Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced antiviral therapeutic effects
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- 英文论文题名：Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced antiviral therapeutic effects
- 论文作者：Zhan Linsheng ; Zhou Qianqian ; Zhang Yulong ; Wang Xiaohui
- 英文论文作者：Zhan Linsheng ; Zhou Qianqian ; Zhang Yulong ; Wang Xiaohui ; Beijing transfusion institute
- 年：2016
- 作者机构：Beijing transfusion institute;
- 英文论文关键词：DCs ; cryopreservation ; migration ; bioluminescence imaging ; anti-viral effects
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：341k
- 原文格式：D
- 会议级别：全国
摘要
Objective: Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell(DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DC matured from cryopreserved immature DCs(cryoim-m DCs) remain to be revealed.Methods: In the present study, we compared cryoim-m DCs with DCs matured from fresh immature DCs(fm DCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. Results: The results show that cryopreservation impair the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8~+ T cell response induced by cryoim-m DCs was much weaker than that induced by fm DCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. Conclusions: In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DC matured from frozen immature DCs were hindered to some extent.
Objective: Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell(DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DC matured from cryopreserved immature DCs(cryoim-m DCs) remain to be revealed.Methods: In the present study, we compared cryoim-m DCs with DCs matured from fresh immature DCs(fm DCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. Results: The results show that cryopreservation impair the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8+ T cell response induced by cryoim-m DCs was much weaker than that induced by fm DCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. Conclusions: In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DC matured from frozen immature DCs were hindered to some extent.
Objective: Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell(DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DC matured from cryopreserved immature DCs(cryoim-m DCs) remain to be revealed.Methods: In the present study, we compared cryoim-m DCs with DCs matured from fresh immature DCs(fm DCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. Results: The results show that cryopreservation impair the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8+ T cell response induced by cryoim-m DCs was much weaker than that induced by fm DCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. Conclusions: In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DC matured from frozen immature DCs were hindered to some extent.
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