Inhibitory Activity of Maize Root Exudates against Phytophthora nicotianae and Antifungal Compounds Analysis
详细信息 查看官网全文
- 英文论文题名：Inhibitory Activity of Maize Root Exudates against Phytophthora nicotianae and Antifungal Compounds Analysis
- 论文作者：ZHANG Limeng ; JI Sigui ; JIAO Yongge ; LI Jiangzhou ; GU Xinghui ; CUI Yonghe ; ZHAO Jinlong ; ZHU Shusheng ; YANG Min
- 英文论文作者：ZHANG Limeng ; JI Sigui ; JIAO Yongge ; LI Jiangzhou ; GU Xinghui ; CUI Yonghe ; ZHAO Jinlong ; ZHU Shusheng ; YANG Min ; Yunnan Tobacco Company ; Yuxi Branch ; Key Laboratory for Agro‐biodiversity and Pest Control of Ministry of Education ; Yunnan Agricultural University
- 年：2016
- 作者机构：Yunnan Tobacco Company,Yuxi Branch;Key Laboratory for Agro‐biodiversity and Pest Control of Ministry of Education, Yunnan Agricultural University;
- 英文论文关键词：Maize ; Root exudates ; Phytophthora nicotianae ; DIMBOA ; MBOA
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：英文
- 分类号：S513;S572
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：11
- 文件大小：856k
- 原文格式：D
摘要
In this study, we observed the interaction between maize root and zoospores of Phytophthora nicotianae, the inhibitory activity of maize root exudates against P. nicotianae and the antifungal compounds in the maize root exudates were tested and analyzed using HPLC-MS. The results indicated that maize root could not only attract zoospores into the rhizosphere, but also secret some substances to inhibit the swimming of zoospores or cause the rupture of cystospores. Further studies indicated that maize root exudates showed inhibitory activity against the release of zoospores from sporangia, the germination of cycstospores, and the hyphal growth. The inhibition rate was 73.8%, 87.2% and 55.2%, respectively, when the root exudates concentration was 0.90 mg/m L. DIMBOA, 2,4-dihydroxy- 7-methoxy-2H-1,4-benzoxazin-3(4H)-one and its degradation substance MBOA(6-methoxy-2-benzoxazolinone) were identified in root exudates by HPLC-MS. MBOA also showed antifungal activity against the hyphal growth of P. nicotianae. The inhibition ratio reached 90.94% when the concentration of MBOA in media was 0.30 mg/m L. In summary, maize root can not only attract the zoospores of P. nicotianae, but also secret the antifungal compounds DIMBOA and MBOA. These results support maize and tobacco crop rotation as a theoretical control method for tobacco black shank in tobacco growing.
In this study, we observed the interaction between maize root and zoospores of Phytophthora nicotianae, the inhibitory activity of maize root exudates against P. nicotianae and the antifungal compounds in the maize root exudates were tested and analyzed using HPLC-MS. The results indicated that maize root could not only attract zoospores into the rhizosphere, but also secret some substances to inhibit the swimming of zoospores or cause the rupture of cystospores. Further studies indicated that maize root exudates showed inhibitory activity against the release of zoospores from sporangia, the germination of cycstospores, and the hyphal growth. The inhibition rate was 73.8%, 87.2% and 55.2%, respectively, when the root exudates concentration was 0.90 mg/m L. DIMBOA, 2,4-dihydroxy- 7-methoxy-2H-1,4-benzoxazin-3(4H)-one and its degradation substance MBOA(6-methoxy-2-benzoxazolinone) were identified in root exudates by HPLC-MS. MBOA also showed antifungal activity against the hyphal growth of P. nicotianae. The inhibition ratio reached 90.94% when the concentration of MBOA in media was 0.30 mg/m L. In summary, maize root can not only attract the zoospores of P. nicotianae, but also secret the antifungal compounds DIMBOA and MBOA. These results support maize and tobacco crop rotation as a theoretical control method for tobacco black shank in tobacco growing.
In this study, we observed the interaction between maize root and zoospores of Phytophthora nicotianae, the inhibitory activity of maize root exudates against P. nicotianae and the antifungal compounds in the maize root exudates were tested and analyzed using HPLC-MS. The results indicated that maize root could not only attract zoospores into the rhizosphere, but also secret some substances to inhibit the swimming of zoospores or cause the rupture of cystospores. Further studies indicated that maize root exudates showed inhibitory activity against the release of zoospores from sporangia, the germination of cycstospores, and the hyphal growth. The inhibition rate was 73.8%, 87.2% and 55.2%, respectively, when the root exudates concentration was 0.90 mg/m L. DIMBOA, 2,4-dihydroxy- 7-methoxy-2H-1,4-benzoxazin-3(4H)-one and its degradation substance MBOA(6-methoxy-2-benzoxazolinone) were identified in root exudates by HPLC-MS. MBOA also showed antifungal activity against the hyphal growth of P. nicotianae. The inhibition ratio reached 90.94% when the concentration of MBOA in media was 0.30 mg/m L. In summary, maize root can not only attract the zoospores of P. nicotianae, but also secret the antifungal compounds DIMBOA and MBOA. These results support maize and tobacco crop rotation as a theoretical control method for tobacco black shank in tobacco growing.
引文
1 Ahmad S,Veyrat N,Gordon-Weeks R,Zhang Y,Martin J,et al.(2011)Benzoxazinoid metabolites regulate innate immunity against aphids and fungi in maize.Plant Physiology157,317-327.
2 Bais HP,Park SW,Weir TL,Callaway RM,Vivanco JM.2004.How plants communicate using the underground information superhighway.Trends Plant Sci.9:26-32
3 Bais HP,Weir TL,Perry LG,Gilroy S,Vivanco JM.The role of root exudates in rhizosphere interactions with plants and other.Annu.Rev.Plant Biol.2006.57:233-266
4 Bjostard LB,Hibbard BE(1992)6-Methoxy-2-benzoxazotinone:A semiochemical for host location by Western corn root worm 1arvae.Journal of Chemical Ecology 18,931-944.
5 Bouws H,Finckh MR(2008)Effects of strip intercropping of potatoes with non-hosts on late blight severity and tuber yield in organic production.Plant Pathology 57,916-927.
6 Cameron JN,Carlile MJ(1978)Fatty acids,aldehydes and alcohols as attractants for zoospores of Phytophthora palmivora.Nature 271,448-449.
7 Carlile MJ(1983)Motility,taxis and tropism in Phytophthora.In Phytophthora:Its Biology,Taxonomy,Ecology and Pathology,Ed.by D.C.Erwin,S.Bartnicki-Garcia&P H Tsao,pp.95-107,American Phytopathological Society,St.Paul,Minnesota.
8 Cohen Y,Baider A,Cohen BH(1995)Dimethomorph activity against Oomycete fungal plant pathogens,Phytopathology,85,1500-1506.
9 Deacon JW(1988)Behavioural responses of fungal zoospores.Microbiological Sciences 5,249-252.
10 Erb M.,K?llner T.G.,Degenhardt J.,Zwahlen C.,Hibbard B.E.,and Turlings T.C.(2011).The role of abscisic acid and water stress in root herbivore-induced leaf resistance.New Phytol.189,308-320.doi:10.1111/j.1469-8137.2010.03450.x
11 Erwin DC,Ribeiro OK(1996)Phytophthora capsici.In:Erwin D.C.,Ribeiro O.K.(ed),Phytophthora Diseases Worldwide,pp.262-268.APS Press,St.Paul,MN,USA.
12 Erwin DC,Ribeiro OK(1996)Phytophthora capsici.In:Erwin D.C.,Ribeiro O.K.(ed),Phytophthora Diseases Worldwide,pp.262-268.APS Press,St.Paul,MN,USA.
13 Etzerodt T.,Nielsen S.T.,Mortensen A.G.,Christophersen C.,and Fomsgaard I.S.(2006).Elucidating the transformation pattern of the cereal allelochemical 6-methoxy-2-benzoxazolinone(MBOA)and the trideuteriomethoxy analogue[D3]-MBOAin soil.J.Agr.Food Chem.54,1075-1085.doi:10.1021/jf0509052
14 Fan KW,Vrijmoed LL,Jones EBG(2002)Zoospore chemotaxis of Mangrove thraustochytrids from Hong Kong.Mycologia 94,569-578.
15 Friebe A.,Vilich V.,Hennig L.,Kluge M.,and Sicker D.(1998).Detoxification of benzoxazolinone allelochemicals from wheat by Gaeumannomyces graminis var.tritici,G.graminis var.graminis,G.graminis var.avenae,and Fusarium culmorum.Appli.Environ.Microbiol.64,2386-2391.
16 He X,Zhu S,Wang H,Xie Y,Sun Y,et al.(2010)Crop diversity for disease ecological control in potato and maize.Journal of Resources and Ecology 1,45-50.
17 Kumar P,Gagliardo RW,Chilton WS(1993)Soil transformation of wheat and corn metabolites MBOA and DIMBOA into aminophenoxazinones.Journal of Chemical Ecology19,2453-2461.
18 Li T,Wang B,Wang S.1996.The current situations,problems and measures of tobacco rotation in Yunnan.Chinese Tobacco Science,27(2):48-51.
19 Maag D.,Dalvit C.,Thevenet D.,K?hler A.,Wouters F.C.,Vass?o D.G.,et al.(2014).3-β-d-Glucopyranosyl-6-methoxy-2-benzoxazolinone(MBOA-N-Glc)is an insect detoxification product of maize 1,4-benzoxazin-3-ones.Phytochemistry.102,97-105.doi:10.1016/j.phytochem.2014.03.018
20 Morris PF,Ward EWB(1992)Chemoattraction of zoospores of the soybean pathogen,Phytophthora sojae,by isoflavones.Physiological and Molecular Plant Pathology 1992,40:17-22.
21 Muehlstein LK,Amon JP,Leffler DL(1988)Chemotaxis in the marine fungus Rhizophydium littoreum.Appl Environm Microbiol 54,1668-1672.
22 Niemeyer H.M.(2009).Hydroxamic acids derived from 2-hydroxy-2 H-1,4-benzoxazin-3(4 H)-one:key defense chemicals of cereals.J.Agr.Food Chem.57,1677-1696.doi:10.1021/jf8034034
23 Osbourn AE.Preformed antimicrobial compounds and plant defense against fungal attack.Plant Cell,1996,8:1821-1831.
24 Park WJ,Hochholdinger F,Gierl A(2004)Release of the benzoxazinoids defense molecules during lateral-and crown root emergence in Zea mays.Journal of Plant Physiology,161,981-985.
25 Sun J,Li X,Wu Z,Sun H(2011).Progress on tobacco black shank disease.Hubei Agricultural Science,50(16):3253-3256.
26 Sun Y,Zhou T,Wang Y,Chen J,He X,et al.(2006)Effect of intercropping on disease management and yield of chilli pepper and maize.Acta Horticulturae Sinica 33,995-1000.
27 Tang CS,Young CC(1982)Collection and identification of allelopathic compounds from the undisturbed root system of bigalta limpograss(Hemarthria altissima).Plant Physiology 69,155-160.
28 Van West P,Morris BM,Reid B,Appiah A,Osborne MC,et al.(2002)Oomycete plant pathogens use electric fields to target roots.Molecular Plant-Microbe Interactions,2002,15:790-798.
29 Woodward MD,Corcuera LJ,Helgeson JP,Upper CD(1978)Decomposition of2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in aqueous solutions.Plant Physiology 61,796-802.
30 Yang M,Zhang Y,Qi L,Mei X,Liao J,et al.(2014)Plant-Plant-Microbe Mechanisms Involved in Soil-Borne Disease Suppression on a Maize and Pepper Intercropping System.PLo S ONE 9(12):e115052.doi:10.1371/journal.pone.0115052
31 Zhang J,Boone L,Kocz R,Zhang C,Binns AN,et al.(2000)At the maize/Agrobacterium interface:natural factors limiting host transformation.Chemistry&Biology 7,611-621.
32 Zhu SS,Liu XL,Liu PF,Li JQ,Wang HM,et al.(2007)Flumorph is a novel fungicide that disrupts microfilament organization in Phytophthora melonis.Phytopathology 97,643-649.
2 Bais HP,Park SW,Weir TL,Callaway RM,Vivanco JM.2004.How plants communicate using the underground information superhighway.Trends Plant Sci.9:26-32
3 Bais HP,Weir TL,Perry LG,Gilroy S,Vivanco JM.The role of root exudates in rhizosphere interactions with plants and other.Annu.Rev.Plant Biol.2006.57:233-266
4 Bjostard LB,Hibbard BE(1992)6-Methoxy-2-benzoxazotinone:A semiochemical for host location by Western corn root worm 1arvae.Journal of Chemical Ecology 18,931-944.
5 Bouws H,Finckh MR(2008)Effects of strip intercropping of potatoes with non-hosts on late blight severity and tuber yield in organic production.Plant Pathology 57,916-927.
6 Cameron JN,Carlile MJ(1978)Fatty acids,aldehydes and alcohols as attractants for zoospores of Phytophthora palmivora.Nature 271,448-449.
7 Carlile MJ(1983)Motility,taxis and tropism in Phytophthora.In Phytophthora:Its Biology,Taxonomy,Ecology and Pathology,Ed.by D.C.Erwin,S.Bartnicki-Garcia&P H Tsao,pp.95-107,American Phytopathological Society,St.Paul,Minnesota.
8 Cohen Y,Baider A,Cohen BH(1995)Dimethomorph activity against Oomycete fungal plant pathogens,Phytopathology,85,1500-1506.
9 Deacon JW(1988)Behavioural responses of fungal zoospores.Microbiological Sciences 5,249-252.
10 Erb M.,K?llner T.G.,Degenhardt J.,Zwahlen C.,Hibbard B.E.,and Turlings T.C.(2011).The role of abscisic acid and water stress in root herbivore-induced leaf resistance.New Phytol.189,308-320.doi:10.1111/j.1469-8137.2010.03450.x
11 Erwin DC,Ribeiro OK(1996)Phytophthora capsici.In:Erwin D.C.,Ribeiro O.K.(ed),Phytophthora Diseases Worldwide,pp.262-268.APS Press,St.Paul,MN,USA.
12 Erwin DC,Ribeiro OK(1996)Phytophthora capsici.In:Erwin D.C.,Ribeiro O.K.(ed),Phytophthora Diseases Worldwide,pp.262-268.APS Press,St.Paul,MN,USA.
13 Etzerodt T.,Nielsen S.T.,Mortensen A.G.,Christophersen C.,and Fomsgaard I.S.(2006).Elucidating the transformation pattern of the cereal allelochemical 6-methoxy-2-benzoxazolinone(MBOA)and the trideuteriomethoxy analogue[D3]-MBOAin soil.J.Agr.Food Chem.54,1075-1085.doi:10.1021/jf0509052
14 Fan KW,Vrijmoed LL,Jones EBG(2002)Zoospore chemotaxis of Mangrove thraustochytrids from Hong Kong.Mycologia 94,569-578.
15 Friebe A.,Vilich V.,Hennig L.,Kluge M.,and Sicker D.(1998).Detoxification of benzoxazolinone allelochemicals from wheat by Gaeumannomyces graminis var.tritici,G.graminis var.graminis,G.graminis var.avenae,and Fusarium culmorum.Appli.Environ.Microbiol.64,2386-2391.
16 He X,Zhu S,Wang H,Xie Y,Sun Y,et al.(2010)Crop diversity for disease ecological control in potato and maize.Journal of Resources and Ecology 1,45-50.
17 Kumar P,Gagliardo RW,Chilton WS(1993)Soil transformation of wheat and corn metabolites MBOA and DIMBOA into aminophenoxazinones.Journal of Chemical Ecology19,2453-2461.
18 Li T,Wang B,Wang S.1996.The current situations,problems and measures of tobacco rotation in Yunnan.Chinese Tobacco Science,27(2):48-51.
19 Maag D.,Dalvit C.,Thevenet D.,K?hler A.,Wouters F.C.,Vass?o D.G.,et al.(2014).3-β-d-Glucopyranosyl-6-methoxy-2-benzoxazolinone(MBOA-N-Glc)is an insect detoxification product of maize 1,4-benzoxazin-3-ones.Phytochemistry.102,97-105.doi:10.1016/j.phytochem.2014.03.018
20 Morris PF,Ward EWB(1992)Chemoattraction of zoospores of the soybean pathogen,Phytophthora sojae,by isoflavones.Physiological and Molecular Plant Pathology 1992,40:17-22.
21 Muehlstein LK,Amon JP,Leffler DL(1988)Chemotaxis in the marine fungus Rhizophydium littoreum.Appl Environm Microbiol 54,1668-1672.
22 Niemeyer H.M.(2009).Hydroxamic acids derived from 2-hydroxy-2 H-1,4-benzoxazin-3(4 H)-one:key defense chemicals of cereals.J.Agr.Food Chem.57,1677-1696.doi:10.1021/jf8034034
23 Osbourn AE.Preformed antimicrobial compounds and plant defense against fungal attack.Plant Cell,1996,8:1821-1831.
24 Park WJ,Hochholdinger F,Gierl A(2004)Release of the benzoxazinoids defense molecules during lateral-and crown root emergence in Zea mays.Journal of Plant Physiology,161,981-985.
25 Sun J,Li X,Wu Z,Sun H(2011).Progress on tobacco black shank disease.Hubei Agricultural Science,50(16):3253-3256.
26 Sun Y,Zhou T,Wang Y,Chen J,He X,et al.(2006)Effect of intercropping on disease management and yield of chilli pepper and maize.Acta Horticulturae Sinica 33,995-1000.
27 Tang CS,Young CC(1982)Collection and identification of allelopathic compounds from the undisturbed root system of bigalta limpograss(Hemarthria altissima).Plant Physiology 69,155-160.
28 Van West P,Morris BM,Reid B,Appiah A,Osborne MC,et al.(2002)Oomycete plant pathogens use electric fields to target roots.Molecular Plant-Microbe Interactions,2002,15:790-798.
29 Woodward MD,Corcuera LJ,Helgeson JP,Upper CD(1978)Decomposition of2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one in aqueous solutions.Plant Physiology 61,796-802.
30 Yang M,Zhang Y,Qi L,Mei X,Liao J,et al.(2014)Plant-Plant-Microbe Mechanisms Involved in Soil-Borne Disease Suppression on a Maize and Pepper Intercropping System.PLo S ONE 9(12):e115052.doi:10.1371/journal.pone.0115052
31 Zhang J,Boone L,Kocz R,Zhang C,Binns AN,et al.(2000)At the maize/Agrobacterium interface:natural factors limiting host transformation.Chemistry&Biology 7,611-621.
32 Zhu SS,Liu XL,Liu PF,Li JQ,Wang HM,et al.(2007)Flumorph is a novel fungicide that disrupts microfilament organization in Phytophthora melonis.Phytopathology 97,643-649.