Effects of Bacillus licheniformis on the growth performance and expression of lipid metabolism-related genes in broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis
详细信息 查看官网全文
- 英文论文题名：Effects of Bacillus licheniformis on the growth performance and expression of lipid metabolism-related genes in broiler chickens challenged with Clostridium perfringens-induced necrotic enteritis
- 论文作者：Mengjia Zhou ; Dong Zeng ; Xueqin Ni ; Teng Tu ; Zhongqiong Yin ; Kangcheng Pan ; Bo Jing
- 英文论文作者：Mengjia Zhou ; Dong Zeng ; Xueqin Ni ; Teng Tu ; Zhongqiong Yin ; Kangcheng Pan ; Bo Jing ; Animal Microecology Institute ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province ; Animal Genetics and Breeding Institute ; College of Animal Science and technology ; Sichuan Agricultural University
- 年：2016
- 作者机构：Animal Microecology Institute, College of Veterinary Medicine,Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province;Animal Genetics and Breeding Institute, College of Animal Science and technology, Sichuan Agricultural University;
- 英文论文关键词：broiler chicken ; necrotic enteritis ; growth performance ; antioxidant ; lipid metabolism
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：英文
- 分类号：S831.5
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：73k
- 原文格式：O
- 会议级别：全国
摘要
Background:Necrotic enteritis(NE),caused by Clostridium perfringens,has cost the poultry industry $2billion in losses.This study aimed to investigate the effect of Bacillus licheniformis as dietary supplement on the growth,serum antioxidant status,and expression of lipid-metabolism genes of broiler chickens with C.perfringens-induced NE.Methods:A total of 240 one-day-old broilers were randomly grouped into four:a negative control,an NE experimental model(PC),chickens fed a diet supplemented with 30%of fishmeal from day 14 onwards and challenged with coccidiosis vaccine(FC),and NE group supplied with feed containing 1.0×10~6 CFU/g B.licheniformis(BL).Results:Body weight gain,feed conversion ratio,serum antioxidant status,and lipid-metabolism-gene expression were analyzed.In the PC group,FCR increased significantly whereas serum catalase and glutathione peroxidase activity decreased compared with NC group.Dietary B.licheniformis supplementation improved FCR and oxidative stress in experimental avian NE.Using Bacillus licheniformis as a direct-fed microbial(DFM) could also significantly upregulate catabolism-related genes,namely,peroxisome proliferator-activated receptor-α and carnitine palmitoyltransferase-1,in livers and changed the expression of lipid-anabolism genes.Conclusion:These results suggested that dietary B.licheniformis supplementation can enhance growth and antioxidant ability,as well as change the expression of genes related to fatty-acid synthesis and oxidation in the livers of NE-infected broilers.
Background:Necrotic enteritis(NE),caused by Clostridium perfringens,has cost the poultry industry $2billion in losses.This study aimed to investigate the effect of Bacillus licheniformis as dietary supplement on the growth,serum antioxidant status,and expression of lipid-metabolism genes of broiler chickens with C.perfringens-induced NE.Methods:A total of 240 one-day-old broilers were randomly grouped into four:a negative control,an NE experimental model(PC),chickens fed a diet supplemented with 30%of fishmeal from day 14 onwards and challenged with coccidiosis vaccine(FC),and NE group supplied with feed containing 1.0×10~6 CFU/g B.licheniformis(BL).Results:Body weight gain,feed conversion ratio,serum antioxidant status,and lipid-metabolism-gene expression were analyzed.In the PC group,FCR increased significantly whereas serum catalase and glutathione peroxidase activity decreased compared with NC group.Dietary B.licheniformis supplementation improved FCR and oxidative stress in experimental avian NE.Using Bacillus licheniformis as a direct-fed microbial(DFM) could also significantly upregulate catabolism-related genes,namely,peroxisome proliferator-activated receptor-α and carnitine palmitoyltransferase-1,in livers and changed the expression of lipid-anabolism genes.Conclusion:These results suggested that dietary B.licheniformis supplementation can enhance growth and antioxidant ability,as well as change the expression of genes related to fatty-acid synthesis and oxidation in the livers of NE-infected broilers.
Background:Necrotic enteritis(NE),caused by Clostridium perfringens,has cost the poultry industry $2billion in losses.This study aimed to investigate the effect of Bacillus licheniformis as dietary supplement on the growth,serum antioxidant status,and expression of lipid-metabolism genes of broiler chickens with C.perfringens-induced NE.Methods:A total of 240 one-day-old broilers were randomly grouped into four:a negative control,an NE experimental model(PC),chickens fed a diet supplemented with 30%of fishmeal from day 14 onwards and challenged with coccidiosis vaccine(FC),and NE group supplied with feed containing 1.0×10~6 CFU/g B.licheniformis(BL).Results:Body weight gain,feed conversion ratio,serum antioxidant status,and lipid-metabolism-gene expression were analyzed.In the PC group,FCR increased significantly whereas serum catalase and glutathione peroxidase activity decreased compared with NC group.Dietary B.licheniformis supplementation improved FCR and oxidative stress in experimental avian NE.Using Bacillus licheniformis as a direct-fed microbial(DFM) could also significantly upregulate catabolism-related genes,namely,peroxisome proliferator-activated receptor-α and carnitine palmitoyltransferase-1,in livers and changed the expression of lipid-anabolism genes.Conclusion:These results suggested that dietary B.licheniformis supplementation can enhance growth and antioxidant ability,as well as change the expression of genes related to fatty-acid synthesis and oxidation in the livers of NE-infected broilers.
引文
Songer JG,Meer RR:Genotyping ofClostridium perfringensby Polymerase Chain Reaction is a Useful Adjunct to Diagnosis of Clostridial Enteric Disease in Animals.Anaerobe 1996,2:197-203
Immerseel FV,Buck JD,Pasmans F,Huyghebaert G,Haesebrouck F,Ducatelle R:Clostridium perfringens in poultry:an emerging threat for animal and public health.Avian pathology 2004,33:537-549.
McDevitt R,Brooker J,Acamovic T,Sparks N:Necrotic enteritis;a continuing challenge for the poultry industry.World's Poultry Science Journal 2006,62:221-247
Immerseel FV,Buck JD,Pasmans F,Huyghebaert G,Haesebrouck F,Ducatelle R:Clostridium perfringens in poultry:an emerging threat for animal and public health.Avian pathology 2004,33:537-549.
McDevitt R,Brooker J,Acamovic T,Sparks N:Necrotic enteritis;a continuing challenge for the poultry industry.World's Poultry Science Journal 2006,62:221-247