烟草PVY隐性抗病基因的分子标记及其适用性
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- 英文论文题名：Molecular Marker from Recessive Resistance Gene to Potato virus Y of Tobacco and its Suitability
- 论文作者：刘勇 ; 宋中邦 ; 童治军 ; 李永平
- 英文论文作者：Liu Yong ; Song Zhong-bang ; Tong Zhi-jun ; Li Yong-ping ; Yunnan Academy of Tobacco Agricultural Sciences ; Key Laboratory of Tobacco Biotechnological Breeding ; National Tobacco Genetic Engineering Research Center
- 年：2016
- 作者机构：云南省烟草农业科学研究院烟草行业烟草生物技术育种重点实验室国家烟草基因工程研究中心;
- 论文关键词：烟草 ; 马铃薯Y病毒 ; 抗性 ; 分子标记
- 英文论文关键词：tobacco ; potato virus Y ; resistance ; molecular marker
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草农业主题
- 语种：中文
- 分类号：S435.72
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：9
- 文件大小：676k
- 原文格式：D
摘要
马铃薯Y病毒(Potato virus Y,PVY)是危害烟草的重要病害,种植抗病品种是经济有效的防治措施。分子标记辅助选择可提高抗病育种效率。来源于X-射线诱变的Virgin A Mutante(VAM)的隐性抗PVY基因位点(va)被广泛应用于烟草抗病育种。为了提高va位点的育种利用效率,根据烟草隐性抗PVY基因(感病基因)e IF4E-1基因序列,设计特异扩增的引物CF2GR11,开发e IF4E-1基因的分子标记,并检测了该标记与抗性的遗传距离和在常见烟草资源中的适用性。CF2GR11在云烟87、红花大金元和K326等感PVY品种可扩增出500 bp产物,在NC102、NC55和K326PVY等抗病品种无扩增条带。以抗、感PVY亲本构建的101个F2单株为定位群体,遗传连锁分析表明,CF2GR11标记与烟草PVY感病基因的遗传距离为0.99 c M。对46份PVY抗性明确的栽培烟草资源的检测表明,供试资源的标记检测结果与抗性的吻合度为100%,表明CF2GR11标记适用性高。该目的基因标记可用于抗PVY育种的辅助选择和抗PVY资源的鉴定。
Potato virus Y(PVY) is a serious disease of tobacco, use of resistant varieties are economical and effective control measures. Molecular marker-assisted selection could improve breeding efficiency. A gene locus va of Virgin A Mutante(VAM), an X-ray irradiation-induced recessive resistance source is widely used in tobacco breeding. In order to improve va locus breeding utilization efficiency, A specific amplification primer CF2GR11 was developed based on recessive resistance gene e IF4E-1(Susceptible gene) to PVY of tobacco, the genetic distance between CF2GR11 and resistance, the suitability of CF2GR11 in tobacco germplasm were evaluated. In PVY susceptible varieties Yunyan87, Honghuadajinyuan and K326, CF2GR11 can amplify a 446 bp product, while in PVY resistant varieties NC102, NC55 and K326 PVY no product came out. Genotyping PVY resistance segregated F2 population come from a PVY resistant and a susceptible parent, the genetic distance between CF2GR11 marker and resistance is 0.99 c M. CF2GR11 is suitable in 46 tested tobacco germplasm. This gene targeted marker could use as an assisted selection tool for PVY resistant cultivars breeding and for germplasm identification.
Potato virus Y(PVY) is a serious disease of tobacco, use of resistant varieties are economical and effective control measures. Molecular marker-assisted selection could improve breeding efficiency. A gene locus va of Virgin A Mutante(VAM), an X-ray irradiation-induced recessive resistance source is widely used in tobacco breeding. In order to improve va locus breeding utilization efficiency, A specific amplification primer CF2GR11 was developed based on recessive resistance gene e IF4E-1(Susceptible gene) to PVY of tobacco, the genetic distance between CF2GR11 and resistance, the suitability of CF2GR11 in tobacco germplasm were evaluated. In PVY susceptible varieties Yunyan87, Honghuadajinyuan and K326, CF2GR11 can amplify a 446 bp product, while in PVY resistant varieties NC102, NC55 and K326 PVY no product came out. Genotyping PVY resistance segregated F2 population come from a PVY resistant and a susceptible parent, the genetic distance between CF2GR11 marker and resistance is 0.99 c M. CF2GR11 is suitable in 46 tested tobacco germplasm. This gene targeted marker could use as an assisted selection tool for PVY resistant cultivars breeding and for germplasm identification.
引文
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[10]王贵,刘勇,卢秀萍,等.烟草PVY抗性的遗传分析与分子标记筛选[J].分子植物育种,2012,10(1):97-103.
[11]Chikara M,Hiroshi M,Tatsuji H,et al.A single amino acid change in viral genome-associated protein of potato virus Y correlates with resistance breeding in“Virgin A Mutant”tobacco[J].Phytopathology,1999,89(2):118-123.
[12]林志文,刘勇,李梅云,等.烟草种质资源抗马铃薯Y病毒病鉴定方法比较[J].中国农学通报2010,26(19):269-274.
[13]Murry HG,Thomspon WF.Rapid isolation of weight DNA[J].Nucleic Acids Res,1980,8:4321-4322.
[14]Tajima T,Noguchi S,Tanoue W,et al.Background selection using DNA markers in backcross breeding program for potato virus Y resistance of tobacco[J].Breeding Science,2002,52:253-257.
[15]肖炳光,高玉龙,吴为人.23份烟草品种遗传关系的SSR分析[J].分子植物育种(网络版),2011,4(9):1297-1304.
[16]Kosambi DD.The estimation of map distances from recombination values[J].Ann Eugen,1944,12:172-175.
[17]Lewis R S.Transfer of resistance to potato virus Y(PVY)from Nicotiana Africana to Nicotiana tabacum:possible influence of tissue culture on the rate of introgression[J].Theor Appl Genet,2005,110:678-687.
[18]Acosta-Leal R,Xiong Z.Complementary functions of two recessive R-genes determine resistance durability of tobacco'Virgin A Mutant'(VAM)to Potato virus Y[J].Virology,2008,379(2):275-283.
[2]李义强,王凤龙,程云吉,等.黄淮烟区烟草病毒病发生种类、发病规律研究,中国烟叶学术论文集,科学技术文献出版社,北京:2004:438-442.
[3]刘勇,莫笑晗,余清,等.云南、福建、湖南烟区烟草花叶病主要病毒种类检测及黄瓜花叶病毒亚组鉴定[J].植物病理学报,2006,36(4):310-313.
[4]Burk L.G.,Gooding G.V.,and Chaplin J.F.Reaction of Nicotiana species and cultivars or breeding lines of Nicotiana tabacum to three strains of potato virus Y[J].Tobacco Science,1982,26:85-88.
[5]陈荣平,焦庆明,兰荣利,等.烟草抗PVY育种材料的筛选与应用[J].中国烟草科学,2000(1):1-4.
[6]周显升,钱玉梅,陈德鑫,等.烟草品种对马铃薯Y病毒的抗性遗传分析[J].中国烟草学报[J].2005,13(1):31-36.
[7]陈万胜.烟草马铃薯Y病毒病抗性遗传及分子标记[D],中国农业科学院研究生院,2008.
[8]Noguchi S,Tajima T,Yamamoto Y,et al.Deletion of a large genomic segment in tobacco varieties that are resistant to potato virus Y(PVY)[J].Mol Gen Genet,1999,262:822-829.
[9]Julio E,Denoyes-Rothan B,Verrier JL,et al.Detection of QTLs linked to leaf and smoke properties in Nicotiana tabacum based on a study of 114 recombinant inbred lines[J].Mol Breeding,2006,14:114-123.
[10]王贵,刘勇,卢秀萍,等.烟草PVY抗性的遗传分析与分子标记筛选[J].分子植物育种,2012,10(1):97-103.
[11]Chikara M,Hiroshi M,Tatsuji H,et al.A single amino acid change in viral genome-associated protein of potato virus Y correlates with resistance breeding in“Virgin A Mutant”tobacco[J].Phytopathology,1999,89(2):118-123.
[12]林志文,刘勇,李梅云,等.烟草种质资源抗马铃薯Y病毒病鉴定方法比较[J].中国农学通报2010,26(19):269-274.
[13]Murry HG,Thomspon WF.Rapid isolation of weight DNA[J].Nucleic Acids Res,1980,8:4321-4322.
[14]Tajima T,Noguchi S,Tanoue W,et al.Background selection using DNA markers in backcross breeding program for potato virus Y resistance of tobacco[J].Breeding Science,2002,52:253-257.
[15]肖炳光,高玉龙,吴为人.23份烟草品种遗传关系的SSR分析[J].分子植物育种(网络版),2011,4(9):1297-1304.
[16]Kosambi DD.The estimation of map distances from recombination values[J].Ann Eugen,1944,12:172-175.
[17]Lewis R S.Transfer of resistance to potato virus Y(PVY)from Nicotiana Africana to Nicotiana tabacum:possible influence of tissue culture on the rate of introgression[J].Theor Appl Genet,2005,110:678-687.
[18]Acosta-Leal R,Xiong Z.Complementary functions of two recessive R-genes determine resistance durability of tobacco'Virgin A Mutant'(VAM)to Potato virus Y[J].Virology,2008,379(2):275-283.