射频对膝关节软骨损伤模型中软骨细胞活性影响的实验研究
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- 英文论文题名：Experimental study on the effect of radiofrequency on chondrocyte activity in knee joint cartilage injury model
- 论文作者：罗景胜
- 英文论文作者：Luo Jingsheng
- 年：2017
- 作者机构：赣南医学院第一附属医院骨科;
- 论文关键词：射频消融 ; 膝关节软骨损伤 ; 软骨细胞活性
- 英文论文关键词：Radiofrequency ablation ; knee articular cartilage injury
- 会议召开时间：2017-05-26
- 会议录名称：2017年《中国组织化学与细胞化学杂志》临床研讨会论文集
- 语种：中文
- 分类号：R684.3
- 学会代码：ZHXB
- 会议名称：2017年《中国组织化学与细胞化学杂志》临床研讨会
- 会议地点：中国湖北武汉
- 主办单位：《中国组织化学与细胞化学杂志》社有限公司
- 学会名称：《中国组织化学与细胞化学杂志》社有限公司
- 页数：2
- 文件大小：242k
- 原文格式：O
- 会议级别：全国
摘要
目的:探讨射频对膝关节软骨损伤模型中软骨细胞活性影响的实验研究。方法:以牛膝关节软骨退变模型为研究对象,模拟膝关节镜环境,射频消融气化仪分别设置15W、40W、70W能量下对牛膝关节退变软骨处理1min,同时设置对照组,共4组,每组6份标本,对软骨组织进行培养,观察HE染色、FDA/PI双荧光染色、GAG释出率情况。结果:对照组空泡率为18.69%、死亡率为17.89%,与15W时的28.35%、44.39%比较差异无统计学意义(P>0.05);40W时空泡率为56.25、70W时为66.27%,死亡率均为100%,与对照组比较差异均具有统计学意义(P<0.05);第1、2天对照组与能力15W时GAG释出率比较差异无统计学意义(P>0.05),能力为40W、70W时GAG释出率均明显低于对照组,差异显著(P<0.05),第3天时各组GAG释出率比较差异无统计学意义(P>0.05)。结论:射频消融能量的增加可明显增加软骨细胞的死亡率,加重软骨基质的损伤;射频消融能量的大小与关节软骨细胞、基质的损伤为正相关关系。
Objective:To investigate the effect of radiofrequency on the activity of chondrocytes in articular cartilage injury model.Methods:Bovine knee articular cartilage degeneration model as the research object,the simulation environment of knee arthroscopy,radiofrequency ablation,40 W 15W gasification system were set up,70 W energy of Achyranthes articular cartilage 1min treatment,while control group,a total of 4 groups,each group of 6 specimens of cartilage tissue were cultured by HE staining and FDA/PI double staining,fluorescence the release rate of GAG.Results:The control group of cavitation rate was 18.69%,the mortality rate was 17.89%,no statistical significance compared with 15 W 28.35%,44.39%difference(P>0.05);40W space-time foam rate was56.25,70 W was 66.27%,the mortality rate was 100%,compared with the control group were statistically significant(P<0.05);first,2 the control group and the ability of 15 W day when the release rate of GAG had no significant difference(P>0.05),40 W 70W,GAG capacity release rate was significantly lower than the control group,the difference was significant(P<0.05),the release rate of GAG was no statistically significant difference between the third day(P>0.05).Conclusions:The increase of radiofrequency ablation energy can significantly increase the mortality rate of chondrocytes and aggravate the damage of cartilage matrix.The energy of radiofrequency ablation is positively correlated with the injury of articular cartilage cells and matrix.
Objective:To investigate the effect of radiofrequency on the activity of chondrocytes in articular cartilage injury model.Methods:Bovine knee articular cartilage degeneration model as the research object,the simulation environment of knee arthroscopy,radiofrequency ablation,40 W 15W gasification system were set up,70 W energy of Achyranthes articular cartilage 1min treatment,while control group,a total of 4 groups,each group of 6 specimens of cartilage tissue were cultured by HE staining and FDA/PI double staining,fluorescence the release rate of GAG.Results:The control group of cavitation rate was 18.69%,the mortality rate was 17.89%,no statistical significance compared with 15 W 28.35%,44.39%difference(P>0.05);40W space-time foam rate was56.25,70 W was 66.27%,the mortality rate was 100%,compared with the control group were statistically significant(P<0.05);first,2 the control group and the ability of 15 W day when the release rate of GAG had no significant difference(P>0.05),40 W 70W,GAG capacity release rate was significantly lower than the control group,the difference was significant(P<0.05),the release rate of GAG was no statistically significant difference between the third day(P>0.05).Conclusions:The increase of radiofrequency ablation energy can significantly increase the mortality rate of chondrocytes and aggravate the damage of cartilage matrix.The energy of radiofrequency ablation is positively correlated with the injury of articular cartilage cells and matrix.
引文
[1]张凯,王大平,刘建全等.双极射频能量处理时间可影响软骨细胞活性和软骨表面轮廓[J].中国组织工程研究与临床康复,2011,15(41):7623-7626.
[2]陈琦,王大平,朱伟民等.射频能量处理软骨损伤的急性效应[J].中国组织工程研究与临床康复,2011,15(46):8561-8564.
[3]毕明俊,李琴.射频消融术对肝细胞肝癌患者特异性T淋巴细胞活性的影响[J].临床肝胆病杂志,2007,23(6):451-453.
[4]冯凯,马宽生,高峻等.射频消融治疗脾功能亢进症对机体细胞免疫功能的影响[J].中华普通外科杂志,2009,24(2):114-118.
[5]谢浩,张静,李定伟等.微波辐射损伤大鼠神经胶质细胞的初步研究[J].武汉理工大学学报,2014,36(12):37-41,77.
[6]彭伟.射频消融肝癌与热疗对细胞活性影响研究进展[J].医药前沿,2012,(20):23.[7]Guang-Li Wang,Xiu-Fang Xu,Ling Qiu et al.Dual Responsive Enzyme Mimicking Activity of AgX(X=CI,Br,I)Nanoparticles and Its Application for Cancer Cell Detection[J].ACS applied materials&interfaces,2014,6(9):6434-6442.
[8]Rath,Alexander G.,Rehberg,Markus,Janke,Robert et al.The influence of cell growth and enzyme activity changes on intracellular metabolite dynamics in AGE1.HN.AAT cells[J].Journal of Biotechnology,2014,178:43-53.
[9]Aichinger,Ernst,Villar,Corina B.R.,Di Mambro,Riccardo et al.The CHD3 Chromatin Remodeler PICKLE and Polycomb Group Proteins Antagonistically Regulate Meristem Activity in the Arabidopsis Root[J].The Plant Cell,2011,23(3):1047-1060.
[10]Xiaodi Hao,Zhengqing Cai,Kunming Fu et al.Distinguishing activity decay and cell death from bacterial decay for two types of methanogens[J].Water research;A journal of the international water association,2012,46(4):1251-1259.
[2]陈琦,王大平,朱伟民等.射频能量处理软骨损伤的急性效应[J].中国组织工程研究与临床康复,2011,15(46):8561-8564.
[3]毕明俊,李琴.射频消融术对肝细胞肝癌患者特异性T淋巴细胞活性的影响[J].临床肝胆病杂志,2007,23(6):451-453.
[4]冯凯,马宽生,高峻等.射频消融治疗脾功能亢进症对机体细胞免疫功能的影响[J].中华普通外科杂志,2009,24(2):114-118.
[5]谢浩,张静,李定伟等.微波辐射损伤大鼠神经胶质细胞的初步研究[J].武汉理工大学学报,2014,36(12):37-41,77.
[6]彭伟.射频消融肝癌与热疗对细胞活性影响研究进展[J].医药前沿,2012,(20):23.[7]Guang-Li Wang,Xiu-Fang Xu,Ling Qiu et al.Dual Responsive Enzyme Mimicking Activity of AgX(X=CI,Br,I)Nanoparticles and Its Application for Cancer Cell Detection[J].ACS applied materials&interfaces,2014,6(9):6434-6442.
[8]Rath,Alexander G.,Rehberg,Markus,Janke,Robert et al.The influence of cell growth and enzyme activity changes on intracellular metabolite dynamics in AGE1.HN.AAT cells[J].Journal of Biotechnology,2014,178:43-53.
[9]Aichinger,Ernst,Villar,Corina B.R.,Di Mambro,Riccardo et al.The CHD3 Chromatin Remodeler PICKLE and Polycomb Group Proteins Antagonistically Regulate Meristem Activity in the Arabidopsis Root[J].The Plant Cell,2011,23(3):1047-1060.
[10]Xiaodi Hao,Zhengqing Cai,Kunming Fu et al.Distinguishing activity decay and cell death from bacterial decay for two types of methanogens[J].Water research;A journal of the international water association,2012,46(4):1251-1259.