Mitogen-activated protein kinase cascade MKK7-MPK6 plays important roles in plant development and regulates shoot branching by phosphorylating PIN1 in Arabidopsis
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- 英文论文题名：Mitogen-activated protein kinase cascade MKK7-MPK6 plays important roles in plant development and regulates shoot branching by phosphorylating PIN1 in Arabidopsis
- 论文作者：Weiyan Jia ; Baohua Li ; Shujia Li ; Yan Liang ; Xiaowei Wu ; Mei Ma ; Jiyao Wang ; Yueyue Cai ; Yuanya Zhang ; Yingchun Wang ; Jiayang Li ; Yonghong Wang
- 英文论文作者：Weiyan Jia ; Baohua Li ; Shujia Li ; Yan Liang ; Xiaowei Wu ; Mei Ma ; Jiyao Wang ; Yueyue Cai ; Yuanya Zhang ; Yingchun Wang ; Jiayang Li ; Yonghong Wang ; State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing) ; CAS Center for Excellence in Molecular Plant Sciences ; Institute of Genetics and Developmental Biology ; Chinese Academy of Sciences ; Department of Human Oncology ; University of Wisconsin ; School of Medicine and Public Health ; Department of Plant Sciences ; University of California ; State Key Laboratory of Molecular Developmental Biology ; Institute of Genetics and Developmental Biology ; Chinese Academy of Sciences
- 年：2016
- 作者机构：State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing),CAS Center for Excellence in Molecular Plant Sciences,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences;Department of Human Oncology,University of Wisconsin,School of Medicine and Public Health;Department of Plant Sciences,University of California;State Key Laboratory of Molecular Developmental Biology,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences;
- 英文论文关键词：MKK7 ; MPK6 ; phosphorylation ; PIN1 ; shoot branching
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q945
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：848k
- 原文格式：D
- 会议级别：全国
摘要
Emerging evidences exhibit that mitogen-activated protein kinase(MAPK) signaling pathways are connected with many aspects of plant development. The complexity of MAPK cascades raises challenges not only to identify the MAPK module in planta but also to define the specific role of an individual module. Our previous study have characterized an Arabidopsis bushy and dwarf1(bud1) mutant,in which the MKK7 was constitutively activated,resulting in multiple phenotypic alterations and activated the mobile signal of systemic acquired resistance. To elucidate the specificity of MKK7 and its downstream MAPKs in multiple biological processes,we systemically screened the downstream substrates of MKK7 in vitro. We found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta. Genetic analysis showed that MKK7-MPK6 cascade is specifically responsible for the regulation of shoot branching,hypocotyl gravitropism,filament elongation,and lateral root formation. We further demonstrated that the MKK7-MPK6 cascade controls shoot branching by phosphorylating Ser 337 on PIN1,which affects the basal localization of PIN1 in xylem parenchyma cells and polar auxin transport in the primary stem. Our findings specify the functions of the MKK7-MPK6 cascade,and explain how MKK7-MPK6 signaling pathway regulates polar auxin transport(PAT) through the specific substrate PIN1 to determine shoot branching in Arabidopsis,establishing a molecular link between the MAPK cascade and auxin-regulated plant development.
Emerging evidences exhibit that mitogen-activated protein kinase(MAPK) signaling pathways are connected with many aspects of plant development. The complexity of MAPK cascades raises challenges not only to identify the MAPK module in planta but also to define the specific role of an individual module. Our previous study have characterized an Arabidopsis bushy and dwarf1(bud1) mutant,in which the MKK7 was constitutively activated,resulting in multiple phenotypic alterations and activated the mobile signal of systemic acquired resistance. To elucidate the specificity of MKK7 and its downstream MAPKs in multiple biological processes,we systemically screened the downstream substrates of MKK7 in vitro. We found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta. Genetic analysis showed that MKK7-MPK6 cascade is specifically responsible for the regulation of shoot branching,hypocotyl gravitropism,filament elongation,and lateral root formation. We further demonstrated that the MKK7-MPK6 cascade controls shoot branching by phosphorylating Ser 337 on PIN1,which affects the basal localization of PIN1 in xylem parenchyma cells and polar auxin transport in the primary stem. Our findings specify the functions of the MKK7-MPK6 cascade,and explain how MKK7-MPK6 signaling pathway regulates polar auxin transport(PAT) through the specific substrate PIN1 to determine shoot branching in Arabidopsis,establishing a molecular link between the MAPK cascade and auxin-regulated plant development.
Emerging evidences exhibit that mitogen-activated protein kinase(MAPK) signaling pathways are connected with many aspects of plant development. The complexity of MAPK cascades raises challenges not only to identify the MAPK module in planta but also to define the specific role of an individual module. Our previous study have characterized an Arabidopsis bushy and dwarf1(bud1) mutant,in which the MKK7 was constitutively activated,resulting in multiple phenotypic alterations and activated the mobile signal of systemic acquired resistance. To elucidate the specificity of MKK7 and its downstream MAPKs in multiple biological processes,we systemically screened the downstream substrates of MKK7 in vitro. We found that MPK3 and MPK6 are the substrates for phosphorylation by MKK7 in planta. Genetic analysis showed that MKK7-MPK6 cascade is specifically responsible for the regulation of shoot branching,hypocotyl gravitropism,filament elongation,and lateral root formation. We further demonstrated that the MKK7-MPK6 cascade controls shoot branching by phosphorylating Ser 337 on PIN1,which affects the basal localization of PIN1 in xylem parenchyma cells and polar auxin transport in the primary stem. Our findings specify the functions of the MKK7-MPK6 cascade,and explain how MKK7-MPK6 signaling pathway regulates polar auxin transport(PAT) through the specific substrate PIN1 to determine shoot branching in Arabidopsis,establishing a molecular link between the MAPK cascade and auxin-regulated plant development.
引文