电纺纤维的刚度对iPS-MSCs的黏附和迁移影响
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- 英文论文题名：Effects of Electrospun Fiber Stiffness on Adhesion and Migration of iPS-MSCs
- 论文作者：余哲泡 ; 袁卉华 ; 易兵成 ; 王先流 ; 张彦中
- 英文论文作者：Zhepao Yu ; Huihua Yuan ; Bingcheng Yi ; Xianliu Wang ; Yanzhong Zhang ; College of Chemistry ; Chemical Engineering & Biotechnology ; Donghua University
- 年：2016
- 作者机构：东华大学化学化工与生物工程学院;
- 论文关键词：电纺纤维 ; 纤维刚度 ; iPS-MSCs ; 细胞黏附 ; 细胞迁移
- 会议召开时间：2016-11-18
- 会议录名称：中国第四届静电纺丝大会（CICE 2016）摘要集
- 语种：中文
- 分类号：TQ340.6;R318.08
- 学会代码：ZGNM
- 会议名称：中国第四届静电纺丝大会(CICE 2016)
- 会议地点：中国北京
- 主办单位：中国复合材料学会超细纤维材料分会（筹）
- 学会名称：中关村科创纳米技术创新研究会
- 页数：1
- 文件大小：544k
- 原文格式：D
- 会议级别：全国
摘要
诱导多能干细胞来源的间充质干细胞(i PS-MSCs)是近年来一类广受关注的组织工程用细胞来源[1]。利用生物材料支架的理化特性调控细胞行为是当前的一个研究热点。其中,电纺纤维的刚度对细胞功能具有重要影响[2],但针对电纺纤维刚度调控i PS-MSCs行为尚未见报道。本研究采用退火处理方法调控电纺聚左旋乳酸(PLLA)纤维的刚度,并评价纤维刚度变化对i PS-MSCs的形貌、增殖和迁移行为的影响。结果表明:退火处理对PLLA纤维的直径影响不大(退火前1.26±0.25μm,退火后1.24±0.26μm),但可显著增加纤维结晶度和力学性能,导致纤维刚度增加1.73倍;将i PS-MSCs在经退火处理的PLLA纤维支架上培养24 h后的细胞骨架铺展面积及7 d后的细胞增殖,分别是未处理的1.78倍和1.18倍。迁移实验表明,纤维刚度的提高有利于i PS-MSCs的迁移并促进迁移相关基因Integrinβ1、Rho A和Rock1的上调。这些结果表明,通过退火处理提高电纺PLLA纤维刚度对i PS-MSCs的黏附、增殖和迁移有着促进作用,可作为组织工程中调节细胞生物功能的一个重要手段。
Recently, induced pluripotent stem cells derived mesenchymal stem cells(i PS-MSCs) have emerged as a kind of well-liked cell source in the field of tissue engineering. Varying the physicochemical properties of biomaterial scaffolds is a widely employed strategy to regulate cell behaviors. Stiffness of electrospun fibers has been identified to play an important role in cell functions. However, the influence of fiber stiffness on i PS-MSCs has not been explored yet. In this study, i PS-MSCs were cultured on as-electrospun and annealed electrospun PLLA fibers to investigate the potential impact of fiber stiffness on the i PS-MSCs' functioning. The results showed that the annealing treatment, which had less effect on the diameter of PLLA fibers, could increase fiber stiffness and promote i PS-MSCs adhesion, proliferation and migration. This study demonstrated that increasing fiber stiffness can be used as an effective means to promote functional expression of cells in tissue engineering.
Recently, induced pluripotent stem cells derived mesenchymal stem cells(i PS-MSCs) have emerged as a kind of well-liked cell source in the field of tissue engineering. Varying the physicochemical properties of biomaterial scaffolds is a widely employed strategy to regulate cell behaviors. Stiffness of electrospun fibers has been identified to play an important role in cell functions. However, the influence of fiber stiffness on i PS-MSCs has not been explored yet. In this study, i PS-MSCs were cultured on as-electrospun and annealed electrospun PLLA fibers to investigate the potential impact of fiber stiffness on the i PS-MSCs' functioning. The results showed that the annealing treatment, which had less effect on the diameter of PLLA fibers, could increase fiber stiffness and promote i PS-MSCs adhesion, proliferation and migration. This study demonstrated that increasing fiber stiffness can be used as an effective means to promote functional expression of cells in tissue engineering.
引文
[1]Xie,J.,et al.,Acta Biomaterialia,2016,29,365-379
[2]Yuan,H.H.,et al.,Acta Biomaterialia,2016,42,247-257
[2]Yuan,H.H.,et al.,Acta Biomaterialia,2016,42,247-257