Experimental Trichinella spiralis infection in Hezuo Pig and immune response analysis
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- 英文论文题名：Experimental Trichinella spiralis infection in Hezuo Pig and immune response analysis
- 论文作者：Tingting Li ; Nianzhang Zhang ; Wenhui Li ; Li Li ; Jianmin Cui ; Hongbin Yan ; Zigang Qu ; Yangyang ; Wanzhong Jia ; Baoquan Fu
- 英文论文作者：Tingting Li ; Nianzhang Zhang ; Wenhui Li ; Li Li ; Jianmin Cui ; Hongbin Yan ; Zigang Qu ; Yangyang ; Wanzhong Jia ; Baoquan Fu ; State Key Laboratory of Veterinary Etiological Biology ; Key Laboratory of Veterinary Parasitology of Gansu Province ; Lanzhou Veterinary Research Institute ; Chinese Academy of Agricultural Sciences ; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease
- 年：2016
- 作者机构：State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Disease;
- 英文论文关键词：Trichinella spiralis ; experimental infection ; Hezuo pig ; immunoresponse
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：S858.28
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：156k
- 原文格式：D
- 会议级别：全国
摘要
Trichinellosis caused by Trichinella spiralis is a serious foodborne parasitic zoonosis. Pork and other animal meat are identified as the most important source of infection for humans. Hezuo pig is a sort of primitive local pig, which is from natural domestication and artificial selection. The plateau such as Gannan Tibetan Autonomous Prefecture of Gansu Province, is the main distribution area of Hezuo pig. Because of the free-range systems, Hezuo pig is liable to be attacked by Trichinella spiralis. Furthermore, the pork from Hezuo pig as one of the main source of meat, the residents can be infected by Trichinella spiralis because of the altitude environment and their dietary habit. The aim of the present work is to study the infectivity of Trichinella spiralis Tibet strain to Hezuo pig and the changes of cytokine levels in sera. A total of 9 Hezuo pigs were divided into four groups, including three experimental groups(two pigs in each group) and one control group(three pigs). The pigs in the experimental groups were inoculated with 200, 2000 or 20,000 muscle larvae of Trichinella isolate from Tibet respectively. During the 107 days post-infection, the blood samples were collected at days 0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 100 and 107 post-infection, and the sera were isolated to evaluate the anti-Trichinella Ig G kinetics by ELISA using excretory/secretory antigens and the cytokine levels by the microarray. The results of ELISA showed that the level of antibodies was corresponded with the inoculum dose before the days 56 post-infection, and the pigs receiving high dosage of larvae obtained higher level of antibodies than that receiving low dosage of larvae. The level of antibodies of the pigs receiving 2000 and 20,000 muscle larvae increased sharply after day 21 post-infection, while the pigs receiving 200 larvae increased after day 28 post-infection. Ten cytokines were detected by microarray in the serum, which includes four Th1 cytokines(IFN-γ, IL-1β, IL-8, IL-12), three Th2 cytokines(IL-4, IL-6, IL-10), two Th1/2 cytokines(GM-CSF, TNF-α), one Th3 cytokines(TGF-β1). All the cytokines were detectable in all the samples. Of all the cytokines, IL-12 demonstrated the highest concentration compared with other cytokines and peaked on day 42 post-infection. The four kinds of cytokines had a low concentration in the early period of infection. But after the day 42 post-infection, almost all the cytokines maintained in a high level. At necropsy, muscles from eight organs including diaphragm, tongue, masster, intercostal, psoas, gluteus, foreleg and hind leg were digested artificially to evaluate the muscle larvae burden. There was a positive correlation between the infective dose and the mean larval recovery rate, and the diaphragm and tongue were identified as predilection muscles. The experimental pigs receiving 20,000 larvae showed a maximum of 315 lpg in diaphragm and a minimum of 52 lpg in intercostal, while the pigs receiving 200 larvae showed a maximum of 10 lpg in diaphragm and a minimum of 1 lpg in intercostal. It is the first record of experimental infection in Hezuo pig with Trichinella spiralis Tibet strain.
Trichinellosis caused by Trichinella spiralis is a serious foodborne parasitic zoonosis. Pork and other animal meat are identified as the most important source of infection for humans. Hezuo pig is a sort of primitive local pig, which is from natural domestication and artificial selection. The plateau such as Gannan Tibetan Autonomous Prefecture of Gansu Province, is the main distribution area of Hezuo pig. Because of the free-range systems, Hezuo pig is liable to be attacked by Trichinella spiralis. Furthermore, the pork from Hezuo pig as one of the main source of meat, the residents can be infected by Trichinella spiralis because of the altitude environment and their dietary habit. The aim of the present work is to study the infectivity of Trichinella spiralis Tibet strain to Hezuo pig and the changes of cytokine levels in sera. A total of 9 Hezuo pigs were divided into four groups, including three experimental groups(two pigs in each group) and one control group(three pigs). The pigs in the experimental groups were inoculated with 200, 2000 or 20,000 muscle larvae of Trichinella isolate from Tibet respectively. During the 107 days post-infection, the blood samples were collected at days 0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 100 and 107 post-infection, and the sera were isolated to evaluate the anti-Trichinella Ig G kinetics by ELISA using excretory/secretory antigens and the cytokine levels by the microarray. The results of ELISA showed that the level of antibodies was corresponded with the inoculum dose before the days 56 post-infection, and the pigs receiving high dosage of larvae obtained higher level of antibodies than that receiving low dosage of larvae. The level of antibodies of the pigs receiving 2000 and 20,000 muscle larvae increased sharply after day 21 post-infection, while the pigs receiving 200 larvae increased after day 28 post-infection. Ten cytokines were detected by microarray in the serum, which includes four Th1 cytokines(IFN-γ, IL-1β, IL-8, IL-12), three Th2 cytokines(IL-4, IL-6, IL-10), two Th1/2 cytokines(GM-CSF, TNF-α), one Th3 cytokines(TGF-β1). All the cytokines were detectable in all the samples. Of all the cytokines, IL-12 demonstrated the highest concentration compared with other cytokines and peaked on day 42 post-infection. The four kinds of cytokines had a low concentration in the early period of infection. But after the day 42 post-infection, almost all the cytokines maintained in a high level. At necropsy, muscles from eight organs including diaphragm, tongue, masster, intercostal, psoas, gluteus, foreleg and hind leg were digested artificially to evaluate the muscle larvae burden. There was a positive correlation between the infective dose and the mean larval recovery rate, and the diaphragm and tongue were identified as predilection muscles. The experimental pigs receiving 20,000 larvae showed a maximum of 315 lpg in diaphragm and a minimum of 52 lpg in intercostal, while the pigs receiving 200 larvae showed a maximum of 10 lpg in diaphragm and a minimum of 1 lpg in intercostal. It is the first record of experimental infection in Hezuo pig with Trichinella spiralis Tibet strain.
Trichinellosis caused by Trichinella spiralis is a serious foodborne parasitic zoonosis. Pork and other animal meat are identified as the most important source of infection for humans. Hezuo pig is a sort of primitive local pig, which is from natural domestication and artificial selection. The plateau such as Gannan Tibetan Autonomous Prefecture of Gansu Province, is the main distribution area of Hezuo pig. Because of the free-range systems, Hezuo pig is liable to be attacked by Trichinella spiralis. Furthermore, the pork from Hezuo pig as one of the main source of meat, the residents can be infected by Trichinella spiralis because of the altitude environment and their dietary habit. The aim of the present work is to study the infectivity of Trichinella spiralis Tibet strain to Hezuo pig and the changes of cytokine levels in sera. A total of 9 Hezuo pigs were divided into four groups, including three experimental groups(two pigs in each group) and one control group(three pigs). The pigs in the experimental groups were inoculated with 200, 2000 or 20,000 muscle larvae of Trichinella isolate from Tibet respectively. During the 107 days post-infection, the blood samples were collected at days 0, 7, 14, 21, 28, 35, 42, 49, 56, 63, 70, 100 and 107 post-infection, and the sera were isolated to evaluate the anti-Trichinella Ig G kinetics by ELISA using excretory/secretory antigens and the cytokine levels by the microarray. The results of ELISA showed that the level of antibodies was corresponded with the inoculum dose before the days 56 post-infection, and the pigs receiving high dosage of larvae obtained higher level of antibodies than that receiving low dosage of larvae. The level of antibodies of the pigs receiving 2000 and 20,000 muscle larvae increased sharply after day 21 post-infection, while the pigs receiving 200 larvae increased after day 28 post-infection. Ten cytokines were detected by microarray in the serum, which includes four Th1 cytokines(IFN-γ, IL-1β, IL-8, IL-12), three Th2 cytokines(IL-4, IL-6, IL-10), two Th1/2 cytokines(GM-CSF, TNF-α), one Th3 cytokines(TGF-β1). All the cytokines were detectable in all the samples. Of all the cytokines, IL-12 demonstrated the highest concentration compared with other cytokines and peaked on day 42 post-infection. The four kinds of cytokines had a low concentration in the early period of infection. But after the day 42 post-infection, almost all the cytokines maintained in a high level. At necropsy, muscles from eight organs including diaphragm, tongue, masster, intercostal, psoas, gluteus, foreleg and hind leg were digested artificially to evaluate the muscle larvae burden. There was a positive correlation between the infective dose and the mean larval recovery rate, and the diaphragm and tongue were identified as predilection muscles. The experimental pigs receiving 20,000 larvae showed a maximum of 315 lpg in diaphragm and a minimum of 52 lpg in intercostal, while the pigs receiving 200 larvae showed a maximum of 10 lpg in diaphragm and a minimum of 1 lpg in intercostal. It is the first record of experimental infection in Hezuo pig with Trichinella spiralis Tibet strain.
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