白姜花miRNA167的克隆及靶基因的预测
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- 英文论文题名：The MiRNA167 Cloning and Prediction of Target Genes in Hedychium coronarium
- 论文作者：何磊 ; 范燕萍
- 英文论文作者：HE Lei ; FAN Yan-ping ; Cellege of Forestry and Landscape Architecture ; Flower Research Center ; South China Agricultural University
- 年：2016
- 作者机构：华南农业大学林学与风景园林学院华南农业大学花卉研究中心;
- 论文关键词：miRNA167 ; 靶基因 ; 白姜花 ; 花香
- 英文论文关键词：miRNA167 ; Target genes ; H.coronarium ; Flower fragrance
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.19
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：5
- 文件大小：477k
- 原文格式：O
- 会议级别：全国
摘要
MiRNA是小的RNA调节分子,长度一般在20个碱基左右,通过与目标mRNA结合而导致mRNA的解链或者是抑制其翻译。研究表明,拟南芥miRNA167参与调节花器官的发育,然而miRNA167在姜科植物中功能还未见报道。白姜花作为一种芳香植物,具有浓郁的花香,且花香物质释放与花的发育进程密切相关。本研究克隆了白姜花中miRNA167家族的miRNA167a和miRNA167k并对其进行了靶基因的预测及靶基因功能域的分析,为研究姜花miRNA167调控花香物质合成的分子机制奠定基础。
The microRNAs were a small regulation RNA molecules of 20 bp approximately.It can combine with target mRNA and cause chain mRNA solution or inhibit its translation.In Arabidopsis,miRNA167 was proved to regulate the development of flower organs.However,the function of miRNA167 has not been reported in Zingiberaceae plants.As a kind of fragrant plant,H.coronarium emit a large array of volatile scent,which closely related with the development of flower.In this study,miRNA167 a and miRNA167 k were cloned from H.coronarium.Target genes and target genes function domains were predicted.It provided the foundation basis for studying the molecular mechanism of miRNA167 regulating the volatile scent of H.coronarium.
The microRNAs were a small regulation RNA molecules of 20 bp approximately.It can combine with target mRNA and cause chain mRNA solution or inhibit its translation.In Arabidopsis,miRNA167 was proved to regulate the development of flower organs.However,the function of miRNA167 has not been reported in Zingiberaceae plants.As a kind of fragrant plant,H.coronarium emit a large array of volatile scent,which closely related with the development of flower.In this study,miRNA167 a and miRNA167 k were cloned from H.coronarium.Target genes and target genes function domains were predicted.It provided the foundation basis for studying the molecular mechanism of miRNA167 regulating the volatile scent of H.coronarium.
引文
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13.Voinnet O.2009.Origin,biogenesis,and activity of plant microRNAs[J].Cell,136:669-687.
14.Wu M.F,Tian Q and Reed J W.2006.Arabidopsis micro RNA167 controls patterns of ARF6 and ARF8 expression,and regulates both female and male reproduction[J].Development,133:4211-4218.
15.Yiguo Hong and Stephen Jacksoa 2015.Floral induction and flower formation—the role and potential applications of miRNAs[J].Plant Biotechnology Journal,13:282-292.
16.YueYC,Yu RC,Fan YP.2014.Characterization of two monoterpene synthases involved in floral scent formation in Hedychium coronarium[J].Plant,240(7):745-762.
17.Yue YC,Yu RC,Fan YP.2015.Transcriptome profiling provides new insights into the formation of floral scent in Hedychium coronarium[J].Bms Genomice,16:470,Doi:10.1186/s12864-015-1653-7.
18.Zeng SH,Liu YL,Pan LZ et al.2015.Identification and characterization of miRNAs in ripening fruit of Lycium barbarian L.using high-throughput sequencing[J].Frontiers in Plant Science,6:778,Doi:10.3389/fpls.2015.00778.
2.Fal Kateryna,Landrein Benoit,Hamant Olivier.2016.Interplay between miRNA regulation and mechanical stress for CUC gene expression at the shoot apical meristem[J].Plant signaling&behavior,11(3):el 127497,Doi:10.1080/15592324.2015.1127497.
3.Gindullis Frank,Rose Annkatrin,Patel Shalaka,Meier Iris.2002.Four signature motifs define the first class of structurally related large coiled-coil proteins in plants[J].Bmc Genomics,3(9):1-11.
4.Julien Curaba,Mohan B Singh and Prem L Bhalla.2014.miRNAs in the crosstalk between phytohormone signalling Pathways[J].Journal of Experimental Botany,65(6):1425-1438.
5.LiS,Liu L,ZhuangX,et al.2013.MicroRNAs inhibit the|translation of target mRNAs on the endoplasmic reticulum in Arabidopsis[J].Cell,153:562-574.
6.Muhlemann J K,Maeda H,Chang C,et al.2012.Developmental changes in the metabolic network of snapdragon flowersPlosOne,7:e403817,Doi:10.1371/journal.pone.0040381.
7.Nagpal P,Ellis CM,Weber H,Ploense SE,Barkawi LS,et al.2005.Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation[J].Development,132:4107-4118.
8.Reeves PH,Ellis CM,Ploense SE,Wu MF,Yadav V,et al.2012.A regulatory network for coordinated flower maturation[J].Plos Genet,8:el002506,Doi:10.1371/journal.pgen.1002-506.
9.Rubio-Somoza I and Weigel D.2011.MicroRNA networks and developmental plasticity in plants[J].Trends Plant Science,16:258-264.
10.Ru P,Xu L,Ma H and Huang H.2006.Plant fertility defects induced by the enhanced expression of microRNA167[J].Cell Research,16:457-465.
11.Sun RR,GuoTL,Cobb J,Wang QL,Zhang BH.2015.Role of microRNAs During Flower and Storage Root Development in sweet Potato[J].Plant Molecular Biology Reporter,33:1731-1739.
12.TabataR,Ikezaki M,Fujibe T,AidaM,Tian CE,et al.2010.Arabidopsis AUXIN RESPONSE FACTOR6 and 8regulate jasmonic acid biosynthesis and floral organ development via repression of class 1 KNOX genes[J].Plant Cell Physiology,51:164-175.
13.Voinnet O.2009.Origin,biogenesis,and activity of plant microRNAs[J].Cell,136:669-687.
14.Wu M.F,Tian Q and Reed J W.2006.Arabidopsis micro RNA167 controls patterns of ARF6 and ARF8 expression,and regulates both female and male reproduction[J].Development,133:4211-4218.
15.Yiguo Hong and Stephen Jacksoa 2015.Floral induction and flower formation—the role and potential applications of miRNAs[J].Plant Biotechnology Journal,13:282-292.
16.YueYC,Yu RC,Fan YP.2014.Characterization of two monoterpene synthases involved in floral scent formation in Hedychium coronarium[J].Plant,240(7):745-762.
17.Yue YC,Yu RC,Fan YP.2015.Transcriptome profiling provides new insights into the formation of floral scent in Hedychium coronarium[J].Bms Genomice,16:470,Doi:10.1186/s12864-015-1653-7.
18.Zeng SH,Liu YL,Pan LZ et al.2015.Identification and characterization of miRNAs in ripening fruit of Lycium barbarian L.using high-throughput sequencing[J].Frontiers in Plant Science,6:778,Doi:10.3389/fpls.2015.00778.