Mage-d4 is a suitable target for poor prognosis and potential immunotherapy involved in glioma tumorigenesis
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- 英文论文题名：Mage-d4 is a suitable target for poor prognosis and potential immunotherapy involved in glioma tumorigenesis
- 论文作者：Zhang Qing-Mei ; Luo Bin ; Ge Yingying ; Chen Fang ; Fu Jun ; Peng Yang ; Liu Chang ; Luo Guo-Rong ; Xiao Shao-Wen ; Xie Xiao-Xun
- 英文论文作者：Zhang Qing-Mei ; Luo Bin ; Ge Yingying ; Chen Fang ; Fu Jun ; Peng Yang ; Liu Chang ; Luo Guo-Rong ; Xiao Shao-Wen ; Xie Xiao-Xun ; Department of Histology and Embryology ; School of Pre-clinical Medicine ; Guangxi medical university ; Department of Neurosurgery ; First Affiliated Hospital ; Guangxi medical university
- 年：2016
- 作者机构：Department of Histology and Embryology,School of Pre-clinical Medicine,Guangxi medical university;Department of Neurosurgery,First Affiliated Hospital,Guangxi medical university;
- 英文论文关键词：Mage-d4 ; glioma ; tumorigenesis ; prognosis ; immunotherapy
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会壁报交流集
- 英文会议录名称：Proceedings of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R739.41
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：965k
- 原文格式：D
- 会议级别：全国
摘要
Background:Melanoma-associated antigen(Mage)-d4 was originally identified as a glioma-specific antigen.This study aimed to investigate the expression of Mage-d4 and its roles in tumorigenesis and cellular immune response to determine its potential as a prognosis and immunotherapeutic target for glioma.Methods:Mage-d4 expression was evaluated by immunohistochemistry(IHC) in 124 glioma specimens,among which were 84 patients followed up.To investigate the biological role of Mage-d4 in tumorigenesis,its expression was up-or down-regulated in glioma cell lines A172 and U251,followed by ts phenotypic effects were evaluated in vitro.For cellular immune response,human monocyte-derived dendritic cells(DCs) loaded with Mage-d4 recombinant protein,was used to stimulate in vitro to generate Maged4-specific cytotoxic T lymphocytes(CTLs) as demonstrated by IFN-γ production and cytotoxicity against glioma cells.Results:Mage-d4 protein was observed in 97 out of 124(78.2%) of glioma tissues tested.Furthermore,it was found to positively correlate with WHO grade and to be an independent prognostic factor for poor outcome.Down-regulation of Mage-d4 expression in U251 significantly suppressed cell growth,migration and invasion.On the contrary,these malignant behaviors were enhanced by up-regulation of Mage-d4 expression in A172.IFN-γ-producing Maged4-specific CTLs lysed Maged4-expressing HLA-A2+ U251 instead of Maged4-expressing HLA-A2-A172 in dose-dependent and HLA-A2-restricted manners.Conclusion:Our findings suggest that Mage-d4 overexpression contributes to the tumorigenesis of glioma cells and may serve as a potentially prognostic biomarker and an attractive target of immunotherapy in glioma.
Background:Melanoma-associated antigen(Mage)-d4 was originally identified as a glioma-specific antigen.This study aimed to investigate the expression of Mage-d4 and its roles in tumorigenesis and cellular immune response to determine its potential as a prognosis and immunotherapeutic target for glioma.Methods:Mage-d4 expression was evaluated by immunohistochemistry(IHC) in 124 glioma specimens,among which were 84 patients followed up.To investigate the biological role of Mage-d4 in tumorigenesis,its expression was up-or down-regulated in glioma cell lines A172 and U251,followed by ts phenotypic effects were evaluated in vitro.For cellular immune response,human monocyte-derived dendritic cells(DCs) loaded with Mage-d4 recombinant protein,was used to stimulate in vitro to generate Maged4-specific cytotoxic T lymphocytes(CTLs) as demonstrated by IFN-γ production and cytotoxicity against glioma cells.Results:Mage-d4 protein was observed in 97 out of 124(78.2%) of glioma tissues tested.Furthermore,it was found to positively correlate with WHO grade and to be an independent prognostic factor for poor outcome.Down-regulation of Mage-d4 expression in U251 significantly suppressed cell growth,migration and invasion.On the contrary,these malignant behaviors were enhanced by up-regulation of Mage-d4 expression in A172.IFN-γ-producing Maged4-specific CTLs lysed Maged4-expressing HLA-A2+ U251 instead of Maged4-expressing HLA-A2-A172 in dose-dependent and HLA-A2-restricted manners.Conclusion:Our findings suggest that Mage-d4 overexpression contributes to the tumorigenesis of glioma cells and may serve as a potentially prognostic biomarker and an attractive target of immunotherapy in glioma.
Background:Melanoma-associated antigen(Mage)-d4 was originally identified as a glioma-specific antigen.This study aimed to investigate the expression of Mage-d4 and its roles in tumorigenesis and cellular immune response to determine its potential as a prognosis and immunotherapeutic target for glioma.Methods:Mage-d4 expression was evaluated by immunohistochemistry(IHC) in 124 glioma specimens,among which were 84 patients followed up.To investigate the biological role of Mage-d4 in tumorigenesis,its expression was up-or down-regulated in glioma cell lines A172 and U251,followed by ts phenotypic effects were evaluated in vitro.For cellular immune response,human monocyte-derived dendritic cells(DCs) loaded with Mage-d4 recombinant protein,was used to stimulate in vitro to generate Maged4-specific cytotoxic T lymphocytes(CTLs) as demonstrated by IFN-γ production and cytotoxicity against glioma cells.Results:Mage-d4 protein was observed in 97 out of 124(78.2%) of glioma tissues tested.Furthermore,it was found to positively correlate with WHO grade and to be an independent prognostic factor for poor outcome.Down-regulation of Mage-d4 expression in U251 significantly suppressed cell growth,migration and invasion.On the contrary,these malignant behaviors were enhanced by up-regulation of Mage-d4 expression in A172.IFN-γ-producing Maged4-specific CTLs lysed Maged4-expressing HLA-A2+ U251 instead of Maged4-expressing HLA-A2-A172 in dose-dependent and HLA-A2-restricted manners.Conclusion:Our findings suggest that Mage-d4 overexpression contributes to the tumorigenesis of glioma cells and may serve as a potentially prognostic biomarker and an attractive target of immunotherapy in glioma.
引文