摘要
总结了我国在中华绒螯蟹病毒病、细菌病、寄生虫病及非生物致病因子方面的病原学研究的主要成果,归纳中华绒螯蟹病毒性、细菌性、寄生虫性疾病的预防或治疗方法及免疫学研究。在病毒病研究方面,运用组织病理切片及超微组织病理切片、巢式PCR和回感实验等方法手段,确定的病毒病病原有RNA、ss RNA、WSSV、呼肠孤病毒等;在细菌病研究方面,运用细菌分离和培养、生物学性状、致病性及药物敏感性分析等技术方法,确定的细菌病致病菌有嗜水气单胞菌、弧菌、弗氏柠檬酸杆菌、点状产气单胞菌、维氏气单胞菌等;在寄生虫病研究方面,主要运用病理切片、显微镜观察等技术手段,确定的寄生虫病病原有微孢子虫、藤壶、利氏才女虫、固着类纤毛虫等;在非生物致病因子方面,运用生态学、组织学理论及方法,对重金属和营养及水质引发的疾病进行了研究;在预防和治疗方面,进行了免疫学研究,并从药物及免疫学角度运用药敏、毒力及免疫实验等技术方法进行预防或治疗研究。
Summarizes the main achievements of study on etiology of our country in the Eriocheir sinensis virus disease, bacterial disease, parasitic disease and non biological pathogenic factor.Summarized the Chinese mitten crab virus, bacterial, parasitic diseases prevention or treatment and immunological studies. In the study of viral disease, using histopathology and ultrastructural pathology, nested PCR and back to a sense of means test and other methods, the virus pathogen identified RNA, ss RNA, WSSV, reovirus. In the study of bacterial disease, using bacterial isolation and culture, biological characteristics, pathogenicity and drug sensitivity analysis method,to determine the bacterial pathogens with Aeromonas, Vibrio, Citrobacter spp, Aeromonas punctata, Vivtorinox Aeromonas. In the study of parasitic disease, using pathological section,microscope and other technical means to determine the parasitic disease pathogenic microsporidia,barnacles, polydora ligni, sessile ciliates. In non biological pathogenic factors, the theories and methods of ecology, organization of heavy metals and nutrient and water drainage. To prevention and treatment, immunological studies were carried out, and the drug sensitivity, toxicity and immune experiment were studied from the view of medicine and immunology.
引文