Recombinant cystatin from Schistosoma japonicum therapeutically ameliorates CLP-induced Sepsis in mice
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- 英文论文题名：Recombinant cystatin from Schistosoma japonicum therapeutically ameliorates CLP-induced Sepsis in mice
- 论文作者：Xiaodi Yang ; Li-li Hui ; Wenxing He ; Yongkun Wan ; Xingzhi Chen ; Lansong Xu ; Renming Xue ; Rui Wang ; Hui Jiang ; Kun Peng ; Fang Qiang ; Shushu Wang
- 英文论文作者：Xiaodi Yang ; Li-li Hui ; Wenxing He ; Yongkun Wan ; Xingzhi Chen ; Lansong Xu ; Renming Xue ; Rui Wang ; Hui Jiang ; Kun Peng ; Fang Qiang ; Shushu Wang ; Department of Microbiology and Parasitology ; Anhui Key Laboratory of Infection and Immunity ; The Scientific Research Innovation Team Project of Anhui Colleges and Universities (2016-40) ; Bengbu Medical College ; Pediatrics Department of Affiliated Provincial Hospital ; Anhui Medical University
- 年：2016
- 作者机构：Department of Microbiology and Parasitology, Anhui Key Laboratory of Infection and Immunity, The Scientific Research Innovation Team Project of Anhui Colleges and Universities (2016-40), Bengbu Medical College;Pediatrics Department of Affiliated Provincial Hospital, Anhui Medical University;
- 英文论文关键词：sepsis ; Schistosoma japonicum ; recombinant cystatin ; therapeutic
- 会议召开时间：2016-08-08
- 会议录名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会论文摘要集
- 英文会议录名称：The Abstracts of the Tenth Symposium for Yong Scientists of China Society of Parasitology
- 语种：英文
- 分类号：R459.7
- 学会代码：JISC
- 会议名称：中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会
- 会议地点：中国四川成都
- 主办单位：中国动物学会寄生虫学专业青年委员会
- 学会名称：中国动物学会寄生虫学专业委员会
- 页数：1
- 文件大小：347k
- 原文格式：D
- 会议级别：全国
摘要
Objectives: Helminth or it's derived protein are potent inducers of T helper type 2(Th2) response and have a regulatory role, notably on allergic or autoimmune diseases. Now it's immunomodulation in host has also been shown to have therapeutic implications in sepsis which is characterised by the aberrant production of pro-inflammatory. Herein we have evaluated the therapeutic effect of recombinant cystatin from Schistosoma japonicum(r Sjcystatin) in a mice model of polymicrobial sepsis. Methods: The mice model with sepsis was established with cecal ligation and puncture(CLP). BALB/c mice were treated intraperitoneally(i.p.) with rB mC ys(10, 25 or 50 μg/dose) 0.5 h after the CLP operation, and persistent observation was performed for 72 h. Blood samples and tissues were collected at 12 h after CLP. Cytokines in serum were measured by ELISA. The pathological damage of liver and kidney tissue were stained with hematoxylin-eosin(HE), and the function of liver and kidney was examined 12 h after the CLP operation by fully automatic chemistry analyzer. Results: Treatment with r Sjcystatin in mice ameliorated the pathological damage degree and the increased serum levels of ALT, AST, BUN, and Cr caused by CLP-induced sepsis in liver and kidney of mice. While the level of TNF-α, IL-1β and IL-6 was down-regulated, the IL-10 and TGF-β expression were up-regulated in the serum of CLP-induced sepsis mice treated with r Sjcystatin. The amelioration effect in CLP-induced sepsis mice was in a dose-dependent manner.Conclusions: The results of this study indicate rS jcystatin can counterbalance exacerbated pro-inflammatory immune responses that occur during sepsis, improving survival. The anti-inflammatory effect of r Sjcystatin provides evidential support for this protein to be used as a promising therapeutic agent in sepsis.
Objectives: Helminth or it's derived protein are potent inducers of T helper type 2(Th2) response and have a regulatory role, notably on allergic or autoimmune diseases. Now it's immunomodulation in host has also been shown to have therapeutic implications in sepsis which is characterised by the aberrant production of pro-inflammatory. Herein we have evaluated the therapeutic effect of recombinant cystatin from Schistosoma japonicum(r Sjcystatin) in a mice model of polymicrobial sepsis. Methods: The mice model with sepsis was established with cecal ligation and puncture(CLP). BALB/c mice were treated intraperitoneally(i.p.) with rB mC ys(10, 25 or 50 μg/dose) 0.5 h after the CLP operation, and persistent observation was performed for 72 h. Blood samples and tissues were collected at 12 h after CLP. Cytokines in serum were measured by ELISA. The pathological damage of liver and kidney tissue were stained with hematoxylin-eosin(HE), and the function of liver and kidney was examined 12 h after the CLP operation by fully automatic chemistry analyzer. Results: Treatment with r Sjcystatin in mice ameliorated the pathological damage degree and the increased serum levels of ALT, AST, BUN, and Cr caused by CLP-induced sepsis in liver and kidney of mice. While the level of TNF-α, IL-1β and IL-6 was down-regulated, the IL-10 and TGF-β expression were up-regulated in the serum of CLP-induced sepsis mice treated with r Sjcystatin. The amelioration effect in CLP-induced sepsis mice was in a dose-dependent manner.Conclusions: The results of this study indicate rS jcystatin can counterbalance exacerbated pro-inflammatory immune responses that occur during sepsis, improving survival. The anti-inflammatory effect of r Sjcystatin provides evidential support for this protein to be used as a promising therapeutic agent in sepsis.
Objectives: Helminth or it's derived protein are potent inducers of T helper type 2(Th2) response and have a regulatory role, notably on allergic or autoimmune diseases. Now it's immunomodulation in host has also been shown to have therapeutic implications in sepsis which is characterised by the aberrant production of pro-inflammatory. Herein we have evaluated the therapeutic effect of recombinant cystatin from Schistosoma japonicum(r Sjcystatin) in a mice model of polymicrobial sepsis. Methods: The mice model with sepsis was established with cecal ligation and puncture(CLP). BALB/c mice were treated intraperitoneally(i.p.) with rB mC ys(10, 25 or 50 μg/dose) 0.5 h after the CLP operation, and persistent observation was performed for 72 h. Blood samples and tissues were collected at 12 h after CLP. Cytokines in serum were measured by ELISA. The pathological damage of liver and kidney tissue were stained with hematoxylin-eosin(HE), and the function of liver and kidney was examined 12 h after the CLP operation by fully automatic chemistry analyzer. Results: Treatment with r Sjcystatin in mice ameliorated the pathological damage degree and the increased serum levels of ALT, AST, BUN, and Cr caused by CLP-induced sepsis in liver and kidney of mice. While the level of TNF-α, IL-1β and IL-6 was down-regulated, the IL-10 and TGF-β expression were up-regulated in the serum of CLP-induced sepsis mice treated with r Sjcystatin. The amelioration effect in CLP-induced sepsis mice was in a dose-dependent manner.Conclusions: The results of this study indicate rS jcystatin can counterbalance exacerbated pro-inflammatory immune responses that occur during sepsis, improving survival. The anti-inflammatory effect of r Sjcystatin provides evidential support for this protein to be used as a promising therapeutic agent in sepsis.
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