The Abortion Mechanism and Fertility Restoration of Microspores during the Anther Development in Cotton Genic Male Sterile Line Yu 98-8A
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- 英文论文题名：The Abortion Mechanism and Fertility Restoration of Microspores during the Anther Development in Cotton Genic Male Sterile Line Yu 98-8A
- 论文作者：Yang Xiaojie ; Liu Ji ; Tang Zhongjie ; Sun Yao ; Zhao Yuanming ; Li Wu ; Xie Deyi ; Fang Weiping
- 英文论文作者：Yang Xiaojie ; Liu Ji ; Tang Zhongjie ; Sun Yao ; Zhao Yuanming ; Li Wu ; Xie Deyi ; Fang Weiping ; Industrial Crop Research Institute ; Henan Academy of Agricultural Sciences ; State Key Laboratory of Cotton Biology/Institute of Cotton Research of CAAS
- 年：2017
- 作者机构：Industrial Crop Research Institute,Henan Academy of Agricultural Sciences;State Key Laboratory of Cotton Biology/Institute of Cotton Research of CAAS;
- 英文论文关键词：cotton ; male sterile ; abortion mechanism ; fertility restoration
- 会议召开时间：2017-08-07
- 会议录名称：中国农学会棉花分会2017年年会暨第九次会员代表大会论文汇编
- 语种：英文
- 分类号：S562
- 学会代码：MHXB
- 会议名称：中国农学会棉花分会2017年年会暨第九次会员代表大会
- 会议地点：中国河南郑州
- 主办单位：中国农学会棉花分会
- 学会名称：《棉花学报》编辑部
- 页数：1
- 文件大小：39k
- 原文格式：O
- 会议级别：全国
摘要
Although cotton genic male sterility(GMS) plays an important role in the utilization of hybrid vigor,its precise molecular mechanism remains unclear.To characterize the molecular events of microspore abortion and fertility restoration,transcriptome,microRNA expression analysis combined with histological observations and hormones treatments were conducted in the cotton GMS line Yu 98-8A.Histological analysis showed the shriveled tetrad was the first significant microscopic phenotype that developed,indicating that the pollen mother cell formation phase and the following meiotic cycle are vital periods of microspores abortion in MS of Yu 98-8A.Biochemical transcriptome bioinformatic analysis indicated that the differentially expressed genes(DEGs) mainly involved in sugar and starch metabolism,oxidative phosphorylation,and phytohormones might play a critical and complicated role in pollen abortion.At the same time,microRNA expression analysis also showed indole-3-acetic acid(IAA) and gibberellic acid(GA) signalling tranduction pathways may play pivotal regulatory function in male sterility.Further studies have shown that regulating endogenous GA3 content in young buds restore the fertility of Yu 98-8A effectively.These results provide an important strategy of overcoming the bottleneck in cotton heterosis utilization for us to avoid the disadvantages(removal of about 50% fertile plants) of cotton genic male sterile lines effectively.
Although cotton genic male sterility(GMS) plays an important role in the utilization of hybrid vigor,its precise molecular mechanism remains unclear.To characterize the molecular events of microspore abortion and fertility restoration,transcriptome,microRNA expression analysis combined with histological observations and hormones treatments were conducted in the cotton GMS line Yu 98-8A.Histological analysis showed the shriveled tetrad was the first significant microscopic phenotype that developed,indicating that the pollen mother cell formation phase and the following meiotic cycle are vital periods of microspores abortion in MS of Yu 98-8A.Biochemical transcriptome bioinformatic analysis indicated that the differentially expressed genes(DEGs) mainly involved in sugar and starch metabolism,oxidative phosphorylation,and phytohormones might play a critical and complicated role in pollen abortion.At the same time,microRNA expression analysis also showed indole-3-acetic acid(IAA) and gibberellic acid(GA) signalling tranduction pathways may play pivotal regulatory function in male sterility.Further studies have shown that regulating endogenous GA3 content in young buds restore the fertility of Yu 98-8A effectively.These results provide an important strategy of overcoming the bottleneck in cotton heterosis utilization for us to avoid the disadvantages(removal of about 50% fertile plants) of cotton genic male sterile lines effectively.
Although cotton genic male sterility(GMS) plays an important role in the utilization of hybrid vigor,its precise molecular mechanism remains unclear.To characterize the molecular events of microspore abortion and fertility restoration,transcriptome,microRNA expression analysis combined with histological observations and hormones treatments were conducted in the cotton GMS line Yu 98-8A.Histological analysis showed the shriveled tetrad was the first significant microscopic phenotype that developed,indicating that the pollen mother cell formation phase and the following meiotic cycle are vital periods of microspores abortion in MS of Yu 98-8A.Biochemical transcriptome bioinformatic analysis indicated that the differentially expressed genes(DEGs) mainly involved in sugar and starch metabolism,oxidative phosphorylation,and phytohormones might play a critical and complicated role in pollen abortion.At the same time,microRNA expression analysis also showed indole-3-acetic acid(IAA) and gibberellic acid(GA) signalling tranduction pathways may play pivotal regulatory function in male sterility.Further studies have shown that regulating endogenous GA3 content in young buds restore the fertility of Yu 98-8A effectively.These results provide an important strategy of overcoming the bottleneck in cotton heterosis utilization for us to avoid the disadvantages(removal of about 50% fertile plants) of cotton genic male sterile lines effectively.
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