摘要
本实验用浓度为0.15 mg/mL的孔雀石绿药浴花鲈1 h,研究孔雀石绿及其代谢产物隐色孔雀石绿在花鲈各组织中的分布与消除规律。采用超高效液相色谱串联质谱法快速检测孔雀石绿、隐色孔雀石绿在花鲈的背肌、鱼皮、肝脏的含量。结果表明,在消除期间,LMG的浓度始终高于MG的。MG、LMG在肝脏中的浓度最高。孔雀石绿在花鲈3种组织中的浓度由高到低的顺序为:肝脏(94.86±8.12μg/kg)>鱼皮(19.35±1.88μg/kg)>背肌(7.47±0.45μg/kg)。MG在背肌、鱼皮、肝脏中分别于168 h、960 h、2160 h时检测不到。隐色孔雀石绿在花鲈3种组织中的浓度由高到低的顺序为:肝脏(3607.13±189.56μg/kg)>背肌(103.65±14.97μg/kg)>鱼皮(82.10±7.64μg/kg)。消除2544 h后,背肌、鱼皮、肝脏中的LMG含量仍然高于检测限。
The distribution and depletion rules of MG and its metabolite LMG in organizations of perch were investigated, that were dipped in the concentration of 0.15 mg/mL MG for 1h. Skin,back muscle and liver of perch were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry carrying out qualitative. The concentration of LMG was much higher than that of MG during the experiment. The concentration of MG and LMG in liver was highest. The result of MG concentrations in back muscle, skin, liver of perch showed that the order of 3 kinds of tissues concentration levels of MG from high to low were respectively liver(94.86±8.12 μg/kg), skin(19.35±1.88 μg/kg), back muscle(7.47±0.45 μg/kg). MG was not detected in back muscle, skin, liver of perch at 168 h 、 960 h 、 2160 h. The order of 3 kinds of tissues concentration levels of LMG from high to low were respectively liver(3607.13±189.56μg/kg), back muscle(103.65±14.97 μg/kg), skin(82.10±7.64 μg/kg). Even after 2544 h of elimination, LMG in back muscle, skin, liver can be detected. Some reference is provided for the quality and safety risk assessment of aquatic products.
引文