CCAR1 5′UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance
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- 英文论文题名：CCAR1 5′UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance
- 论文作者：Gaopeng Li ; Xiaoli Wu ; Wenchang Qian ; Huayong Cai ; Xinbao Sun ; Weijie Zhang ; Sheng Tan ; Zhengsheng Wu ; Pengxu Qian ; Keshuo Ding ; Xuefei Lu ; Xiao Zhang ; Hong Yan ; Haifeng Song ; Shouhong Guang ; Qingfa Wu ; Peter E Lobie ; Ge Shan ; 朱涛
- 英文论文作者：Gaopeng Li ; Xiaoli Wu ; Wenchang Qian ; Huayong Cai ; Xinbao Sun ; Weijie Zhang ; Sheng Tan ; Zhengsheng Wu ; Pengxu Qian ; Keshuo Ding ; Xuefei Lu ; Xiao Zhang ; Hong Yan ; Haifeng Song ; Shouhong Guang ; Qingfa Wu ; Peter E Lobie ; Ge Shan ; Tao Zhu ; The CAS Key Laboratory of Innate Immunity and Chronic Disease ; School of Life Sciences ; University of Science and Technology of China ; Hefei National Laboratory for Physical Sciences at Microscale ; Department of Pathology ; Anhui Medical University ; Stowers Institute for Medical Research ; Department of Pharmacology and Toxicology ; Beijing Institute of Radiation Medicine ; Cancer Science Institute of Singapore and Department of Pharmacology ; National University of Singapore
- 年：2016
- 作者机构：中国科学技术大学;Hefei National Laboratory for Physical Sciences at Microscale;Department of Pathology,Anhui Medical University;Stowers Institute for Medical Research;Department of Pharmacology and Toxicology,Beijing Institute of Radiation Medicine;Cancer Science Institute of Singapore and Department of Pharmacology,National University of Singapore;
- 英文论文关键词：CCAR1 5'UTR ; miR-1254 ; mi Rancer ; tamoxifen
- 会议召开时间：2016-09-25
- 会议录名称：第九届全国核糖核酸学术讨论会论文集
- 语种：英文
- 分类号：Q78
- 学会代码：IGSS
- 会议名称：第九届全国核糖核酸学术讨论会
- 会议地点：中国上海
- 主办单位：中国生物化学与分子生物学会核糖核酸专业委员会
- 学会名称：中国生物化学与分子生物学会
- 页数：1
- 文件大小：602k
- 原文格式：D
- 会议级别：全国
摘要
MicroRNAs(miRNAs)typically bind to unstructured miRNA-binding sites in targetRNAs,leading to a mutual repression of expression.Here,we report that miR-1254interacts with structured elements in cell cycle and apoptosis regulator 1(CCAR1)5′untranslated region(UTR)and this interaction enhances the stability of both molecules.miR-1254 can also act as a repressor when binding to unstructured sites in its targets.Interestingly,structured miR-1254-targeting sites act as both a functional RNA motifsensing unit,and an independentRNA functional unit that enhances miR-1254expression.Artificially designed miRNA enhancers,termed"miRancers",can stabilize and enhance the activity of miRNAs of interest.We further demonstrate that CCAR1 5′UTR as a natural miRancer of endogenous miR-1254 re-sensitizes tamoxifen-resistant breast cancer cells to tamoxifen.Thus,our study presents a novel model of miRNA function,wherein highly structured miRancer-like motif-containing RNA fragments or miRancer molecules specifically interact with miRNAs,leading to reciprocal stabilization.
MicroRNAs(miRNAs) typically bind to unstructured mi RNA-binding sites in targetRNAs,leading to a mutual repression of expression.Here,we report that mi R-1254 interacts with structured elements in cell cycle and apoptosis regulator 1(CCAR1) 5′ untranslated region(UTR) and this interaction enhances the stability of both molecules.mi R-1254 can also act as a repressor when binding to unstructured sites in its targets.Interestingly,structured mi R-1254-targeting sites act as both a functional RNA motifsensing unit,and an independentRNA functional unit that enhances mi R-1254 expression.Artificially designed mi RNA enhancers,termed "mi Rancers",can stabilize and enhance the activity of mi RNAs of interest.We further demonstrate that CCAR1 5′ UTR as a natural mi Rancer of endogenous mi R-1254 re-sensitizes tamoxifen-resistant breast cancer cells to tamoxifen.Thus,our study presents a novel model of mi RNA function,wherein highly structured mi Rancer-like motif-containing RNA fragments or mi Rancer molecules specifically interact with mi RNAs,leading to reciprocal stabilization.
MicroRNAs(miRNAs) typically bind to unstructured mi RNA-binding sites in targetRNAs,leading to a mutual repression of expression.Here,we report that mi R-1254 interacts with structured elements in cell cycle and apoptosis regulator 1(CCAR1) 5′ untranslated region(UTR) and this interaction enhances the stability of both molecules.mi R-1254 can also act as a repressor when binding to unstructured sites in its targets.Interestingly,structured mi R-1254-targeting sites act as both a functional RNA motifsensing unit,and an independentRNA functional unit that enhances mi R-1254 expression.Artificially designed mi RNA enhancers,termed "mi Rancers",can stabilize and enhance the activity of mi RNAs of interest.We further demonstrate that CCAR1 5′ UTR as a natural mi Rancer of endogenous mi R-1254 re-sensitizes tamoxifen-resistant breast cancer cells to tamoxifen.Thus,our study presents a novel model of mi RNA function,wherein highly structured mi Rancer-like motif-containing RNA fragments or mi Rancer molecules specifically interact with mi RNAs,leading to reciprocal stabilization.
引文