Proteome Analysis on Lethal Effect of l_2 in the Sex-linked Balanced Lethal Strains of Silkworm, Bombyx mori
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- 英文论文题名：Proteome Analysis on Lethal Effect of l_2 in the Sex-linked Balanced Lethal Strains of Silkworm, Bombyx mori
- 论文作者：Jine Chen ; Baolong Niu ; Yongqiang Wang ; Yan Liu ; Peigang Liu ; Zhiqi Meng ; Boxiong Zhong
- 英文论文作者：Jine Chen ; Baolong Niu ; Yongqiang Wang ; Yan Liu ; Peigang Liu ; Zhiqi Meng ; Boxiong Zhong
- 年：2016
- 英文论文关键词：embryo ; 2-DE ; MALDI-TOF/TOF ; sex-linked balanced lethal ; silkworm
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：499k
- 原文格式：D
- 会议级别：全国
摘要
The sex-linked balanced lethal(SLBL)strains of silkworm serve as an effective system for sex-control in silkworm. To gain comprehensive insight into the effect of one sex-linked balanced lethal gene l_2, comparative proteomic analysis was carried out between the survival embryos(W~(+l1)Z~(l1+l_2)) and lethal embryos(W~(+l1)Z~(+l1l_2))before the lethal stage. The lethal stage of l_2 was confirmed by observing the typical dead embryo morphology. The two genotype embryos before lethal stage were distinguished using polymorphic simple sequence repeats(SSR) markers closely linked to l_2 on the sex chromosome. Finally, 11 differentially expressed protein spots were successfully identified by MALDI-TOF/TOF mass spectrometry(MS). Among them, only 1 protein identified as heat shock protein 20.4(HSP20.4)was up-regulated in the lethal embryos, while the other 10 were down-regulated. The up-regulation of HSP20.4 suggests that there may be abnormal polypeptides produced in the lethal embryos. The gene ontology(GO)annotation indicated those down-regulated proteins are involved in important biological processes including embryo development, nucleoside metabolism, t RNA splicing, translation and protein folding. The biological pathway analysis showed that those down-regulated proteins are mainly involved in spindle assemblage and morphogenesis. Based on our results, we suggest that the l_2 may be the mutant expressing abnormal polypeptides. Its expression has a negative effect on mitosis and morphogenesis processes. The death of the embryos may be caused by the accumulation of abnormal polypeptides and the handicap of cell proliferation and morphogenesis.
The sex-linked balanced lethal(SLBL)strains of silkworm serve as an effective system for sex-control in silkworm. To gain comprehensive insight into the effect of one sex-linked balanced lethal gene l_2, comparative proteomic analysis was carried out between the survival embryos(W~(+l1)Z~(l1+l_2)) and lethal embryos(W~(+l1)Z~(+l1l_2))before the lethal stage. The lethal stage of l_2 was confirmed by observing the typical dead embryo morphology. The two genotype embryos before lethal stage were distinguished using polymorphic simple sequence repeats(SSR) markers closely linked to l_2 on the sex chromosome. Finally, 11 differentially expressed protein spots were successfully identified by MALDI-TOF/TOF mass spectrometry(MS). Among them, only 1 protein identified as heat shock protein 20.4(HSP20.4)was up-regulated in the lethal embryos, while the other 10 were down-regulated. The up-regulation of HSP20.4 suggests that there may be abnormal polypeptides produced in the lethal embryos. The gene ontology(GO)annotation indicated those down-regulated proteins are involved in important biological processes including embryo development, nucleoside metabolism, t RNA splicing, translation and protein folding. The biological pathway analysis showed that those down-regulated proteins are mainly involved in spindle assemblage and morphogenesis. Based on our results, we suggest that the l_2 may be the mutant expressing abnormal polypeptides. Its expression has a negative effect on mitosis and morphogenesis processes. The death of the embryos may be caused by the accumulation of abnormal polypeptides and the handicap of cell proliferation and morphogenesis.
The sex-linked balanced lethal(SLBL)strains of silkworm serve as an effective system for sex-control in silkworm. To gain comprehensive insight into the effect of one sex-linked balanced lethal gene l_2, comparative proteomic analysis was carried out between the survival embryos(W~(+l1)Z~(l1+l_2)) and lethal embryos(W~(+l1)Z~(+l1l_2))before the lethal stage. The lethal stage of l_2 was confirmed by observing the typical dead embryo morphology. The two genotype embryos before lethal stage were distinguished using polymorphic simple sequence repeats(SSR) markers closely linked to l_2 on the sex chromosome. Finally, 11 differentially expressed protein spots were successfully identified by MALDI-TOF/TOF mass spectrometry(MS). Among them, only 1 protein identified as heat shock protein 20.4(HSP20.4)was up-regulated in the lethal embryos, while the other 10 were down-regulated. The up-regulation of HSP20.4 suggests that there may be abnormal polypeptides produced in the lethal embryos. The gene ontology(GO)annotation indicated those down-regulated proteins are involved in important biological processes including embryo development, nucleoside metabolism, t RNA splicing, translation and protein folding. The biological pathway analysis showed that those down-regulated proteins are mainly involved in spindle assemblage and morphogenesis. Based on our results, we suggest that the l_2 may be the mutant expressing abnormal polypeptides. Its expression has a negative effect on mitosis and morphogenesis processes. The death of the embryos may be caused by the accumulation of abnormal polypeptides and the handicap of cell proliferation and morphogenesis.
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