Dynamic epigenome of differentiation and maturation of human dendritic cells
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- 英文论文题名：Dynamic epigenome of differentiation and maturation of human dendritic cells
- 论文作者：Zhang Qian ; Shen Qicong ; Han Yanmei ; Cao Xuetao
- 英文论文作者：Zhang Qian ; Shen Qicong ; Han Yanmei ; Cao Xuetao ; National Key Laboratory of Medical Immunology & Institute of Immunology ; Second Military Medical University
- 年：2016
- 作者机构：National Key Laboratory of Medical Immunology & Institute of Immunology,Second Military Medical University;
- 英文论文关键词：Epigenomics ; Dendritic cells ; DNA methylation ; Histone modifications
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R392
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1295k
- 原文格式：D
- 会议级别：全国
摘要
Objective: Combinatorial epigenetic regulationis critical to maintain unique cell identity. However, dynamic epigenomes of human primary cells, particularly immune cell lineages undergoing differentiation, have rarely been reported. Methods: Using human primary lineage of monocytes, monocyte-derived immature dendritic cells(im DCs) and LPSmatured DCs(m DCs) representing the dynamic process of antigen-presenting cell differentiation and maturation, we established base-resolution DNA methylomes, H3K4me3 and H3K27me3 maps and mRNA transcriptome of this dynamic lineage.Results: Comparative analysis of dynamic epigenomic patterns revealed that DNA demethylation during monocytes differentiation into im DCs and de novo methylation during DCs maturation are DC-dominant DNA methylation variations. These genome-wide differential DNA methylation regions(DMRs) are mostly outside CpG islands. Conclusion: For histone modifications, H3K27me3 has the same dynamic pattern with DNA methylation, while H3K-4me3 has a constant increased pattern. Furthermore, H3K27me3 has similar variation trends with DNA methylation both across these DC-dominant DMRs and in the DMR-associated gene loci.
Objective: Combinatorial epigenetic regulationis critical to maintain unique cell identity. However, dynamic epigenomes of human primary cells, particularly immune cell lineages undergoing differentiation, have rarely been reported. Methods: Using human primary lineage of monocytes, monocyte-derived immature dendritic cells(im DCs) and LPSmatured DCs(m DCs) representing the dynamic process of antigen-presenting cell differentiation and maturation, we established base-resolution DNA methylomes, H3K4me3 and H3K27me3 maps and mRNA transcriptome of this dynamic lineage.Results: Comparative analysis of dynamic epigenomic patterns revealed that DNA demethylation during monocytes differentiation into im DCs and de novo methylation during DCs maturation are DC-dominant DNA methylation variations. These genome-wide differential DNA methylation regions(DMRs) are mostly outside Cp G islands. Conclusion: For histone modifications, H3K27me3 has the same dynamic pattern with DNA methylation, while H3K-4me3 has a constant increased pattern. Furthermore, H3K27me3 has similar variation trends with DNA methylation both across these DC-dominant DMRs and in the DMR-associated gene loci.
Objective: Combinatorial epigenetic regulationis critical to maintain unique cell identity. However, dynamic epigenomes of human primary cells, particularly immune cell lineages undergoing differentiation, have rarely been reported. Methods: Using human primary lineage of monocytes, monocyte-derived immature dendritic cells(im DCs) and LPSmatured DCs(m DCs) representing the dynamic process of antigen-presenting cell differentiation and maturation, we established base-resolution DNA methylomes, H3K4me3 and H3K27me3 maps and mRNA transcriptome of this dynamic lineage.Results: Comparative analysis of dynamic epigenomic patterns revealed that DNA demethylation during monocytes differentiation into im DCs and de novo methylation during DCs maturation are DC-dominant DNA methylation variations. These genome-wide differential DNA methylation regions(DMRs) are mostly outside Cp G islands. Conclusion: For histone modifications, H3K27me3 has the same dynamic pattern with DNA methylation, while H3K-4me3 has a constant increased pattern. Furthermore, H3K27me3 has similar variation trends with DNA methylation both across these DC-dominant DMRs and in the DMR-associated gene loci.
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