IVIG Therapy Ameliorates Kawasaki Disease by Regulating NK and CD8-related cytotoxic activity and Tregs expression
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- 英文论文题名：IVIG Therapy Ameliorates Kawasaki Disease by Regulating NK and CD8-related cytotoxic activity and Tregs expression
- 论文作者：Huang Juan ; Tu Wenwei
- 英文论文作者：Huang Juan ; Tu Wenwei ; Department of Hematology ; Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital ; Joint Research Center of West China Second University Hospital of Sichuan University and Department of Paediatrics and Adolescent Medicine of University of Hong Kong ; Sichuan University
- 年：2016
- 作者机构：Department of Hematology,Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital;Joint Research Center of West China Second University Hospital of Sichuan University and Department of Paediatrics and Adolescent Medicine of University of Hong Kong,Sichuan University;
- 英文论文关键词：Kawasaki Disease ; innate and adaptive immunity ; cytotoxicity ; Tregs
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R725.4
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：1320k
- 原文格式：D
- 会议级别：全国
摘要
Purpose: Kawasaki disease(KD) is a pediatric self-limited vasculitis characterized by immune-mediated destruction of the arterial wall and myocardium. Treatment with IVIG plus Aspirin is the effective cure for this illness. However its etiology and the mechanism of IVIG treatment are poorly understood. Here, thirty-eight KD patients were enrolled to further understanding the immune response in KD patients with IVIG treatment.Methods: PBMC from KD patients with or without IVIG treatment were collected at indicated times during the acute phase:(i) before treated with IVIG,(ii) days 2-3 after treated with IVIG and(iii) days 5-7 after treated with IVIG. And then we examined the cytotoxic activity of PBMC against K562, the frequency of immune cells, and their underlying mechanisms by flow cytometry(gallios). Results: We found that IVIG treatment could activate and expanded NK cells and CD8~+ T cells, that efficiently killed K562 cells. Perforin-granzyme B pathway were involved in their cytotoxicity.Treg expansion,T GF-beta release were present at the acute phase of KD.Conclusions: In conclusion, Our findings may potentially provide an immulogic menchanism to understand for IVIG treatment of KD patients, that may influence the strategies to treat and monitor patients with Kawasaki disease.
Purpose: Kawasaki disease(KD) is a pediatric self-limited vasculitis characterized by immune-mediated destruction of the arterial wall and myocardium. Treatment with IVIG plus Aspirin is the effective cure for this illness. However its etiology and the mechanism of IVIG treatment are poorly understood. Here, thirty-eight KD patients were enrolled to further understanding the immune response in KD patients with IVIG treatment.Methods: PBMC from KD patients with or without IVIG treatment were collected at indicated times during the acute phase :(i) before treated with IVIG,(ii) days 2-3 after treated with IVIG and(iii) days 5-7 after treated with IVIG. And then we examined the cytotoxic activity of PBMC against K562, the frequency of immune cells, and their underlying mechanisms by flow cytometry(gallios). Results: We found that IVIG treatment could activate and expanded NK cells and CD8+ T cells, that efficiently killed K562 cells. Perforin-granzyme B pathway were involved in their cytotoxicity.Treg expansion,T GF-beta release were present at the acute phase of KD.Conclusions: In conclusion, Our findings may potentially provide an immulogic menchanism to understand for IVIG treatment of KD patients, that may influence the strategies to treat and monitor patients with Kawasaki disease.
Purpose: Kawasaki disease(KD) is a pediatric self-limited vasculitis characterized by immune-mediated destruction of the arterial wall and myocardium. Treatment with IVIG plus Aspirin is the effective cure for this illness. However its etiology and the mechanism of IVIG treatment are poorly understood. Here, thirty-eight KD patients were enrolled to further understanding the immune response in KD patients with IVIG treatment.Methods: PBMC from KD patients with or without IVIG treatment were collected at indicated times during the acute phase :(i) before treated with IVIG,(ii) days 2-3 after treated with IVIG and(iii) days 5-7 after treated with IVIG. And then we examined the cytotoxic activity of PBMC against K562, the frequency of immune cells, and their underlying mechanisms by flow cytometry(gallios). Results: We found that IVIG treatment could activate and expanded NK cells and CD8+ T cells, that efficiently killed K562 cells. Perforin-granzyme B pathway were involved in their cytotoxicity.Treg expansion,T GF-beta release were present at the acute phase of KD.Conclusions: In conclusion, Our findings may potentially provide an immulogic menchanism to understand for IVIG treatment of KD patients, that may influence the strategies to treat and monitor patients with Kawasaki disease.
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