A Comparative Proteomic Analysis of Parthenogenetic Lines and Amphigenetic Lines of Silkworm
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- 英文论文题名：A Comparative Proteomic Analysis of Parthenogenetic Lines and Amphigenetic Lines of Silkworm
- 论文作者：Peigang Liu ; Yongqiang Wang ; Xin Du ; Fangxiong Shi ; Zhiqi Meng
- 英文论文作者：Peigang Liu ; Yongqiang Wang ; Xin Du ; Fangxiong Shi ; Zhiqi Meng ; College of Animal Science and Technology ; Nanjing Agricultural University ; Sericulture Research Institute ; Zhejiang Academy of Agricultural Sciences
- 年：2016
- 作者机构：College of Animal Science and Technology, Nanjing Agricultural University;Sericulture Research Institute, Zhejiang Academy of Agricultural Sciences;
- 英文论文关键词：comparative proteomic analysis ; new-hatched silkworm ; parthenogenetic lines ; amphigenetic lines
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：447k
- 原文格式：D
- 会议级别：全国
摘要
Parthenogenetic strains of silkworm serve as an effective system for sex-control in silkworms. To determine the molecular mechanism of silkworm parthenogenesis, protein profiles from newly hatched silkworm of a parthenogenetic lines with high pigmentation rate and hatching rate were compared with amphigenetic lines using proteomics approach, including by two-dimensional electrophoresis(2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF/TOF MS), and bioinformatics analysis. Several proteins were expressed differentially between the parthenogenetic and amphigenetic lines, and seven of nine interesting proteins were identified successfully using MALDI-TOF/TOF MS analysis. The identified proteins were muscular protein-20, odorant binding protein-LOC100301497, glutathione S-transferase delta, translationally controlled tumor protein homolog, cuticular protein RR-1 motif 19, beta-actin, actins, and muscle-type A1 actins. These proteins may be associated with the regulation of growth, development, and reproductive processes of silkworm parthenogenetic lines.
Parthenogenetic strains of silkworm serve as an effective system for sex-control in silkworms. To determine the molecular mechanism of silkworm parthenogenesis, protein profiles from newly hatched silkworm of a parthenogenetic lines with high pigmentation rate and hatching rate were compared with amphigenetic lines using proteomics approach, including by two-dimensional electrophoresis(2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF/TOF MS), and bioinformatics analysis. Several proteins were expressed differentially between the parthenogenetic and amphigenetic lines, and seven of nine interesting proteins were identified successfully using MALDI-TOF/TOF MS analysis. The identified proteins were muscular protein-20, odorant binding protein-LOC100301497, glutathione S-transferase delta, translationally controlled tumor protein homolog, cuticular protein RR-1 motif 19, beta-actin, actins, and muscle-type A1 actins. These proteins may be associated with the regulation of growth, development, and reproductive processes of silkworm parthenogenetic lines.
Parthenogenetic strains of silkworm serve as an effective system for sex-control in silkworms. To determine the molecular mechanism of silkworm parthenogenesis, protein profiles from newly hatched silkworm of a parthenogenetic lines with high pigmentation rate and hatching rate were compared with amphigenetic lines using proteomics approach, including by two-dimensional electrophoresis(2-DE), matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF/TOF MS), and bioinformatics analysis. Several proteins were expressed differentially between the parthenogenetic and amphigenetic lines, and seven of nine interesting proteins were identified successfully using MALDI-TOF/TOF MS analysis. The identified proteins were muscular protein-20, odorant binding protein-LOC100301497, glutathione S-transferase delta, translationally controlled tumor protein homolog, cuticular protein RR-1 motif 19, beta-actin, actins, and muscle-type A1 actins. These proteins may be associated with the regulation of growth, development, and reproductive processes of silkworm parthenogenetic lines.
引文