Engineering nitrogen use efficiency in wheat
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- 英文论文题名：Engineering nitrogen use efficiency in wheat
- 论文作者：Yiping Tong ; Hui Li ; Mengyun Hu ; Xueqiang Zhao ; Xue He ; Baoyuan Qu ; Wenjing Li
- 英文论文作者：Yiping Tong ; Hui Li ; Mengyun Hu ; Xueqiang Zhao ; Xue He ; Baoyuan Qu ; Wenjing Li ; State Key Laboratory of Plant Cell and Chromosome Engineering ; Institute of Genetics and Developmental biology ; Chinese Academy of Sciences ; The Institute for Cereal and Oil Crops ; Hebei Academy of Agriculture and Forestry Sciences
- 年：2016
- 作者机构：State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental biology,Chinese Academy of Sciences;The Institute for Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences;
- 英文论文关键词：Triticum aestivum L ; NAC2 ; glutamine synthetase ; nitrate uptake ; lateral root ; grain yield ; grain nitrogen concentration ; nitrogen use efficiency
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S512.1
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：895k
- 原文格式：D
- 会议级别：全国
摘要
Developing wheat varieties with improved ability to use nitrogen(N) efficiently is very desirable,and may offer a sustainable solution to improve crop yields with less fertilizer application. Roots are the main site for nutrient uptake; their size and distribution in soil profiles,and uptake activity largely determine nutrient uptake efficiency. However,at seedling stage,low temperature inhibits root development of winter wheat and nutrient bioavailability in soils; and during grain filling root senescence is not able to meet the increased N demand of high yield potential of modern wheat varieties. Here,we developed transgenic wheat lines with improved NUE by increasing roots′ ability in acquiring N at seedling stage and N uptake after flowering. We showed that a nitrate-inducible NAC transcription factor TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase(GS). Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate,and hence increase root ability to acquire nitrogen at seedling stage. Further,we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts,and allocated more nitrogen in grains in a field experiment. We further analyzed the haplotypes of the plastic GS isoform(GS2) genes in wheat. The transgenic wheat lines of the favorable TaGS2 haplotypes TaGS2-A1bpro::TaGS2-A1 b had higher N uptake after flowering and grain yield than did the wild type under both low and high N conditions in field experiments.
Developing wheat varieties with improved ability to use nitrogen(N) efficiently is very desirable,and may offer a sustainable solution to improve crop yields with less fertilizer application. Roots are the main site for nutrient uptake; their size and distribution in soil profiles,and uptake activity largely determine nutrient uptake efficiency. However,at seedling stage,low temperature inhibits root development of winter wheat and nutrient bioavailability in soils; and during grain filling root senescence is not able to meet the increased N demand of high yield potential of modern wheat varieties. Here,we developed transgenic wheat lines with improved NUE by increasing roots′ ability in acquiring N at seedling stage and N uptake after flowering. We showed that a nitrate-inducible NAC transcription factor TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase(GS). Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate,and hence increase root ability to acquire nitrogen at seedling stage. Further,we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts,and allocated more nitrogen in grains in a field experiment. We further analyzed the haplotypes of the plastic GS isoform(GS2) genes in wheat. The transgenic wheat lines of the favorable TaGS2 haplotypes TaGS2-A1bpro::TaGS2-A1 b had higher N uptake after flowering and grain yield than did the wild type under both low and high N conditions in field experiments.
Developing wheat varieties with improved ability to use nitrogen(N) efficiently is very desirable,and may offer a sustainable solution to improve crop yields with less fertilizer application. Roots are the main site for nutrient uptake; their size and distribution in soil profiles,and uptake activity largely determine nutrient uptake efficiency. However,at seedling stage,low temperature inhibits root development of winter wheat and nutrient bioavailability in soils; and during grain filling root senescence is not able to meet the increased N demand of high yield potential of modern wheat varieties. Here,we developed transgenic wheat lines with improved NUE by increasing roots′ ability in acquiring N at seedling stage and N uptake after flowering. We showed that a nitrate-inducible NAC transcription factor TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase(GS). Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate,and hence increase root ability to acquire nitrogen at seedling stage. Further,we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts,and allocated more nitrogen in grains in a field experiment. We further analyzed the haplotypes of the plastic GS isoform(GS2) genes in wheat. The transgenic wheat lines of the favorable TaGS2 haplotypes TaGS2-A1bpro::TaGS2-A1 b had higher N uptake after flowering and grain yield than did the wild type under both low and high N conditions in field experiments.
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