th and 6th week after MT implantation, respectively. Collectively, our results indicate that g GnIH may be involved in the regulation of sex reversal or male maintenance by stimulating the expression of StAR and3βHSD1 in protogynous grouper.
Gonadal steroids are critical factors in reproduction and sex reverse process. In the present study, StAR and 3βHSD1 were cloned and characterized from orange-spotted grouper.Moreover, their expression regulated by GnIH was examined. In vitro study showed that treatment of cultured ovary fragments with g GnIH peptides significantly stimulated the expression of StAR and 3βHSD1. In addition, the mRNA levels of StAR and 3βHSD1 were significantly increased after intraperitoneal injection(i.p.) with g GnIH peptides. Moreover, during MT-induced sex change from female to male, the levels of StAR mRNA significantly increased by 5.2, 24.8 and353.5 folds, and that of 3βHSD1 mRNA by 3.5, 32.5 and 55.4 folds at the 2nd, 4th and 6th week after MT implantation, respectively. Collectively, our results indicate that g GnIH may be involved in the regulation of sex reversal or male maintenance by stimulating the expression of StAR and3βHSD1 in protogynous grouper.
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