基于VOCs分子标志物快速质谱鉴定大肠埃希菌
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- 论文作者：杭亚平 ; 梁钜超 ; 王小中 ; 刘衍伶 ; 胡晓彦 ; 章白苓 ; 胡龙华
- 年：2016
- 作者机构：南昌大学第二附属医院检验科;
- 论文关键词：E.coli ; 吲哚 ; SDAPCI-MS ; VOCs ; 鉴定
- 英文论文关键词：Escherichia coli ; indole ; Surface analytical atmospheric pressure chemical ionization mass spectrometry ; volatile organic compounds ; identification
- 会议召开时间：2016-04-26
- 会议录名称：中国化学会第十一届全国生物医药色谱及相关技术学术交流会（大会特邀报告及墙报）论文摘要集
- 语种：中文
- 分类号：R446.5
- 学会代码：ZGHY
- 会议名称：中国化学会第十一届全国生物医药色谱及相关技术学术交流会
- 会议地点：中国江西井冈山
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：2
- 文件大小：249k
- 原文格式：D
- 会议级别：全国
摘要
目的探讨表面解析常压化学电离质谱(SDAPCI-MS)检测大肠埃希菌(E.coli)的挥发性有机代谢产物(VOCs)对快速鉴定E.coli的临床价值。方法 BACT/ALERT 3D全自动血培养仪进行增菌培养。VITEK2-compact全自动微生物分析仪进行菌种鉴定。SDAPCI-MS检测菌落上层气体和血培养瓶上层气体VOCs。结果 m/z 116特异地仅存于E.coli中,且其信号强度与培养时间和细菌初始浓度成正比。M/z 116经二级质谱确定为吲哚。结论 VOCs的快速SDAPCI-MS检测,操作简便、重复性高、鉴定结果可靠,生物标志物吲哚尤为适用于临床E.coli的鉴定。
Objective The investigation on volatile organic compounds(VOCs) of common pathogens by Surface analytical atmospheric pressure chemical ionization mass spectrometry(SDAPCI-MS) analysis for the clinical value of E.coli identification. Methods BACT/ALERT 3D automated blood cultures instrument enriched the bacteria. Identified by VITEK2-compact automated microbial analyze. VOCs of solid cultures and blood culture were detected by SDAPCI-MS. Results m/z 116 specifically only found in E.coli, and the signal strength was proportional to the incubation time and the initial concentration of bacteria. M/z 116 was determined by tandem mass spectrometry as indole. Conclusion Fast SDAPCI-MS detection of VOCs, is an easy operation, with high repeatability, reliable identification results, biomarkers indole is particularly suitable for the identification of clinical E.coli.
Objective The investigation on volatile organic compounds(VOCs) of common pathogens by Surface analytical atmospheric pressure chemical ionization mass spectrometry(SDAPCI-MS) analysis for the clinical value of E.coli identification. Methods BACT/ALERT 3D automated blood cultures instrument enriched the bacteria. Identified by VITEK2-compact automated microbial analyze. VOCs of solid cultures and blood culture were detected by SDAPCI-MS. Results m/z 116 specifically only found in E.coli, and the signal strength was proportional to the incubation time and the initial concentration of bacteria. M/z 116 was determined by tandem mass spectrometry as indole. Conclusion Fast SDAPCI-MS detection of VOCs, is an easy operation, with high repeatability, reliable identification results, biomarkers indole is particularly suitable for the identification of clinical E.coli.
引文
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11 .J.Zhu,H.D.Bean,Y.M.Kuo,et al.,Fast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry,J Clin Microbiol,2010,48:4426-4431
2 .E.Kuenzli,V.K.Jaeger,R.Frei,et al.,High colonization rates of extended-spectrum beta-lactamase(esbl)-producing escherichia coli in swiss travellers to south asia-a prospective observational multicentre cohort study looking at epidemiology,microbiology and risk factors,BMC Infect Dis,2014,14:528
3 .C.C.Lai,Y.H.Chen,S.H.Lin,et al.,Changing aetiology of healthcare-associated bloodstream infections at three medical centres in taiwan,2000-2011,Epidemiol Infect,2014,142:2180-2185
4 .S.Q.van Veen,E.C.Claas,E.J.Kuijper,High-throughput identification of bacteria and yeast by matrix-assisted laser desorption ionization-time of flight mass spectrometry in conventional medical microbiology laboratories,J Clin Microbiol,2010,48:900-907
5 .M.C.Lemfack,J.Nickel,M.Dunkel,et al.,Mvoc:A database of microbial volatiles,Nucleic Acids Res,2014,42:D744-748
6 .B.Hu,X.Zhang,M.Li,et al.,Coupling corona discharge for ambient extractive ionization mass spectrometry,Analyst,2011,136:4977-4985
7 .汪红,宁长秀,钟桥石,et al.,2012年临床分离菌分布及耐药性分析,中国感染与化疗杂志,2014,14:63-68
8 .黄敏,呼吸内科下呼吸道感染病原菌分布及耐药性分析,中国伤残医学,2015,23:139-140
9 .陈娜,苏建荣,血培养阳性混合菌感染的病原菌分布及耐药性分析,临床和实验医学杂志,2015,14:512-515
10 .M.Junger,W.Vautz,M.Kuhns,et al.,Ion mobility spectrometry for microbial volatile organic compounds:A new identification tool for human pathogenic bacteria,Appl Microbiol Biotechnol,2012,93:2603-2614
11 .J.Zhu,H.D.Bean,Y.M.Kuo,et al.,Fast detection of volatile organic compounds from bacterial cultures by secondary electrospray ionization-mass spectrometry,J Clin Microbiol,2010,48:4426-4431