Characterization and expression profiles of MaACS and MaACO genes from mulberry(Morus alba L.)
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- 英文论文题名：Characterization and expression profiles of MaACS and MaACO genes from mulberry(Morus alba L.)
- 论文作者：Chang-ying LIU ; Rui-hua Lü ; Jun LI ; Ai-chun ZHAO ; Xi-ling WANG ; Umuhoza DIANE ; Xiao-hong WANG ; Chuan-hong WANG ; Ya-sheng YU ; Shu-mei HAN ; Cheng LU ; Mao-de YU
- 英文论文作者：Chang-ying LIU ; Rui-hua Lü ; Jun LI ; Ai-chun ZHAO ; Xi-ling WANG ; Umuhoza DIANE ; Xiao-hong WANG ; Chuan-hong WANG ; Ya-sheng YU ; Shu-mei HAN ; Cheng LU ; Mao-de YU ; College of Biotechnology ; Southwest University
- 年：2016
- 作者机构：College of Biotechnology, Southwest University;
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S888.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：469k
- 原文格式：D
- 会议级别：全国
摘要
1-Aminocyclopropane-1-carboxylic acid synthase(ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase(ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type Ⅰ, MaACS3 and MaACS4 are type Ⅱ, and MaACS5 is type Ⅲ, with different C-terminal sequences. Quantitative reverse transcriptase polymerase chain reaction(qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demonstrated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid(ABA) and ethephon.
1-Aminocyclopropane-1-carboxylic acid synthase(ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase(ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type Ⅰ, MaACS3 and MaACS4 are type Ⅱ, and MaACS5 is type Ⅲ, with different C-terminal sequences. Quantitative reverse transcriptase polymerase chain reaction(qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demonstrated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid(ABA) and ethephon.
1-Aminocyclopropane-1-carboxylic acid synthase(ACS) and 1-aminocyclopropane-1-carboxylic acid oxidase(ACO) are encoded by multigene families and are involved in fruit ripening by catalyzing the production of ethylene throughout the development of fruit. However, there are no reports on ACS or ACO genes in mulberry, partly because of the limited molecular research background. In this study, we have obtained five ACS gene sequences and two ACO gene sequences from Morus Genome Database. Sequence alignment and phylogenetic analysis of MaACO1 and MaACO2 showed that their amino acids are conserved compared with ACO proteins from other species. MaACS1 and MaACS2 are type Ⅰ, MaACS3 and MaACS4 are type Ⅱ, and MaACS5 is type Ⅲ, with different C-terminal sequences. Quantitative reverse transcriptase polymerase chain reaction(qRT-PCR) expression analysis showed that the transcripts of MaACS genes were strongly expressed in fruit, and more weakly in other tissues. The expression of MaACO1 and MaACO2 showed different patterns in various mulberry tissues. MaACS and MaACO genes demonstrated two patterns throughout the development of mulberry fruit, and both of them were strongly up-regulated by abscisic acid(ABA) and ethephon.
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