Parasitic antigens alter macrophage polarization during Schistosoma japonicum infection in mice
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- 英文论文题名：Parasitic antigens alter macrophage polarization during Schistosoma japonicum infection in mice
- 论文作者：Jifeng Zhu ; Zhipeng Xu ; Xiaojun Chen ; Sha Zhou ; Weiwei Zhang ; Ying Chi ; Wei Li ; Xian Song ; Feng Liu ; Chuan Su
- 英文论文作者：Jifeng Zhu ; Zhipeng Xu ; Xiaojun Chen ; Sha Zhou ; Weiwei Zhang ; Ying Chi ; Wei Li ; Xian Song ; Feng Liu ; Chuan Su ; Department of Pathogen Biology & Immunology ; Jiangsu Key Laboratory of Pathogen Biology ; Nanjing Medical University ; Key Laboratory of Enteric Pathogenic Microbiology ; Ministry of Health ; Institute of Pathogenic Microbiology ; Jiangsu Provincial Center for Disease Prevention and Control
- 年：2016
- 作者机构：Department of Pathogen Biology & Immunology, Jiangsu Key Laboratory of Pathogen Biology, Nanjing Medical University;Key Laboratory of Enteric Pathogenic Microbiology, Ministry of Health, Institute of Pathogenic Microbiology, Jiangsu Provincial Center for Disease Prevention and Control;
- 英文论文关键词：macrophage ; polarization ; Schistosoma japonicum
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R532.21
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：325k
- 原文格式：D
- 会议级别：全国
摘要
Objective To study the dynamics of macrophage polarization, as well as the possible factors that regulate macrophage polarization during schistosome infection. Methods We first analyzed M1 and M2-phenotypic markers of peritoneal macrophages from mice infected with Schistosoma japonicum(S. japonicum) at indicated time points using flow cytometry(FCM) analysis and real-time PCR. Then we treated peritoneal macrophages from normal mice with schistosome worm antigen(SWA) or schistosome soluble egg antigen(SEA) and determined M1 and M2-phenotypic markers, in order to identify macrophage polarization in responding to schistosomal antigens. Results In this study, we showed that macrophages were preferentially differentiated into M1 subtype during the acute stage of S. japonicum infection. However, the level of M1 macrophages decreased but M2 macrophages significantly increased during the chronic stage of infection. Furthermore, we showed that SWA favors the generation of M1 macrophages, whereas SEA preferentially promotes M2-polarized phenotype.
Objective To study the dynamics of macrophage polarization, as well as the possible factors that regulate macrophage polarization during schistosome infection. Methods We first analyzed M1 and M2-phenotypic markers of peritoneal macrophages from mice infected with Schistosoma japonicum(S. japonicum) at indicated time points using flow cytometry(FCM) analysis and real-time PCR. Then we treated peritoneal macrophages from normal mice with schistosome worm antigen(SWA) or schistosome soluble egg antigen(SEA) and determined M1 and M2-phenotypic markers, in order to identify macrophage polarization in responding to schistosomal antigens. Results In this study, we showed that macrophages were preferentially differentiated into M1 subtype during the acute stage of S. japonicum infection. However, the level of M1 macrophages decreased but M2 macrophages significantly increased during the chronic stage of infection. Furthermore, we showed that SWA favors the generation of M1 macrophages, whereas SEA preferentially promotes M2-polarized phenotype.
Objective To study the dynamics of macrophage polarization, as well as the possible factors that regulate macrophage polarization during schistosome infection. Methods We first analyzed M1 and M2-phenotypic markers of peritoneal macrophages from mice infected with Schistosoma japonicum(S. japonicum) at indicated time points using flow cytometry(FCM) analysis and real-time PCR. Then we treated peritoneal macrophages from normal mice with schistosome worm antigen(SWA) or schistosome soluble egg antigen(SEA) and determined M1 and M2-phenotypic markers, in order to identify macrophage polarization in responding to schistosomal antigens. Results In this study, we showed that macrophages were preferentially differentiated into M1 subtype during the acute stage of S. japonicum infection. However, the level of M1 macrophages decreased but M2 macrophages significantly increased during the chronic stage of infection. Furthermore, we showed that SWA favors the generation of M1 macrophages, whereas SEA preferentially promotes M2-polarized phenotype.
引文