A Promoter-less Transposon for the Trapping of rplY,a Novel Gene Crucial to Virulence in Pectobacterium carotovorum subsp.carotovorum that is Activated by Leaf Extracts from Zantedeschia elliotiana
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- 英文论文题名：A Promoter-less Transposon for the Trapping of rplY,a Novel Gene Crucial to Virulence in Pectobacterium carotovorum subsp.carotovorum that is Activated by Leaf Extracts from Zantedeschia elliotiana
- 论文作者：JIANG Huan ; JIANG Meng-yi ; YANG Liu-ke ; MA Lin ; WANG Chun-ting ; WANG Huan ; QIAN Gou-liang ; FAN Jia-qin
- 英文论文作者：JIANG Huan ; JIANG Meng-yi ; YANG Liu-ke ; MA Lin ; WANG Chun-ting ; WANG Huan ; QIAN Gou-liang ; FAN Jia-qin ; Department of Plant Pathology ; Nanjing Agricultural University ; Center for Biotechnology ; Chongqing Yudongnan Academy of Agricultural Sciences
- 年：2016
- 作者机构：Department of Plant Pathology,Nanjing Agricultural University;Center for Biotechnology,Chongqing Yudongnan Academy of Agricultural Sciences;
- 英文论文关键词：Bacterial-plant interaction ; Pectobacterium carotovorum ; Mariner transposon ; Plant extract ; Zantedeschia elliotiana
- 会议召开时间：2016-08-05
- 会议录名称：中国植物病理学会2016年学术年会论文集
- 英文会议录名称：Proceedings of the Annual Meeting of Chinese Society for Plant Pathology(2016)
- 语种：英文
- 分类号：S432.4
- 学会代码：ZGVS
- 会议名称：中国植物病理学会2016年学术年会
- 会议地点：中国江苏南京
- 主办单位：中国植物病理学会
- 学会名称：中国植物病理学会
- 页数：1
- 文件大小：60k
- 原文格式：O
- 会议级别：全国
摘要
Previous studies have demonstrated the interactions between bacterial pathogens and plant hosts,from the viewpoint both of the plant and bacterium,using many approaches,including proteomic and transcriptomic analyses and genomic and post-genomic studies.However,there are some limitations due to some undetectable proteins using the current tools or incomplete understanding due to miscellaneous post-transcriptional processes.In this study,we constructed a library of approximately 6 000 insertion mutants of the Pectobacterium carotovorum subsp.carotovorum strain PccS1 using mariner,a promoter-less kanamycin resistance transposon,from which approximately 500 insertional mutants were found to have kanamycin resistance dependent on plant extracts from the leaves of Zantedeschia elliotiana;only one of these mutants showed seriously attenuated virulence on the hosts and impaired multiplication in the media without the plant extract.The gene in the insertion site of the mutantwas namedrplY,and it was activated in PccS1 both at the gene transcript and protein expression levels with a significant increase in the promoter strength when the cells encountered the plant extract.It was revealed that deletion of rplY reduced the virulence of the pathogen and also decreased the motility and production of extracellular enzymes,such as protease,pectatelyase and cellulase,but did not affect the formation of biofilm,compared with the wild-type strain.The strain of the gene deletion mutant complementary withrplY partly restored the virulence,motility and production of the exo-enzymes.Meanwhile,the virulence of the rplY gene deletion mutant could be partly restored by the plant extract.The data indicate that the plant extract induces PccS1 virulence and multiplication partly throughrplY activation,andrplY is crucial to virulence,motility and exo-enzyme production in P.carotovorum.This work is a valuable complement to the approaches on bacterial-plant interactions and on the identification of novel genes associated with pathogenicity.
Previous studies have demonstrated the interactions between bacterial pathogens and plant hosts,from the viewpoint both of the plant and bacterium,using many approaches,including proteomic and transcriptomic analyses and genomic and post-genomic studies.However,there are some limitations due to some undetectable proteins using the current tools or incomplete understanding due to miscellaneous post-transcriptional processes.In this study,we constructed a library of approximately 6 000 insertion mutants of the Pectobacterium carotovorum subsp.carotovorum strain PccS1 using mariner,a promoter-less kanamycin resistance transposon,from which approximately 500 insertional mutants were found to have kanamycin resistance dependent on plant extracts from the leaves of Zantedeschia elliotiana;only one of these mutants showed seriously attenuated virulence on the hosts and impaired multiplication in the media without the plant extract.The gene in the insertion site of the mutantwas namedrplY,and it was activated in PccS1 both at the gene transcript and protein expression levels with a significant increase in the promoter strength when the cells encountered the plant extract.It was revealed that deletion of rplY reduced the virulence of the pathogen and also decreased the motility and production of extracellular enzymes,such as protease,pectatelyase and cellulase,but did not affect the formation of biofilm,compared with the wild-type strain.The strain of the gene deletion mutant complementary withrplY partly restored the virulence,motility and production of the exo-enzymes.Meanwhile,the virulence of the rplY gene deletion mutant could be partly restored by the plant extract.The data indicate that the plant extract induces PccS1 virulence and multiplication partly throughrplY activation,andrplY is crucial to virulence,motility and exo-enzyme production in P.carotovorum.This work is a valuable complement to the approaches on bacterial-plant interactions and on the identification of novel genes associated with pathogenicity.
Previous studies have demonstrated the interactions between bacterial pathogens and plant hosts,from the viewpoint both of the plant and bacterium,using many approaches,including proteomic and transcriptomic analyses and genomic and post-genomic studies.However,there are some limitations due to some undetectable proteins using the current tools or incomplete understanding due to miscellaneous post-transcriptional processes.In this study,we constructed a library of approximately 6 000 insertion mutants of the Pectobacterium carotovorum subsp.carotovorum strain PccS1 using mariner,a promoter-less kanamycin resistance transposon,from which approximately 500 insertional mutants were found to have kanamycin resistance dependent on plant extracts from the leaves of Zantedeschia elliotiana;only one of these mutants showed seriously attenuated virulence on the hosts and impaired multiplication in the media without the plant extract.The gene in the insertion site of the mutantwas namedrplY,and it was activated in PccS1 both at the gene transcript and protein expression levels with a significant increase in the promoter strength when the cells encountered the plant extract.It was revealed that deletion of rplY reduced the virulence of the pathogen and also decreased the motility and production of extracellular enzymes,such as protease,pectatelyase and cellulase,but did not affect the formation of biofilm,compared with the wild-type strain.The strain of the gene deletion mutant complementary withrplY partly restored the virulence,motility and production of the exo-enzymes.Meanwhile,the virulence of the rplY gene deletion mutant could be partly restored by the plant extract.The data indicate that the plant extract induces PccS1 virulence and multiplication partly throughrplY activation,andrplY is crucial to virulence,motility and exo-enzyme production in P.carotovorum.This work is a valuable complement to the approaches on bacterial-plant interactions and on the identification of novel genes associated with pathogenicity.
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