IL-35 secreted by trophoblasts participates in the regulation of maternal–fetal tolerance in early pregnancy
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- 英文论文题名：IL-35 secreted by trophoblasts participates in the regulation of maternal–fetal tolerance in early pregnancy
- 论文作者：Shengnan Hao ; Xi Chen ; Zhonghua Zhao ; Qianqian Shao ; Ying He ; Wenjuan Gao ; Haiting Mao
- 英文论文作者：Shengnan Hao ; Xi Chen ; Zhonghua Zhao ; Qianqian Shao ; Ying He ; Wenjuan Gao ; Haiting Mao ; Institute of Basic Medicial Sciences ; Qi Lu Hospital ; Shandong University
- 年：2016
- 作者机构：Institute of Basic Medicial Sciences,Qi Lu Hospital,Shandong University;
- 英文论文关键词：IL-35 ; trophoblast cell ; maternal-fetal tolerance ; iTr35
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R714
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1262k
- 原文格式：D
- 会议级别：全国
摘要
Background:Interleukin 35(IL-35) belongs to the IL-12 cytokine family that inhibits T cell proliferation and converts naive T cells into IL-35-producing induced regulatory T cells(i Tr35).Physiological pregnancy requires balence of maternal–fetal tolerance where trophoblasts play essential roles.Objective:We sought to identify the function of IL-35 secreted by trophoblasts in maintain maternal-fetal tolerance during pregnancy.Methods:Human primary trophoblast cells and human trophoblast cell lines,HTR-8/SVneo,were used for ex vivo studies.Human CD4+CD25-T cells isolated from PBMC were cultured with supernatant of primary trophoblast cells or HTR-8/SVneo.ELISA assay and immunocytochemistry was used identify IL-35 level of trophoblast cells.Expression of p35 and EBI3 was quantified using flow cytometry and real-time RT-PCR.Molecules in JAK-STAT pathway were evaluated by western blot method.Results:In this study we showed that first-trimester human trophoblast cells expressed and secreted IL-35.The supernatants from trophoblast cells inhibited directly the proliferation of CD4+ effective T cells(Teffs) by IL-35-dependent manner.Like exogenous IL-35,trophoblasts-drived IL-35 also induced Teffs into i Tr35 with increased expression of p35 and EBI3 at both m RNA and protein levels.Furthermore,we demonstrated that trophoblasts-drived IL-35 played the inhibition function by activating transcription factor STAT1/STAT3.Conclusions:Human trophoblasts contribute to maternal-fetal tolerance by converting the suppressed decidual T cells into i Tr35 via the secretion of IL-35 in human early pregnancy.
Background:Interleukin 35(IL-35) belongs to the IL-12 cytokine family that inhibits T cell proliferation and converts naive T cells into IL-35-producing induced regulatory T cells(i Tr35).Physiological pregnancy requires balence of maternal–fetal tolerance where trophoblasts play essential roles.Objective:We sought to identify the function of IL-35 secreted by trophoblasts in maintain maternal-fetal tolerance during pregnancy.Methods:Human primary trophoblast cells and human trophoblast cell lines,HTR-8/SVneo,were used for ex vivo studies.Human CD4+CD25-T cells isolated from PBMC were cultured with supernatant of primary trophoblast cells or HTR-8/SVneo.ELISA assay and immunocytochemistry was used identify IL-35 level of trophoblast cells.Expression of p35 and EBI3 was quantified using flow cytometry and real-time RT-PCR.Molecules in JAK-STAT pathway were evaluated by western blot method.Results:In this study we showed that first-trimester human trophoblast cells expressed and secreted IL-35.The supernatants from trophoblast cells inhibited directly the proliferation of CD4+ effective T cells(Teffs) by IL-35-dependent manner.Like exogenous IL-35,trophoblasts-drived IL-35 also induced Teffs into i Tr35 with increased expression of p35 and EBI3 at both m RNA and protein levels.Furthermore,we demonstrated that trophoblasts-drived IL-35 played the inhibition function by activating transcription factor STAT1/STAT3.Conclusions:Human trophoblasts contribute to maternal-fetal tolerance by converting the suppressed decidual T cells into i Tr35 via the secretion of IL-35 in human early pregnancy.
Background:Interleukin 35(IL-35) belongs to the IL-12 cytokine family that inhibits T cell proliferation and converts naive T cells into IL-35-producing induced regulatory T cells(i Tr35).Physiological pregnancy requires balence of maternal–fetal tolerance where trophoblasts play essential roles.Objective:We sought to identify the function of IL-35 secreted by trophoblasts in maintain maternal-fetal tolerance during pregnancy.Methods:Human primary trophoblast cells and human trophoblast cell lines,HTR-8/SVneo,were used for ex vivo studies.Human CD4+CD25-T cells isolated from PBMC were cultured with supernatant of primary trophoblast cells or HTR-8/SVneo.ELISA assay and immunocytochemistry was used identify IL-35 level of trophoblast cells.Expression of p35 and EBI3 was quantified using flow cytometry and real-time RT-PCR.Molecules in JAK-STAT pathway were evaluated by western blot method.Results:In this study we showed that first-trimester human trophoblast cells expressed and secreted IL-35.The supernatants from trophoblast cells inhibited directly the proliferation of CD4+ effective T cells(Teffs) by IL-35-dependent manner.Like exogenous IL-35,trophoblasts-drived IL-35 also induced Teffs into i Tr35 with increased expression of p35 and EBI3 at both m RNA and protein levels.Furthermore,we demonstrated that trophoblasts-drived IL-35 played the inhibition function by activating transcription factor STAT1/STAT3.Conclusions:Human trophoblasts contribute to maternal-fetal tolerance by converting the suppressed decidual T cells into i Tr35 via the secretion of IL-35 in human early pregnancy.
引文