摘要
<正>Methods:Cell viability was determined using the CCK-8 assay.To investigate the apoptosis mechanism induced by ESC-3,the expression of apoptosis-related(Bax,Bcl-2,P53)and pathway-related(Wnt2,β-catenin,notch1,notch2,Hes1)proteins were measured by Western blot and quantified using the Image J software.To determine the anti-tumor effects of ESC-3,1×106 A2780cells was injected subcutaneously into the right flank of Balb/c nude mice to build the tumor xenograft
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