Arabidopsis MMD1/DUET ensures the progression of male meiotic chromosome condensation and directly regulates the expression of condensin gene CAP-D3
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- 英文论文题名：Arabidopsis MMD1/DUET ensures the progression of male meiotic chromosome condensation and directly regulates the expression of condensin gene CAP-D3
- 论文作者：Jun Wang ; Baixiao Niu ; Jiyue Huang ; Hongkuan Wang ; Xiaohui Yang ; Aiwu Dong ; Christopher Makaroff ; Hong Ma ; Yingxiang Wang
- 英文论文作者：Jun Wang ; Baixiao Niu ; Jiyue Huang ; Hongkuan Wang ; Xiaohui Yang ; Aiwu Dong ; Christopher Makaroff ; Hong Ma ; Yingxiang Wang ; State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development ; Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering and Institute of Biodiversity Sciences ; Institute of Plants Biology ; Center for Evolutionary Biology ; School of Life Sciences ; Fudan University ; Department of Chemistry and Biochemistry ; Miami University ; Institutes of Biomedical Sciences ; Fudan University
- 年：2016
- 作者机构：State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development,Ministry of Education Key Laboratory of Biodiversity Science and Ecological Engineering and Institute of Biodiversity Sciences,Institute of Plants Biology,Center for Evolutionary Biology,School of Life Sciences,Fudan University;Department of Chemistry and Biochemistry,Miami University;Institutes of Biomedical Sciences,Fudan University;
- 英文论文关键词：MMD1 ; meiotic chromosome condensation ; condensin
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Chromosome condensation mediated by the condensin complex is essential for proper chromosome segregation during cell division. Unlike the rapid mitotic chromosome condensation,meiotic chromosome condensation occurs over a relative long prophase I and is unusually complex due to the coordination with chromosome axis formation and homolog interaction. The molecular mechanisms that regulate meiotic chromosome condensation progression from prophase I to metaphase I are unclear. We show here that the Arabidopsis meiotic PHD-finger protein MMD1/DUET is required for progressive compaction of prophase I chromosomes to metaphase I bivalents. The MMD1 PHD domain is required for its function in chromosome condensation and binds to methylated histone tails. Transcriptome analysis and q RT-PCR showed that several condensin genes exhibit significantly reduced expression in mmd1 meiocytes. Furthermore,MMD1 specifically binds to the promoter region of the condensin subunit gene CAP-D3 to enhance its expression. Moreover,capd3 mutants exhibit similar chromosome condensation defects,revealing a novel MMD1-dependent mechanism for regulating meiotic chromosome condensation in part by promoting condensin gene expression. Together,these discoveries provide strong evidence that the histone reader MMD1/DUET defines an important step for regulating the progression of meiotic prophase I chromosome condensation.
Chromosome condensation mediated by the condensin complex is essential for proper chromosome segregation during cell division. Unlike the rapid mitotic chromosome condensation,meiotic chromosome condensation occurs over a relative long prophase I and is unusually complex due to the coordination with chromosome axis formation and homolog interaction. The molecular mechanisms that regulate meiotic chromosome condensation progression from prophase I to metaphase I are unclear. We show here that the Arabidopsis meiotic PHD-finger protein MMD1/DUET is required for progressive compaction of prophase I chromosomes to metaphase I bivalents. The MMD1 PHD domain is required for its function in chromosome condensation and binds to methylated histone tails. Transcriptome analysis and q RT-PCR showed that several condensin genes exhibit significantly reduced expression in mmd1 meiocytes. Furthermore,MMD1 specifically binds to the promoter region of the condensin subunit gene CAP-D3 to enhance its expression. Moreover,capd3 mutants exhibit similar chromosome condensation defects,revealing a novel MMD1-dependent mechanism for regulating meiotic chromosome condensation in part by promoting condensin gene expression. Together,these discoveries provide strong evidence that the histone reader MMD1/DUET defines an important step for regulating the progression of meiotic prophase I chromosome condensation.
Chromosome condensation mediated by the condensin complex is essential for proper chromosome segregation during cell division. Unlike the rapid mitotic chromosome condensation,meiotic chromosome condensation occurs over a relative long prophase I and is unusually complex due to the coordination with chromosome axis formation and homolog interaction. The molecular mechanisms that regulate meiotic chromosome condensation progression from prophase I to metaphase I are unclear. We show here that the Arabidopsis meiotic PHD-finger protein MMD1/DUET is required for progressive compaction of prophase I chromosomes to metaphase I bivalents. The MMD1 PHD domain is required for its function in chromosome condensation and binds to methylated histone tails. Transcriptome analysis and q RT-PCR showed that several condensin genes exhibit significantly reduced expression in mmd1 meiocytes. Furthermore,MMD1 specifically binds to the promoter region of the condensin subunit gene CAP-D3 to enhance its expression. Moreover,capd3 mutants exhibit similar chromosome condensation defects,revealing a novel MMD1-dependent mechanism for regulating meiotic chromosome condensation in part by promoting condensin gene expression. Together,these discoveries provide strong evidence that the histone reader MMD1/DUET defines an important step for regulating the progression of meiotic prophase I chromosome condensation.
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