The Signal Peptide-like Segment Affects HpaXm Transport,but without the Pathogenicity of Xanthomonas citri subsp.malvacearum
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- 英文论文题名：The Signal Peptide-like Segment Affects HpaXm Transport,but without the Pathogenicity of Xanthomonas citri subsp.malvacearum
- 论文作者：LIU Yue ; MIAO Wei-guo ; ZHENG Fu-cong
- 英文论文作者：LIU Yue ; MIAO Wei-guo ; ZHENG Fu-cong ; Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection ; Hainan University
- 年：2016
- 作者机构：Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource/College of Environment and Plant Protection,Hainan University;
- 英文论文关键词：HpaXm ; Suspected signal peptide ; HR reaction ; Defensive response ; Immune colloidal gold position
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S436.66
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：62k
- 原文格式：O
- 会议级别：全国
摘要
HpaXm,encoded by the hpaXm gene of Xanthomonas citri subsp.malvacearum(Xcm),is a novel harpin protein described from cotton leaf blight bacteria.We predicted that the N-terminal leader peptide(1- MNSLNTQIGANSSFL- 15) was a putative signal peptide of hpaXm,and determined here whether this segment are related to HpaXm transport or the pathogenicity of the pathogen Xcm.First,transgenic tobacco lines expressing the full-length hpaXm and the signal peptide-like segment-deleted mutant hpaXm△LP were developed using transformation mediated by Agrobacterium tumefaciens.Then the extracted soluble-protein activity was determined.Additionally the defensive responses and resistance to tobacco mosaic virus(TMV) of transgenic tobacco were identified.Finally,differences in the association sites between the endogenously expressed hpaXm and hpaXm△LP in transgenic tobacco tissues were demonstrated.Molecular identification showed that the target genes had already integrated into the recipient genomes of transgenic tobacco respectively and were expressed normally.Soluble proteins extracted from plants transformed with hpaXm and hpaXm△LP were bio-active.Defensive responses of hpaXm and hpaXm△LP as micro-HR were observed on transgenic tobacco leaves.Disease resistance bioassays showed that tobacco plants transformed with hpaXm had slightly enhanced resistance to TMV compared to those transformed with hpaXm△LP.Immune colloidal-gold detection showed that the hpaXm expressed in transgenic tobaccos was transferred to the plasma membrane and the cell wall of tobacco,but in the deletion mutant hpaXm△LP expressed in transgenic tobacco could not cross the membrane to reach the cell wall.In summary,the N-terminal signal peptide-like fragment(1 ~45bp) in HpaXm sequence was not necessary for endogenous expression and bioactivity of HpaXm in transgenic tobacco and the pathogenicity of Xanthomonas citri subsp.malvacearum.But in the deletion mutant it prevented hpaXm△LP being transferred to the cell wall,thus slightly reducing its resistance to TMV.
HpaXm,encoded by the hpaXm gene of Xanthomonas citri subsp.malvacearum(Xcm),is a novel harpin protein described from cotton leaf blight bacteria.We predicted that the N-terminal leader peptide(1- MNSLNTQIGANSSFL- 15) was a putative signal peptide of hpaXm,and determined here whether this segment are related to HpaXm transport or the pathogenicity of the pathogen Xcm.First,transgenic tobacco lines expressing the full-length hpaXm and the signal peptide-like segment-deleted mutant hpaXm△LP were developed using transformation mediated by Agrobacterium tumefaciens.Then the extracted soluble-protein activity was determined.Additionally the defensive responses and resistance to tobacco mosaic virus(TMV) of transgenic tobacco were identified.Finally,differences in the association sites between the endogenously expressed hpaXm and hpaXm△LP in transgenic tobacco tissues were demonstrated.Molecular identification showed that the target genes had already integrated into the recipient genomes of transgenic tobacco respectively and were expressed normally.Soluble proteins extracted from plants transformed with hpaXm and hpaXm△LP were bio-active.Defensive responses of hpaXm and hpaXm△LP as micro-HR were observed on transgenic tobacco leaves.Disease resistance bioassays showed that tobacco plants transformed with hpaXm had slightly enhanced resistance to TMV compared to those transformed with hpaXm△LP.Immune colloidal-gold detection showed that the hpaXm expressed in transgenic tobaccos was transferred to the plasma membrane and the cell wall of tobacco,but in the deletion mutant hpaXm△LP expressed in transgenic tobacco could not cross the membrane to reach the cell wall.In summary,the N-terminal signal peptide-like fragment(1 ~45bp) in HpaXm sequence was not necessary for endogenous expression and bioactivity of HpaXm in transgenic tobacco and the pathogenicity of Xanthomonas citri subsp.malvacearum.But in the deletion mutant it prevented hpaXm△LP being transferred to the cell wall,thus slightly reducing its resistance to TMV.
HpaXm,encoded by the hpaXm gene of Xanthomonas citri subsp.malvacearum(Xcm),is a novel harpin protein described from cotton leaf blight bacteria.We predicted that the N-terminal leader peptide(1- MNSLNTQIGANSSFL- 15) was a putative signal peptide of hpaXm,and determined here whether this segment are related to HpaXm transport or the pathogenicity of the pathogen Xcm.First,transgenic tobacco lines expressing the full-length hpaXm and the signal peptide-like segment-deleted mutant hpaXm△LP were developed using transformation mediated by Agrobacterium tumefaciens.Then the extracted soluble-protein activity was determined.Additionally the defensive responses and resistance to tobacco mosaic virus(TMV) of transgenic tobacco were identified.Finally,differences in the association sites between the endogenously expressed hpaXm and hpaXm△LP in transgenic tobacco tissues were demonstrated.Molecular identification showed that the target genes had already integrated into the recipient genomes of transgenic tobacco respectively and were expressed normally.Soluble proteins extracted from plants transformed with hpaXm and hpaXm△LP were bio-active.Defensive responses of hpaXm and hpaXm△LP as micro-HR were observed on transgenic tobacco leaves.Disease resistance bioassays showed that tobacco plants transformed with hpaXm had slightly enhanced resistance to TMV compared to those transformed with hpaXm△LP.Immune colloidal-gold detection showed that the hpaXm expressed in transgenic tobaccos was transferred to the plasma membrane and the cell wall of tobacco,but in the deletion mutant hpaXm△LP expressed in transgenic tobacco could not cross the membrane to reach the cell wall.In summary,the N-terminal signal peptide-like fragment(1 ~45bp) in HpaXm sequence was not necessary for endogenous expression and bioactivity of HpaXm in transgenic tobacco and the pathogenicity of Xanthomonas citri subsp.malvacearum.But in the deletion mutant it prevented hpaXm△LP being transferred to the cell wall,thus slightly reducing its resistance to TMV.
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