Integrated epigenetic maps of cotton fiber provide novel insights into staged single-cell differentiation
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- 英文论文题名：Integrated epigenetic maps of cotton fiber provide novel insights into staged single-cell differentiation
- 论文作者：Maojun Wang ; Pengcheng Wang ; Lili Tu ; Sitao Zhu ; Lin Zhang ; Zhonghua Li ; Qinghua Zhang ; Daojun Yuan ; Xianlong Zhang
- 英文论文作者：Maojun Wang ; Pengcheng Wang ; Lili Tu ; Sitao Zhu ; Lin Zhang ; Zhonghua Li ; Qinghua Zhang ; Daojun Yuan ; Xianlong Zhang ; National Key Laboratory of Crop Genetic Improvement ; Huazhong Agricultural University
- 年：2016
- 作者机构：National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University;
- 英文论文关键词：DNA methylation ; cotton fiber ; single-cell
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：S562;Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：842k
- 原文格式：D
- 会议级别：全国
摘要
DNA methylation is highlighted for its great importance in regulating plant development,but its function associated with single-cell differentiation remains undetermined. Here,we used cotton fiber,specifically,the epidermal hairs on the cotton ovule,as a model to investigate the regulatory role of DNA methylation in cell differentiation. Global disruption of DNA methylation was first demonstrated to re-activate a large number of genomic loci and depress cotton fiber development. We constructed single-base resolution maps of DNA methylation dynamics representing each fiber developmental stage. The CHH(H=A,T,or C) DNA methylation level was increased during fiber development,accompanied by a decreased pattern of RNA-directed DNA methylation(Rd DM). Examination of nucleosome positioning revealed a gradual eu-to hetero-chromatin transition for chromatin reprogramming in developing fibers,coupled with increased DNA methylation. Compared with cotton ovules,increased DNA methylation in fibers was demonstrated to be predominantly mediated by an active H3K9me2-dependent pathway instead of the inactive Rd DM pathway,especially in heterochromatic regions. We examined how asymmetric DNA methylation contributed to homoeologous gene expression bias,illustrating a sub-genomic collaborated regulation of allotetraploid cotton fiber development. Furthermore,integrated multi-omics analyses revealed that dynamic DNA methylation could play a role in the regulation of lipid biosynthesis and spatio-temporal modulation of reactive oxygen species for staged fiber differentiation. Our study established a framework for understanding the role of DNA methylation and chromatin reprogramming during fiber development and also provided novel insights into the epigenetic regulation of single-cell differentiation in plants.
DNA methylation is highlighted for its great importance in regulating plant development,but its function associated with single-cell differentiation remains undetermined. Here,we used cotton fiber,specifically,the epidermal hairs on the cotton ovule,as a model to investigate the regulatory role of DNA methylation in cell differentiation. Global disruption of DNA methylation was first demonstrated to re-activate a large number of genomic loci and depress cotton fiber development. We constructed single-base resolution maps of DNA methylation dynamics representing each fiber developmental stage. The CHH(H=A,T,or C) DNA methylation level was increased during fiber development,accompanied by a decreased pattern of RNA-directed DNA methylation(Rd DM). Examination of nucleosome positioning revealed a gradual eu-to hetero-chromatin transition for chromatin reprogramming in developing fibers,coupled with increased DNA methylation. Compared with cotton ovules,increased DNA methylation in fibers was demonstrated to be predominantly mediated by an active H3K9me2-dependent pathway instead of the inactive Rd DM pathway,especially in heterochromatic regions. We examined how asymmetric DNA methylation contributed to homoeologous gene expression bias,illustrating a sub-genomic collaborated regulation of allotetraploid cotton fiber development. Furthermore,integrated multi-omics analyses revealed that dynamic DNA methylation could play a role in the regulation of lipid biosynthesis and spatio-temporal modulation of reactive oxygen species for staged fiber differentiation. Our study established a framework for understanding the role of DNA methylation and chromatin reprogramming during fiber development and also provided novel insights into the epigenetic regulation of single-cell differentiation in plants.
DNA methylation is highlighted for its great importance in regulating plant development,but its function associated with single-cell differentiation remains undetermined. Here,we used cotton fiber,specifically,the epidermal hairs on the cotton ovule,as a model to investigate the regulatory role of DNA methylation in cell differentiation. Global disruption of DNA methylation was first demonstrated to re-activate a large number of genomic loci and depress cotton fiber development. We constructed single-base resolution maps of DNA methylation dynamics representing each fiber developmental stage. The CHH(H=A,T,or C) DNA methylation level was increased during fiber development,accompanied by a decreased pattern of RNA-directed DNA methylation(Rd DM). Examination of nucleosome positioning revealed a gradual eu-to hetero-chromatin transition for chromatin reprogramming in developing fibers,coupled with increased DNA methylation. Compared with cotton ovules,increased DNA methylation in fibers was demonstrated to be predominantly mediated by an active H3K9me2-dependent pathway instead of the inactive Rd DM pathway,especially in heterochromatic regions. We examined how asymmetric DNA methylation contributed to homoeologous gene expression bias,illustrating a sub-genomic collaborated regulation of allotetraploid cotton fiber development. Furthermore,integrated multi-omics analyses revealed that dynamic DNA methylation could play a role in the regulation of lipid biosynthesis and spatio-temporal modulation of reactive oxygen species for staged fiber differentiation. Our study established a framework for understanding the role of DNA methylation and chromatin reprogramming during fiber development and also provided novel insights into the epigenetic regulation of single-cell differentiation in plants.
引文