Repressed function of the C terminal domain of GS3 in grain size regulation
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- 英文论文题名：Repressed function of the C terminal domain of GS3 in grain size regulation
- 论文作者：Shengyuan Sun ; Yidan Ouyang ; Qifa Zhang
- 英文论文作者：Shengyuan Sun ; Yidan Ouyang ; Qifa Zhang ; National Key Laboratory of Crop Genetic Improvement ; Huazhong Agricultural University
- 年：2016
- 作者机构：National Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University;
- 英文论文关键词：grain size ; GS3 gene ; OSR ; cysteine-rich domain ; protein level
- 会议召开时间：2016-10-09
- 会议录名称：2016年全国植物生物学大会摘要集
- 语种：英文
- 分类号：Q943.2
- 学会代码：ZGZO
- 会议名称：2016年全国植物生物学大会
- 会议地点：中国湖北武汉
- 主办单位：中国遗传学会、中国细胞生物学学会、中国植物学会、中国植物生理与植物分子生物学学会、中国作物学会
- 学会名称：中国植物学会
- 页数：1
- 文件大小：851k
- 原文格式：D
- 会议级别：全国
摘要
Grain yield in many cereal crops is largely determined by grain size. Our group previously established that GS3-4 allele showing stronger effect on reducing grain size due to its deficiency of the C terminal cysteine-rich domain,compared with the GS3-1 allele. Here show that the cysteine-rich domain cloud not interact with the N terminal(OSR) domain,excluding the possibility of head–to-tail interaction to block the function of OSR. We detected the protein level of the GS3-1 and GS3-4 transgenic plant which under ubiquitin promoter and fused with Flag tag in the C terminal. As expect,the enhanced phenotype mediated by overexpressing GS3-4 was correlated with the increased protein level but not m RNA level. Accumulation of GS3-1 protein but not GS3-4 protein was detected compared with the untreated control when treated with the proteasome inhibitor MG-132,suggesting that GS3-1 might degrade via C terminal domain through the proteasome-mediated pathway. These findings added to the understanding of the molecular mechanism with respect to GS3 in grain size regulation.
Grain yield in many cereal crops is largely determined by grain size. Our group previously established that GS3-4 allele showing stronger effect on reducing grain size due to its deficiency of the C terminal cysteine-rich domain,compared with the GS3-1 allele. Here show that the cysteine-rich domain cloud not interact with the N terminal(OSR) domain,excluding the possibility of head–to-tail interaction to block the function of OSR. We detected the protein level of the GS3-1 and GS3-4 transgenic plant which under ubiquitin promoter and fused with Flag tag in the C terminal. As expect,the enhanced phenotype mediated by overexpressing GS3-4 was correlated with the increased protein level but not m RNA level. Accumulation of GS3-1 protein but not GS3-4 protein was detected compared with the untreated control when treated with the proteasome inhibitor MG-132,suggesting that GS3-1 might degrade via C terminal domain through the proteasome-mediated pathway. These findings added to the understanding of the molecular mechanism with respect to GS3 in grain size regulation.
Grain yield in many cereal crops is largely determined by grain size. Our group previously established that GS3-4 allele showing stronger effect on reducing grain size due to its deficiency of the C terminal cysteine-rich domain,compared with the GS3-1 allele. Here show that the cysteine-rich domain cloud not interact with the N terminal(OSR) domain,excluding the possibility of head–to-tail interaction to block the function of OSR. We detected the protein level of the GS3-1 and GS3-4 transgenic plant which under ubiquitin promoter and fused with Flag tag in the C terminal. As expect,the enhanced phenotype mediated by overexpressing GS3-4 was correlated with the increased protein level but not m RNA level. Accumulation of GS3-1 protein but not GS3-4 protein was detected compared with the untreated control when treated with the proteasome inhibitor MG-132,suggesting that GS3-1 might degrade via C terminal domain through the proteasome-mediated pathway. These findings added to the understanding of the molecular mechanism with respect to GS3 in grain size regulation.
引文