Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production
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- 英文论文题名：Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production
- 论文作者：Yang Wang ; Yanping Wu ; Yibing Wang ; Aikun Fu ; Li Gong ; Weifen Li ; Yali Li
- 英文论文作者：Yang Wang ; Yanping Wu ; Yibing Wang ; Aikun Fu ; Li Gong ; Weifen Li ; Yali Li ; Key Laboratory of Molecular Animal Nutrition of the Ministry of Education ; Institute of Feed Science ; College of Animal Sciences ; Zhejiang University
- 年：2016
- 作者机构：Key Laboratory of Molecular Animal Nutrition of the Ministry of Education, Institute of Feed Science,College of Animal Sciences, Zhejiang University;
- 英文论文关键词：Bacillus ; IPEC-1 ; Oxidative stress ; Nrf2 ; ROS ; NOX
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：英文
- 分类号：S816
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：76k
- 原文格式：O
- 会议级别：全国
摘要
Oxidative stress(OS) plays a major role in the gastrointestinal disorders.Although probiotics were reported to repress OS,few researches compared the antioxidant ability of different Bacillus strains and decipher the mechanisms.To select a Bacillus strain with higher antioxidant capacity,we used H_2O_2 to induce intestinal porcine epithelial cell 1(IPEC-1) OS model.The most suitable H_2O_2 concentration and incubation time were determined by the half lethal dose and Methyl Thiazolyl Tetrazolium.Correlation analysis was performed to choose a sensitive indicator for OS.As for the comparison of Bacillus,cells were divided into:control,Bacillus treatment,H_2O_2 treatment and Bacillus pre-protection+H_2O_2 treatment.Bacillus were co-cultured with IPEC-1 for 3h in Bacillus and Bacillus pre-protection+H_2O_2 treatments.Then,based on OS model,300 μmol/L H_2O_2 were added into medium of H_2O_2 and Bacillus pre-protection+H_2O_2 treatments for another 12 h.Antioxidant and apoptosis gene expressions were detected to screen the target strain.Nuclear factor erythroid-derived 2 related factor 2(Nrf2)/Kelch-like ECH-associated protein 1(Keap1) pathway,reactive oxygen species(ROS) production,mitochondrial membrane potential(△ψm),apoptosis and necrosis were analyzed.Results revealed that heme oxygenase-1(HO-1) gene expression had a positive correlation with H_2O_2 induction.Moreover,Bacillus amyloliquefaciens SC06(SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation.△ψm was elevated while ROS generation was reduced with SC06 pre-protection,resulting in decreased apoptosis and necrosis.Altogether,HO-1 expression could be regarded as an OS indicator.The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
Oxidative stress(OS) plays a major role in the gastrointestinal disorders.Although probiotics were reported to repress OS,few researches compared the antioxidant ability of different Bacillus strains and decipher the mechanisms.To select a Bacillus strain with higher antioxidant capacity,we used H_2O_2 to induce intestinal porcine epithelial cell 1(IPEC-1) OS model.The most suitable H_2O_2 concentration and incubation time were determined by the half lethal dose and Methyl Thiazolyl Tetrazolium.Correlation analysis was performed to choose a sensitive indicator for OS.As for the comparison of Bacillus,cells were divided into:control,Bacillus treatment,H_2O_2 treatment and Bacillus pre-protection+H_2O_2 treatment.Bacillus were co-cultured with IPEC-1 for 3h in Bacillus and Bacillus pre-protection+H_2O_2 treatments.Then,based on OS model,300 μmol/L H_2O_2 were added into medium of H_2O_2 and Bacillus pre-protection+H_2O_2 treatments for another 12 h.Antioxidant and apoptosis gene expressions were detected to screen the target strain.Nuclear factor erythroid-derived 2 related factor 2(Nrf2)/Kelch-like ECH-associated protein 1(Keap1) pathway,reactive oxygen species(ROS) production,mitochondrial membrane potential(△ψm),apoptosis and necrosis were analyzed.Results revealed that heme oxygenase-1(HO-1) gene expression had a positive correlation with H_2O_2 induction.Moreover,Bacillus amyloliquefaciens SC06(SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation.△ψm was elevated while ROS generation was reduced with SC06 pre-protection,resulting in decreased apoptosis and necrosis.Altogether,HO-1 expression could be regarded as an OS indicator.The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
Oxidative stress(OS) plays a major role in the gastrointestinal disorders.Although probiotics were reported to repress OS,few researches compared the antioxidant ability of different Bacillus strains and decipher the mechanisms.To select a Bacillus strain with higher antioxidant capacity,we used H_2O_2 to induce intestinal porcine epithelial cell 1(IPEC-1) OS model.The most suitable H_2O_2 concentration and incubation time were determined by the half lethal dose and Methyl Thiazolyl Tetrazolium.Correlation analysis was performed to choose a sensitive indicator for OS.As for the comparison of Bacillus,cells were divided into:control,Bacillus treatment,H_2O_2 treatment and Bacillus pre-protection+H_2O_2 treatment.Bacillus were co-cultured with IPEC-1 for 3h in Bacillus and Bacillus pre-protection+H_2O_2 treatments.Then,based on OS model,300 μmol/L H_2O_2 were added into medium of H_2O_2 and Bacillus pre-protection+H_2O_2 treatments for another 12 h.Antioxidant and apoptosis gene expressions were detected to screen the target strain.Nuclear factor erythroid-derived 2 related factor 2(Nrf2)/Kelch-like ECH-associated protein 1(Keap1) pathway,reactive oxygen species(ROS) production,mitochondrial membrane potential(△ψm),apoptosis and necrosis were analyzed.Results revealed that heme oxygenase-1(HO-1) gene expression had a positive correlation with H_2O_2 induction.Moreover,Bacillus amyloliquefaciens SC06(SC06)-meditated IPEC-1 showed the best antioxidant capacity though modulating Nrf2 phosphorylation.△ψm was elevated while ROS generation was reduced with SC06 pre-protection,resulting in decreased apoptosis and necrosis.Altogether,HO-1 expression could be regarded as an OS indicator.The regulation of Nrf2/Keap1 pathway and ROS production by SC06 are involved in alleviating OS of IPEC-1.
引文
Amaretti A,di Nunzio M,Pompei A,Raimondi S,Rossi M,Bordoni A(2013)Antioxidant properties of potentially probiotic bacteria:in vitro and in vivo activities.Appl Microbiol Biotechnol 97:809-817