Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus
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- 英文论文题名：Two potential recombinant rabies vaccines expressing canine parvovirus virion protein 2 induce immunogenicity to canine parvovirus and rabies virus
- 论文作者：Jun Luo ; Hehe Shi ; Yeping Tan ; Xuefeng Niu ; Teng Long ; Jing Zhao ; Qin Tian ; Yiran Chang ; Yifei Wang ; Hao Chen ; Xiaofeng Guo
- 英文论文作者：Jun Luo ; Hehe Shi ; Yeping Tan ; Xuefeng Niu ; Teng Long ; Jing Zhao ; Qin Tian ; Yiran Chang ; Yifei Wang ; Hao Chen ; Xiaofeng Guo ; College of Veterinary Medicine ; South China Agricultural University ; Key Laboratory of Zoonosis Prevention and Control of Guangdong Province ; Institute of Veterinary Medicine ; Jiangsu Academy of Agricultural Sciences ; Key Laboratory of Veterinary Biologicals engineering and technology ; Ministry of Agriculture ; National Center for Engineering Research of Veterinary Bio-products
- 年：2016
- 作者机构：College of Veterinary Medicine,South China Agricultural University;Key Laboratory of Zoonosis Prevention and Control of Guangdong Province;Institute of Veterinary Medicine,Jiangsu Academy of Agricultural Sciences,Key Laboratory of Veterinary Biologicals engineering and technology,Ministry of Agriculture,National Center for Engineering Research of Veterinary Bio-products;
- 英文论文关键词：Rabies virus ; Canine parvovirus ; VP2 ; Vaccine ; Vector ; HEP-Flury
- 会议召开时间：2016-09-18
- 会议录名称：第六届中国兽药大会论文集
- 英文会议录名称：Proceedings of 2016 the 6th China Congress of Veterinary Medicine
- 语种：英文
- 分类号：S852.65
- 学会代码：ZGXJ
- 会议名称：第六届中国兽药大会
- 会议地点：中国四川成都
- 主办单位：中国兽医药品监察所、中国兽药协会
- 学会名称：中国畜牧兽医学会
- 页数：2
- 文件大小：127k
- 原文格式：O
- 会议级别：全国
摘要
Both rabies virus(RABV) and canine parvovirus(CPV) cause lethal diseases in dogs.In this study,both high egg passage Flury(HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein(G) gene were used to express the CPV virion protein 2(VP2)gene,and were designated rHEP-VP2 and,rHEP-dG-VP2 respectively.The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury.Western blotting indicated that G protein and VP2 were expressed in vitro.The expression of VP2 in Crandell feline kidney cells post-infection by rHEPVP2 and rHEP-dG-VP2 was confirmed by indirect immunofluorescence assay with antibody against VP2.Immunogenicity of recombinant rabies viruses was tested in Kunming mice.Both rHEP-VP2 and rHEP-dG-VP2 induced high levels of rabies antibody compared with HEP-Flury.Mice immunized with rHEP-VP2 and rHEP-dG-VP2 both had a high level of antibodies against VP2,which can protect against CPV infection.A challenge experiment indicated that more than80%mice immunized with recombinant RABVs survived after infection of challenge virus standard 24(CVS-24).Together,this study showed that recombinant RABVs expressing VP2 induced protective immune responses to RABV and CPV.Therefore,rHEP-VP2 and rHEP-dGVP2 might be potential combined vaccines for RABV and CPV.
Both rabies virus(RABV) and canine parvovirus(CPV) cause lethal diseases in dogs.In this study,both high egg passage Flury(HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein(G) gene were used to express the CPV virion protein 2(VP2)gene,and were designated rHEP-VP2 and,rHEP-dG-VP2 respectively.The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury.Western blotting indicated that G protein and VP2 were expressed in vitro.The expression of VP2 in Crandell feline kidney cells post-infection by rHEPVP2 and rHEP-dG-VP2 was confirmed by indirect immunofluorescence assay with antibody against VP2.Immunogenicity of recombinant rabies viruses was tested in Kunming mice.Both rHEP-VP2 and rHEP-dG-VP2 induced high levels of rabies antibody compared with HEP-Flury.Mice immunized with rHEP-VP2 and rHEP-dG-VP2 both had a high level of antibodies against VP2,which can protect against CPV infection.A challenge experiment indicated that more than80%mice immunized with recombinant RABVs survived after infection of challenge virus standard 24(CVS-24).Together,this study showed that recombinant RABVs expressing VP2 induced protective immune responses to RABV and CPV.Therefore,rHEP-VP2 and rHEP-dGVP2 might be potential combined vaccines for RABV and CPV.
Both rabies virus(RABV) and canine parvovirus(CPV) cause lethal diseases in dogs.In this study,both high egg passage Flury(HEP-Flury) strains of RABV and recombinant RABV carrying double RABV glycoprotein(G) gene were used to express the CPV virion protein 2(VP2)gene,and were designated rHEP-VP2 and,rHEP-dG-VP2 respectively.The two recombinant RABVs maintained optimal virus titration according to their viral growth kinetics assay compared with the parental strain HEP-Flury.Western blotting indicated that G protein and VP2 were expressed in vitro.The expression of VP2 in Crandell feline kidney cells post-infection by rHEPVP2 and rHEP-dG-VP2 was confirmed by indirect immunofluorescence assay with antibody against VP2.Immunogenicity of recombinant rabies viruses was tested in Kunming mice.Both rHEP-VP2 and rHEP-dG-VP2 induced high levels of rabies antibody compared with HEP-Flury.Mice immunized with rHEP-VP2 and rHEP-dG-VP2 both had a high level of antibodies against VP2,which can protect against CPV infection.A challenge experiment indicated that more than80%mice immunized with recombinant RABVs survived after infection of challenge virus standard 24(CVS-24).Together,this study showed that recombinant RABVs expressing VP2 induced protective immune responses to RABV and CPV.Therefore,rHEP-VP2 and rHEP-dGVP2 might be potential combined vaccines for RABV and CPV.
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