Transcriptome Analysis of Thermal Parthenogenesis of the Domesticated Silkworm
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- 英文论文题名：Transcriptome Analysis of Thermal Parthenogenesis of the Domesticated Silkworm
- 论文作者：Peigang Liu ; Yongqiang Wang ; Xin Du ; Lusong Yao ; Fengbo Li ; Zhiqi Meng
- 英文论文作者：Peigang Liu ; Yongqiang Wang ; Xin Du ; Lusong Yao ; Fengbo Li ; Zhiqi Meng ; Sericultural Research Institute ; Zhejiang Academy of Agricultural Sciences ; State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control ; Sericultural Research Institute ; Zhejiang Academy of Agricultural Sciences
- 年：2016
- 作者机构：Sericultural Research Institute, Zhejiang Academy of Agricultural Sciences;State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control,Sericultural Research Institute,Zhejiang Academy of Agricultural Sciences;
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：447k
- 原文格式：D
- 会议级别：全国
摘要
Thermal induction of parthenogenesis(also known as thermal parthenogenesis) in silkworms is an important technique that has been used in artificial insemination, expansion of hybridization, transgenesis and sericultural production;however, the exact mechanisms of this induction remain unclear. This study aimed to investigate the gene expression profile in silkworms undergoing thermal parthenogenesis using RNA-seq analysis. The transcriptome profiles indicated that in non-induced and induced eggs, the numbers of differentially expressed genes(DEGs) for the parthenogenetic line(PL) and amphigenetic line(AL) were 538 and 545, respectively, as determined by fold-change≧2. Gene ontology(GO) analysis showed that DEGs between two lines were mainly involved in reproduction, formation of chorion,female gamete generation and cell development pathways. Upregulation of many chorion genes in AL suggests that the maturation rate of AL eggs was slower than PL eggs. Some DEGs related to reactive oxygen species removal, DNA repair and heat shock response were differentially expressed between the two lines, such as MPV-17, REV1 and HSP68. These results supported the view that a large fraction of genes are differentially expressed between PL and AL, which offers a new approach to identifying the molecular mechanism of silkworm thermal parthenogenesis.
Thermal induction of parthenogenesis(also known as thermal parthenogenesis) in silkworms is an important technique that has been used in artificial insemination, expansion of hybridization, transgenesis and sericultural production;however, the exact mechanisms of this induction remain unclear. This study aimed to investigate the gene expression profile in silkworms undergoing thermal parthenogenesis using RNA-seq analysis. The transcriptome profiles indicated that in non-induced and induced eggs, the numbers of differentially expressed genes(DEGs) for the parthenogenetic line(PL) and amphigenetic line(AL) were 538 and 545, respectively, as determined by fold-change≧2. Gene ontology(GO) analysis showed that DEGs between two lines were mainly involved in reproduction, formation of chorion,female gamete generation and cell development pathways. Upregulation of many chorion genes in AL suggests that the maturation rate of AL eggs was slower than PL eggs. Some DEGs related to reactive oxygen species removal, DNA repair and heat shock response were differentially expressed between the two lines, such as MPV-17, REV1 and HSP68. These results supported the view that a large fraction of genes are differentially expressed between PL and AL, which offers a new approach to identifying the molecular mechanism of silkworm thermal parthenogenesis.
Thermal induction of parthenogenesis(also known as thermal parthenogenesis) in silkworms is an important technique that has been used in artificial insemination, expansion of hybridization, transgenesis and sericultural production;however, the exact mechanisms of this induction remain unclear. This study aimed to investigate the gene expression profile in silkworms undergoing thermal parthenogenesis using RNA-seq analysis. The transcriptome profiles indicated that in non-induced and induced eggs, the numbers of differentially expressed genes(DEGs) for the parthenogenetic line(PL) and amphigenetic line(AL) were 538 and 545, respectively, as determined by fold-change≧2. Gene ontology(GO) analysis showed that DEGs between two lines were mainly involved in reproduction, formation of chorion,female gamete generation and cell development pathways. Upregulation of many chorion genes in AL suggests that the maturation rate of AL eggs was slower than PL eggs. Some DEGs related to reactive oxygen species removal, DNA repair and heat shock response were differentially expressed between the two lines, such as MPV-17, REV1 and HSP68. These results supported the view that a large fraction of genes are differentially expressed between PL and AL, which offers a new approach to identifying the molecular mechanism of silkworm thermal parthenogenesis.
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