Differential regulation of clathrin and its adaptor proteins, AP-2 and the TPLATE complex, during their membrane recruitment in Arabidopsis
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- 英文论文题名：Differential regulation of clathrin and its adaptor proteins, AP-2 and the TPLATE complex, during their membrane recruitment in Arabidopsis
- 论文作者：Chao Wang ; Tianwei Hu ; Xu Yan ; Tingting Meng ; Yutong Wang ; Qingmei Wang ; Xiaoyue Zhang ; Ying Gu ; Clara Sánchez-Rodríguez ; Astrid Gadeyne ; Jinxing Lin ; Staffan Persson ; Dani?l Van Damme ; Chuanyou Li ; Sebastian Y.Bednarek ; Jianwei Pan
- 英文论文作者：Chao Wang ; Tianwei Hu ; Xu Yan ; Tingting Meng ; Yutong Wang ; Qingmei Wang ; Xiaoyue Zhang ; Ying Gu ; Clara Sánchez-Rodríguez ; Astrid Gadeyne ; Jinxing Lin ; Staffan Persson ; Dani?l Van Damme ; Chuanyou Li ; Sebastian Y.Bednarek ; Jianwei Pan ; College of Chemistry and Life Sciences ; Zhejiang Normal University ; Institute of Genetics and Developmental Biology ; Chinese Academy of Sciences ; Department of Biochemistry and Molecular Biology ; Pennsylvania State University ; University Park ; Department of Biology ; Institute of Agricultural Sciences ; ETH Zurich ; Department of Plant Systems Biology ; VIB ; Department of Plant Biotechnology and Bioinformatics ; Ghent University ; College of Biological Sciences and Biotechnology ; Beijing Forestry University ; ARC Centre of Excellence in Plant Cell Walls ; School of Biosciences ; University of Melbourne ; Department of Biochemistry ; University of Wisconsin
- 年：2016
- 作者机构：College of Chemistry and Life Sciences,Zhejiang Normal University;Institute of Genetics and Developmental Biology,Chinese Academy of Sciences;Department of Biochemistry and Molecular Biology,Pennsylvania State University,University Park;Department of Biology,Institute of Agricultural Sciences,ETH Zurich;Department of Plant Systems Biology,VIB,Department of Plant Biotechnology and Bioinformatics,Ghent University;College of Biological Sciences and Biotechnology,Beijing Forestry University;ARC Centre of Excellence in Plant Cell Walls,School of Biosciences,University of Melbourne;Department of Biochemistry,University of Wisconsin;
- 英文论文关键词：Adaptor Protein 2 ; auxin ; clathrin ; endocytosis ; salicylic acid ; TPLATE complex ; tyrphostin A23 ; Arabidopsis
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q945
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：853k
- 原文格式：D
- 会议级别：全国
摘要
In plants,clathrin-mediated endocytosis(CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes,Adaptor Protein 2(AP-2) and the TPLATE complex(TPC),and is negatively regulated by the hormones auxin and salicylic acid(SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane(PM) to regulate CME are however poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23(Tyr A23) reduce the membrane association of clathrin and AP-2,but not that of the TPC,whereas auxin solely affected clathrin membrane association,in Arabidopsis thaliana. Genetic and pharmacological experiments revealed that loss of AP2μ or AP2σ partially affected the membrane association of other AP-2 subunits and that the AP-2 subunit AP2σ,but not AP2μ,was required for SA-and Tyr A23-dependent inhibition of CME. Furthermore,we show that although AP-2 and the TPC are both required for the PM recruitment of clathrin in wild-type cells,the TPC is necessary for clathrin PM association in AP-2 deficient cells. These results indicate that developmental signals may differentially modulate the membrane recruitment of clathrin and its core accessory complexes to regulate the process of CME in plant cells.
In plants,clathrin-mediated endocytosis(CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes,Adaptor Protein 2(AP-2) and the TPLATE complex(TPC),and is negatively regulated by the hormones auxin and salicylic acid(SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane(PM) to regulate CME are however poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23(Tyr A23) reduce the membrane association of clathrin and AP-2,but not that of the TPC,whereas auxin solely affected clathrin membrane association,in Arabidopsis thaliana. Genetic and pharmacological experiments revealed that loss of AP2μ or AP2σ partially affected the membrane association of other AP-2 subunits and that the AP-2 subunit AP2σ,but not AP2μ,was required for SA-and Tyr A23-dependent inhibition of CME. Furthermore,we show that although AP-2 and the TPC are both required for the PM recruitment of clathrin in wild-type cells,the TPC is necessary for clathrin PM association in AP-2 deficient cells. These results indicate that developmental signals may differentially modulate the membrane recruitment of clathrin and its core accessory complexes to regulate the process of CME in plant cells.
In plants,clathrin-mediated endocytosis(CME) is dependent on the function of clathrin and its accessory heterooligomeric adaptor protein complexes,Adaptor Protein 2(AP-2) and the TPLATE complex(TPC),and is negatively regulated by the hormones auxin and salicylic acid(SA). The details for how clathrin and its adaptor complexes are recruited to the plasma membrane(PM) to regulate CME are however poorly understood. We found that SA and the pharmacological CME inhibitor tyrphostin A23(Tyr A23) reduce the membrane association of clathrin and AP-2,but not that of the TPC,whereas auxin solely affected clathrin membrane association,in Arabidopsis thaliana. Genetic and pharmacological experiments revealed that loss of AP2μ or AP2σ partially affected the membrane association of other AP-2 subunits and that the AP-2 subunit AP2σ,but not AP2μ,was required for SA-and Tyr A23-dependent inhibition of CME. Furthermore,we show that although AP-2 and the TPC are both required for the PM recruitment of clathrin in wild-type cells,the TPC is necessary for clathrin PM association in AP-2 deficient cells. These results indicate that developmental signals may differentially modulate the membrane recruitment of clathrin and its core accessory complexes to regulate the process of CME in plant cells.
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