Tuning growth cycles of Brassica crops via natural antisense transcripts of BrFLC
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- 英文论文题名：Tuning growth cycles of Brassica crops via natural antisense transcripts of BrFLC
- 论文作者：Xiaorong Li ; Shaofeng Zhang ; Jinjuan Bai ; Yajie Li ; Yuke He
- 英文论文作者：Xiaorong Li ; Shaofeng Zhang ; Jinjuan Bai ; Yajie Li ; Yuke He ; National Key Laboratory of Plant Molecular Genetics ; Shanghai Institute of Plant Physiology and Ecology ; Shanghai Institutes for Biological Sciences ; Chinese Academy of Sciences
- 年：2016
- 作者机构：National Key Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences;
- 英文论文关键词：Brassica rapa ; BrFLC ; natural antisense transcripts ; flowering time ; growth cycle ; vernalization
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：841k
- 原文格式：D
- 会议级别：全国
摘要
Several oilseed and vegetable crops of Brassica are biennials that require a prolonged winter cold for flowering,a process called vernalization. FLOWERING LOCUS C(FLC) is a central repressor of flowering. Here,we report that the overexpression of natural antisense transcripts(NATs) of Brassica rapa FLC(Br FLC) greatly shortens plant growth cycles. In rapid-,medium-and slow-cycling crop types,there are four copies of the BrFLC genes,which show extensive variation in sequences and expression levels. In Bre,a biennial crop type that requires vernalization,five NATs derived from the BrFLC2 locus are rapidly induced under cold conditions,while all four BrFLC genes are gradually down-regulated. The transgenic Bre lines overexpressing a long NAT of BrFLC2 do not require vernalization,resulting in a gradient of shortened growth cycles. Among them,a subset of lines both flower and set seeds as early as Yellow sarson,an annual crop type in which all four BrFLC genes have nonsense mutations and are non-functional in flowering repression. Our results demonstrate that the growth cycles of biennial crops of Brassica can be altered by changing the expression levels of BrFLC2 NATs. Thus,BrFLC2 NATs and their transgenic lines are useful for the genetic manipulation of crop growth cycles.
Several oilseed and vegetable crops of Brassica are biennials that require a prolonged winter cold for flowering,a process called vernalization. FLOWERING LOCUS C(FLC) is a central repressor of flowering. Here,we report that the overexpression of natural antisense transcripts(NATs) of Brassica rapa FLC(Br FLC) greatly shortens plant growth cycles. In rapid-,medium-and slow-cycling crop types,there are four copies of the BrFLC genes,which show extensive variation in sequences and expression levels. In Bre,a biennial crop type that requires vernalization,five NATs derived from the BrFLC2 locus are rapidly induced under cold conditions,while all four BrFLC genes are gradually down-regulated. The transgenic Bre lines overexpressing a long NAT of BrFLC2 do not require vernalization,resulting in a gradient of shortened growth cycles. Among them,a subset of lines both flower and set seeds as early as Yellow sarson,an annual crop type in which all four BrFLC genes have nonsense mutations and are non-functional in flowering repression. Our results demonstrate that the growth cycles of biennial crops of Brassica can be altered by changing the expression levels of BrFLC2 NATs. Thus,BrFLC2 NATs and their transgenic lines are useful for the genetic manipulation of crop growth cycles.
Several oilseed and vegetable crops of Brassica are biennials that require a prolonged winter cold for flowering,a process called vernalization. FLOWERING LOCUS C(FLC) is a central repressor of flowering. Here,we report that the overexpression of natural antisense transcripts(NATs) of Brassica rapa FLC(Br FLC) greatly shortens plant growth cycles. In rapid-,medium-and slow-cycling crop types,there are four copies of the BrFLC genes,which show extensive variation in sequences and expression levels. In Bre,a biennial crop type that requires vernalization,five NATs derived from the BrFLC2 locus are rapidly induced under cold conditions,while all four BrFLC genes are gradually down-regulated. The transgenic Bre lines overexpressing a long NAT of BrFLC2 do not require vernalization,resulting in a gradient of shortened growth cycles. Among them,a subset of lines both flower and set seeds as early as Yellow sarson,an annual crop type in which all four BrFLC genes have nonsense mutations and are non-functional in flowering repression. Our results demonstrate that the growth cycles of biennial crops of Brassica can be altered by changing the expression levels of BrFLC2 NATs. Thus,BrFLC2 NATs and their transgenic lines are useful for the genetic manipulation of crop growth cycles.
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