The subset characteristics of γδT cells in the lung of mice following Chlamydial muridarum airway infection
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- 英文论文题名：The subset characteristics of γδT cells in the lung of mice following Chlamydial muridarum airway infection
- 论文作者：sun lida ; Pang Gaoju ; Wang Yue ; Tang Yingying ; Qiao Sai ; Liang Juyou ; Zhao Huili ; iu Tengli ; Zheng Ningbo ; Tan Lu ; Zhang Yongci ; Zhang Hong ; Bai Hong
- 英文论文作者：sun lida ; Pang Gaoju ; Wang Yue ; Tang Yingying ; Qiao Sai ; Liang Juyou ; Zhao Huili ; iu Tengli ; Zheng Ningbo ; Tan Lu ; Zhang Yongci ; Zhang Hong ; Bai Hong ; Department of Immunology ; School of Basic Medical Sciences ; Tianjin Medical University ; Tianjin Key Laboratory of Cellular and Molecular Immunology ; Key Laboratory of Educational Ministry of China
- 年：2016
- 作者机构：Department of Immunology,School of Basic Medical Sciences,Tianjin Medical University,Tianjin Key Laboratory of Cellular and Molecular Immunology,Key Laboratory of Educational Ministry of China;
- 英文论文关键词：Chlamydia muridarum ; γδT cells subsets ; IL-17 ; IFNγ
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R56
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1362k
- 原文格式：D
- 会议级别：全国
摘要
Our previous studies have shown that γδT cells plays an immune protective role against Chlamydial muridarum(Cm) lung infection by producing INFγ and IL-17 at the early stage of infection. However, the γδT cells subsets and their functions in immune protection against Cm infection remain elusive. Here, We investigate the proliferation, activation and function of lung γδT cells subsets following Cm lung infection.WT mice were inoculated intranasally with 1×10~3 inclusion-forming units(IFUs) of Cm to induce the murine Chlamydia pneumonia. The γδT cells subsets in lung mononuclear cells were analysed by using flow cytometry and RT-PCR, intra-cellular cytokine staining was used to detect IFNγ and IL-17 secretion levels of Vγ1~+T, Vγ4~+T cells. Our results showed that there are 5 kinds of γδT cell subsets including Vγ1~+T、Vγ2~+T、Vγ4~+T、Vγ5~+T and Vγ6~+T cells in lung of mice and the contents are compared to Vγ2 > Vγ4 > Vγ1 > Vγ6 > Vγ5 in WT mice. Remarkably, Cm lung infection mainly induced Vγ4~+T cells proliferation in early stage, and then Vγ1~+T cells became the main γδT cell subsets gradually. Vγ6~+T also increased after infection and proliferate significantly on 7th day, however Vγ2~+T and Vγ5~+T cells had no significant change in the lung after Cm infection. Cytokines detection showed Vγ1~+T cells could secrete IFNγ but do not product IL-17, and Cm infection induced IFNγ secretion reached the peak on 7th day(p<0.01). Vγ4~+T cells could secrete IFNγ and IL-17, and the cytokines levels increased rapidly after Cm infection and reached the peak on 3th day(p<0.001). These findings provide in vivo evidence that Vγ4~+T cells is the major IL-17 and IFNγ-producing γδT cell subsets at the early period of Cm airway infection.
Our previous studies have shown that γδT cells plays an immune protective role against Chlamydial muridarum(Cm) lung infection by producing INFγ and IL-17 at the early stage of infection. However, the γδT cells subsets and their functions in immune protection against Cm infection remain elusive. Here, We investigate the proliferation, activation and function of lung γδT cells subsets following Cm lung infection.WT mice were inoculated intranasally with 1×10~3 inclusion-forming units(IFUs) of Cm to induce the murine Chlamydia pneumonia. The γδT cells subsets in lung mononuclear cells were analysed by using flow cytometry and RT-PCR, intra-cellular cytokine staining was used to detect IFNγ and IL-17 secretion levels of Vγ1~+T, Vγ4~+T cells. Our results showed that there are 5 kinds of γδT cell subsets including Vγ1~+T、Vγ2~+T、Vγ4~+T、Vγ5~+T and Vγ6~+T cells in lung of mice and the contents are compared to Vγ2 > Vγ4 > Vγ1 > Vγ6 > Vγ5 in WT mice. Remarkably, Cm lung infection mainly induced Vγ4~+T cells proliferation in early stage, and then Vγ1~+T cells became the main γδT cell subsets gradually. Vγ6~+T also increased after infection and proliferate significantly on 7th day, however Vγ2~+T and Vγ5~+T cells had no significant change in the lung after Cm infection. Cytokines detection showed Vγ1~+T cells could secrete IFNγ but do not product IL-17, and Cm infection induced IFNγ secretion reached the peak on 7th day(p<0.01). Vγ4~+T cells could secrete IFNγ and IL-17, and the cytokines levels increased rapidly after Cm infection and reached the peak on 3th day(p<0.001). These findings provide in vivo evidence that Vγ4~+T cells is the major IL-17 and IFNγ-producing γδT cell subsets at the early period of Cm airway infection.
Our previous studies have shown that γδT cells plays an immune protective role against Chlamydial muridarum(Cm) lung infection by producing INFγ and IL-17 at the early stage of infection. However, the γδT cells subsets and their functions in immune protection against Cm infection remain elusive. Here, We investigate the proliferation, activation and function of lung γδT cells subsets following Cm lung infection.WT mice were inoculated intranasally with 1×10~3 inclusion-forming units(IFUs) of Cm to induce the murine Chlamydia pneumonia. The γδT cells subsets in lung mononuclear cells were analysed by using flow cytometry and RT-PCR, intra-cellular cytokine staining was used to detect IFNγ and IL-17 secretion levels of Vγ1~+T, Vγ4~+T cells. Our results showed that there are 5 kinds of γδT cell subsets including Vγ1~+T、Vγ2~+T、Vγ4~+T、Vγ5~+T and Vγ6~+T cells in lung of mice and the contents are compared to Vγ2 > Vγ4 > Vγ1 > Vγ6 > Vγ5 in WT mice. Remarkably, Cm lung infection mainly induced Vγ4~+T cells proliferation in early stage, and then Vγ1~+T cells became the main γδT cell subsets gradually. Vγ6~+T also increased after infection and proliferate significantly on 7th day, however Vγ2~+T and Vγ5~+T cells had no significant change in the lung after Cm infection. Cytokines detection showed Vγ1~+T cells could secrete IFNγ but do not product IL-17, and Cm infection induced IFNγ secretion reached the peak on 7th day(p<0.01). Vγ4~+T cells could secrete IFNγ and IL-17, and the cytokines levels increased rapidly after Cm infection and reached the peak on 3th day(p<0.001). These findings provide in vivo evidence that Vγ4~+T cells is the major IL-17 and IFNγ-producing γδT cell subsets at the early period of Cm airway infection.
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